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The enterobacterial Feo system is composed of 3 proteins: FeoB is the indispensable component and is a large membrane protein likely to function as a permease; FeoA is a small Src homology 3 (SH3) domain protein that interacts with FeoB; FeoC is a winged-helix protein containing 4 conserved Cys residues in a sequence suitable for harboring a putative iron-sulfur (Fe-S) cluster. The presence of an iron-sulfur cluster on FeoC has never been shown experimentally. We report that under anaerobic conditions, the recombinant<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Klebsiella pneumoniae<\/jats:named-content>FeoC (<jats:italic>Kp<\/jats:italic>FeoC) exhibited hyperfine-shifted nuclear magnetic resonance (NMR) and a UV-visible (UV-Vis) absorbance spectrum characteristic of a paramagnetic center. The electron paramagnetic resonance (EPR) and extended X-ray absorption fine structure (EXAFS) results were consistent only with the [4Fe-4S] clusters. Substituting the cysteinyl sulfur with oxygen resulted in significantly reduced cluster stability, establishing the roles of these cysteines as the ligands for the Fe-S cluster. When exposed to oxygen, the [4Fe-4S] cluster degraded to [3Fe-4S] and eventually disappeared. We propose that<jats:italic>Kp<\/jats:italic>FeoC may regulate the function of the Feo transporter through the oxygen- or iron-sensitive coordination of the Fe-S cluster.<\/jats:p>","DOI":"10.1128\/jb.00687-13","type":"journal-article","created":{"date-parts":[[2013,8,17]],"date-time":"2013-08-17T05:09:08Z","timestamp":1376716148000},"page":"4726-4734","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["FeoC from Klebsiella pneumoniae Contains a [4Fe-4S] Cluster"],"prefix":"10.1128","volume":"195","author":[{"given":"Kuang-Lung","family":"Hsueh","sequence":"first","affiliation":[{"name":"Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang-Kun","family":"Yu","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yung-Han","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Physics, National Dong Hwa University, Hualien, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Hsin","family":"Cheng","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin-Cheng","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Life Science Group, Scientific Research Division, National Synchrotron Radiation Research Center, Hsinchu, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shyue-chu","family":"Ke","sequence":"additional","affiliation":[{"name":"Department of Physics, National Dong Hwa University, Hualien, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuo-Wei","family":"Hung","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Jung","family":"Chen","sequence":"additional","affiliation":[{"name":"Life Science Group, Scientific Research Division, National Synchrotron Radiation Research Center, Hsinchu, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tai-huang","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan"},{"name":"Department of Physics, National Taiwan Normal University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1046"},{"key":"e_1_3_3_3_2","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1006\/bcmd.2002.0564","article-title":"Pathways of iron absorption","volume":"29","author":"Conrad ME","year":"2002","unstructured":"ConradMEUmbreitJN. 2002. 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