{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T01:54:03Z","timestamp":1778032443309,"version":"3.51.4"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"20","license":[{"start":{"date-parts":[[2009,10,15]],"date-time":"2009-10-15T00:00:00Z","timestamp":1255564800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2009,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            colonizes and can persist in the lungs of cystic fibrosis (CF) patients for decades. Adaptation of\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            to the CF lung environment causes various genotypic and phenotypic alterations in the bacterium that facilitate persistence. We showed previously that isocitrate lyase (ICL) activity is constitutively upregulated in the\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            CF isolate FRD1. We show here that high ICL activity in FRD1 contributes to increased hydrogen cyanide (HCN) production by this isolate. Disruption of\n            <jats:italic>aceA<\/jats:italic>\n            , which encodes ICL, results in reduced cyanide production by FRD1 but does not affect cyanide production in the wound isolate PAO1. Cyanide production is restored to the FRD1\n            <jats:italic>aceA<\/jats:italic>\n            mutant by addition of glyoxylate, a product of ICL activity, or glycine to the growth medium. Conversion of glyoxylate to glycine may provide a mechanism for increased cyanide production by\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            growing on compounds that activate the glyoxylate pathway. Consistent with this hypothesis, disruption of PA5304, encoding a putative\n            <jats:sc>d<\/jats:sc>\n            -amino acid dehydrogenase (DadA), led to decreased cyanide production by FRD1. Cyanide production was restored to the FRD1\n            <jats:italic>dadA<\/jats:italic>\n            mutant by the addition of glycine, but not glyoxylate, to the growth medium, suggesting that loss of the ability to convert glyoxylate to glycine was associated with the\n            <jats:italic>dadA<\/jats:italic>\n            mutation. This was supported by increased glycine production from toluene-treated FRD1 cells with the addition of glyoxylate compared to FRD1\n            <jats:italic>dadA<\/jats:italic>\n            cells. This study indicates a larger role for ICL in the physiology and virulence of chronic isolates of\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            than previously recognized.\n          <\/jats:p>","DOI":"10.1128\/jb.00692-09","type":"journal-article","created":{"date-parts":[[2009,8,22]],"date-time":"2009-08-22T01:47:44Z","timestamp":1250905664000},"page":"6335-6339","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Isocitrate Lyase Supplies Precursors for Hydrogen Cyanide Production in a Cystic Fibrosis Isolate of\n            <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1128","volume":"191","author":[{"given":"Jessica M.","family":"Hagins","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Auburn University, Auburn, Alabama 36849"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Locy","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Auburn University, Auburn, Alabama 36849"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Silo-Suh","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Auburn University, Auburn, Alabama 36849"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1007\/s002039900127","volume":"173","year":"2000","unstructured":"Blumer, C., and D. 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