{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T18:05:29Z","timestamp":1704823529268},"reference-count":35,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2016,1,15]],"date-time":"2016-01-15T00:00:00Z","timestamp":1452816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Dept. of Biotechnology, Govt. of India,","award":["BT\/MB\/THSTI\/HMC-SFC\/2011","BT\/IN\/DENMARK\/12\/M\/2013"],"award-info":[{"award-number":["BT\/MB\/THSTI\/HMC-SFC\/2011","BT\/IN\/DENMARK\/12\/M\/2013"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2016,1,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The genesis of toxigenic\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Vibrio cholerae<\/jats:named-content>\n            involves acquisition of CTX\u03d5, a single-stranded DNA (ssDNA) filamentous phage that encodes cholera toxin (CT). The phage exploits host-encoded tyrosine recombinases (XerC and XerD) for chromosomal integration and lysogenic conversion. The replicative genome of CTX\u03d5 produces ssDNA by rolling-circle replication, which may be used either for virion production or for integration into host chromosome. Fine-tuning of different ssDNA binding protein (Ssb) levels in the host cell is crucial for cellular functioning and important for CTX\u03d5 integration. In this study, we mutated the master regulator gene of SOS induction,\n            <jats:italic>lexA<\/jats:italic>\n            , of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">V. cholerae<\/jats:named-content>\n            because of its known role in controlling levels of Ssb proteins in other bacteria. CTX\u03d5 integration decreased in cells with a \u0394\n            <jats:italic>lexA<\/jats:italic>\n            mutation and increased in cells with an SOS-noninducing mutation,\n            <jats:italic>lexA<\/jats:italic>\n            (Ind\n            <jats:sup>\u2212<\/jats:sup>\n            ). We also observed that overexpression of host-encoded Ssb (VC0397) decreased integration of CTX\u03d5. We propose that LexA helps CTX\u03d5 integration, possibly by fine-tuning levels of host- and phage-encoded Ssbs.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            Cholera toxin is the principal virulence factor responsible for the acute diarrheal disease cholera. CT is encoded in the genome of a lysogenic filamentous phage, CTX\u03d5.\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Vibrio cholerae<\/jats:named-content>\n            has a bipartite genome and harbors single or multiple copies of CTX\u03d5 prophage in one or both chromosomes. Two host-encoded tyrosine recombinases (XerC and XerD) recognize the folded ssDNA genome of CTX\u03d5 and catalyze its integration at the dimer resolution site of either one or both chromosomes. Fine-tuning of ssDNA binding proteins in host cells is crucial for CTX\u03d5 integration. We engineered the\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">V. cholerae<\/jats:named-content>\n            genome and created several reporter strains carrying \u0394\n            <jats:italic>lexA<\/jats:italic>\n            or\n            <jats:italic>lexA<\/jats:italic>\n            (Ind\n            <jats:sup>\u2212<\/jats:sup>\n            ) alleles. Using the reporter strains, the importance of LexA control of Ssb expression in the integration efficiency of CTX\u03d5 was demonstrated.\n          <\/jats:p>","DOI":"10.1128\/jb.00674-15","type":"journal-article","created":{"date-parts":[[2015,10,27]],"date-time":"2015-10-27T02:14:23Z","timestamp":1445912063000},"page":"268-275","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Effect of LexA on Chromosomal Integration of CTX\u03d5 in Vibrio cholerae"],"prefix":"10.1128","volume":"198","author":[{"given":"Archana","family":"Pant","sequence":"first","affiliation":[{"name":"Molecular Genetics Laboratory, Centre for Human Microbial Ecology, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, Faridabad, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Anbumani","sequence":"additional","affiliation":[{"name":"Molecular Genetics Laboratory, Centre for Human Microbial Ecology, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, Faridabad, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satyabrata","family":"Bag","sequence":"additional","affiliation":[{"name":"Molecular Genetics Laboratory, Centre for Human Microbial Ecology, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, Faridabad, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ojasvi","family":"Mehta","sequence":"additional","affiliation":[{"name":"Molecular Genetics Laboratory, Centre for Human Microbial Ecology, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, Faridabad, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pawan","family":"Kumar","sequence":"additional","affiliation":[{"name":"Molecular Genetics Laboratory, Centre for Human Microbial Ecology, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, Faridabad, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shruti","family":"Saxena","sequence":"additional","affiliation":[{"name":"Molecular Genetics Laboratory, Centre for Human Microbial