{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:46:28Z","timestamp":1791942388276,"version":"4.3.4"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"19","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Bacterial differentiation is often associated with the asymmetric localization of regulatory proteins, such as histidine kinases. PdhS is an essential and polarly localized histidine kinase in the pathogenic alphaproteobacterium\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella abortus<\/jats:named-content>\n            . After cell division, PdhS is asymmetrically segregated between the two sibling cells, highlighting a differentiation event. However, the function(s) of PdhS in the\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. abortus<\/jats:named-content>\n            cell cycle remains unknown. We used an original approach, the pentapeptide scanning mutagenesis method, to generate a thermosensitive allele of\n            <jats:italic>pdhS<\/jats:italic>\n            . We report that a\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. abortus<\/jats:named-content>\n            strain carrying this\n            <jats:italic>pdhS<\/jats:italic>\n            allele displays growth arrest and an altered DivK-yellow fluorescent protein (YFP) polar localization at the restrictive temperature. Moreover, the production of a nonphosphorylatable PdhS protein or truncated PdhS proteins leads to dominant-negative effects by generating morphological defects consistent with the inhibition of cell division. In addition, we have used a domain mapping approach combined with yeast two-hybrid and fluorescence microscopy methods to better characterize the unusual PdhS sensory domain. We have identified a fragment of the PdhS sensory domain required for protein-protein interaction (amino acids [aa] 210 to 434), a fragment sufficient for polar localization (aa 1 to 434), and a fragment (aa 527 to 661) whose production in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. abortus<\/jats:named-content>\n            correlates with the generation of cell shape alterations. The data support a model in which PdhS acts as an essential regulator of cell cycle progression in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. abortus<\/jats:named-content>\n            and contribute to a better understanding of the differentiation program inherited by the two sibling cells.\n          <\/jats:p>","DOI":"10.1128\/jb.00699-12","type":"journal-article","created":{"date-parts":[[2012,7,29]],"date-time":"2012-07-29T00:53:02Z","timestamp":1343523182000},"page":"5305-5314","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["The Histidine Kinase PdhS Controls Cell Cycle Progression of the Pathogenic Alphaproteobacterium Brucella abortus"],"prefix":"10.1128","volume":"194","author":[{"given":"Charles","family":"Van der Henst","sequence":"first","affiliation":[{"name":"Microorganisms Biology Research Unit (URBM), University of Namur (FUNDP), Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Beaufay","sequence":"additional","affiliation":[{"name":"Microorganisms Biology Research Unit (URBM), University of Namur (FUNDP), Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johann","family":"Mignolet","sequence":"additional","affiliation":[{"name":"Microorganisms Biology Research Unit (URBM), University of Namur (FUNDP), Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Didembourg","sequence":"additional","affiliation":[{"name":"Microorganisms Biology Research Unit (URBM), University of Namur (FUNDP), Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Colinet","sequence":"additional","affiliation":[{"name":"Microorganisms Biology Research Unit (URBM), University of Namur (FUNDP), Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Hallet","sequence":"additional","affiliation":[{"name":"Institut des Sciences de la Vie, Division of Biochemistry and Molecular Genetics of Bacteria, Universit\u00e9 Catholique de Louvain, Louvain-la-Neuve, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Jacques","family":"Letesson","sequence":"additional","affiliation":[{"name":"Microorganisms Biology Research Unit (URBM), University of Namur (FUNDP), Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"De Bolle","sequence":"additional","affiliation":[{"name":"Microorganisms Biology Research Unit (URBM), University of Namur (FUNDP), Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1046\/j.1365-2958.2002.02777.x","article-title":"Plasticity of a transcriptional regulation network among alpha-proteobacteria is supported by the identification of CtrA targets in Brucella abortus","volume":"43","author":"Bellefontaine AF","year":"2002","unstructured":"BellefontaineAF. 2002. 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