Ecology, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, Faridabad, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. Balakrish","family":"Nair","sequence":"additional","affiliation":[{"name":"Molecular Genetics Laboratory, Centre for Human Microbial Ecology, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, Faridabad, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhabatosh","family":"Das","sequence":"additional","affiliation":[{"name":"Molecular Genetics Laboratory, Centre for Human Microbial Ecology, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, Faridabad, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5270.1910"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2005.07.002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0910212107"},{"key":"e_1_3_2_5_2","first-page":"1","article-title":"Mechanistic insights into filamentous phage integration in Vibrio cholerae","volume":"650","author":"Das B","year":"2014","unstructured":"Das B. 2014. Mechanistic insights into filamentous phage integration in Vibrio cholerae. Front Microbiol 650:1\u20139.","journal-title":"Front Microbiol"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.3911758.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0907787106"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1111\/1574-6976.12077","article-title":"SOS, the formidable strategy of bacteria against aggressions","volume":"38","author":"Baharoglu Z","year":"2014","unstructured":"Baharoglu Z, Mazel D. 2014. SOS, the formidable strategy of bacteria against aggressions. FEMS Microbiol Rev 38:20.","journal-title":"FEMS Microbiol Rev"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00026-10"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01206-08"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1235"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1114021"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06971"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.151242898"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi201863z"},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1093\/genetics\/158.1.41","article-title":"Comparative gene expression profiles following UV exposure in wild-type and SOS-deficient Escherichia coli","volume":"158","author":"Courcelle J","year":"2001","unstructured":"Courcelle J, Khodursky A, Peter B, Brown PO, Hanawalt PC. 2001. Comparative gene expression profiles following UV exposure in wild-type and SOS-deficient Escherichia coli. Genetics 158:41\u201364.","journal-title":"Genetics"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/35020000"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-008-8378-6"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09200"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr265"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2007.00082.x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8456314"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2004.11.046"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01549-10"},{"key":"e_1_3_2_25_2","first-page":"195","article-title":"Molecular mechanism of acquisition of the cholera toxin genes","volume":"133","author":"Das B","year":"2011","unstructured":"Das B, Bischerour J, Barre FX. 2011. Molecular mechanism of acquisition of the cholera toxin genes. Indian J Med Res 133:195\u2013200.","journal-title":"Indian J Med Res"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1017061108"},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1073\/pnas.1404047111","article-title":"XerD-mediated FtsK-independent integration of TLCvarphi into the Vibrio cholerae genome","volume":"111","author":"Midonet C","year":"2014","unstructured":"Midonet C, Das B, Paly E, Barre FX. 2014. XerD-mediated FtsK-independent integration of TLCvarphi into the Vibrio cholerae genome. Proc Natl Acad Sci U S A 111:6.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00682-09"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1462"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2012.10.003"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.71.083101.133940"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01966-14"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2004.09.007"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.14.4121-4130.1995"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00793-8"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2004.02.003"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00674-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00674-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:41:49Z","timestamp":1627580509000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.00674-15"}},"subtitle":[],"editor":[{"given":"V. J.","family":"DiRita","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2016,1,15]]},"references-count":35,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,1,15]]}},"alternative-id":["10.1128\/JB.00674-15"],"URL":"https:\/\/doi.org\/10.1128\/jb.00674-15","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1,15]]},"assertion":[{"value":"2015-08-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-10-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-12-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}