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These genes encode a polar sheathed flagellum that is transiently expressed during vegetative growth and required for persistent infection in a mouse model. By following the expression of three flagellar genes (<jats:italic>fliF<\/jats:italic>,<jats:italic>flgE<\/jats:italic>, and<jats:italic>fliC<\/jats:italic>, corresponding to the MS ring, hook, and filament monomer, respectively), we identified a new regulator gene,<jats:italic>ftcR<\/jats:italic>(<jats:italic>f<\/jats:italic>lagellar<jats:italic>t<\/jats:italic>wo-<jats:italic>c<\/jats:italic>omponent<jats:italic>r<\/jats:italic>egulator). Inactivation of<jats:italic>ftcR<\/jats:italic>led to a decrease in flagellar gene expression and to impaired<jats:italic>Brucella<\/jats:italic>virulence. FtcR has a two-component response regulator domain as well a DNA binding domain and is encoded in the first flagellar locus of<jats:italic>B. melitensis<\/jats:italic>. Both the<jats:italic>ftcR<\/jats:italic>sequence and its genomic context are conserved in other related \u03b1-proteobacteria. During vegetative growth in rich medium,<jats:italic>ftcR<\/jats:italic>expression showed a peak during the early exponential phase that paralleled<jats:italic>fliF<\/jats:italic>gene expression. VjbR, a quorum-sensing regulator of the LuxR family, was previously found to control<jats:italic>fliF<\/jats:italic>and<jats:italic>flgE<\/jats:italic>gene expression. Here, we provide some new elements suggesting that the effect of VjbR on these flagellar genes is mediated by FtcR. We found that<jats:italic>ftcR<\/jats:italic>expression is partially under the control of VjbR and that the expression in<jats:italic>trans<\/jats:italic>of<jats:italic>ftcR<\/jats:italic>in a<jats:italic>vjbR<\/jats:italic>mutant restored the production of the hook protein (FlgE). Finally, FtcR binds directly to the upstream region of the<jats:italic>fliF<\/jats:italic>gene. Therefore, our data support the role of FtcR as a flagellar master regulator in<jats:italic>B. melitensis<\/jats:italic>and perhaps in other related \u03b1-proteobacteria.<\/jats:p>","DOI":"10.1128\/jb.00712-06","type":"journal-article","created":{"date-parts":[[2006,12,16]],"date-time":"2006-12-16T00:30:00Z","timestamp":1166229000000},"page":"131-141","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":43,"title":["FtcR Is a New Master Regulator of the Flagellar System of<i>Brucella melitensis<\/i>16M with Homologs in<i>Rhizobiaceae<\/i>"],"prefix":"10.1128","volume":"189","author":[{"given":"S.","family":"Le\u0301onard","sequence":"first","affiliation":[{"name":"Unite\u0301 de Recherche en Biologie Mole\u0301culaire, Laboratoire d'Immunologie-Microbiologie, Faculte\u0301s Universitaires Notre-Dame de la Paix, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Ferooz","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Recherche en Biologie Mole\u0301culaire, Laboratoire d'Immunologie-Microbiologie, Faculte\u0301s Universitaires Notre-Dame de la Paix, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Haine","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Recherche en Biologie Mole\u0301culaire, Laboratoire d'Immunologie-Microbiologie, Faculte\u0301s Universitaires Notre-Dame de la Paix, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Danese","sequence":"additional","affiliation":[{"name":"Centre d'Etude et de Recherches Ve\u0301te\u0301rinaires et Agronomiques, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Fretin","sequence":"additional","affiliation":[{"name":"Centre d'Etude et de Recherches Ve\u0301te\u0301rinaires et Agronomiques, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Tibor","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Recherche en Biologie Mole\u0301culaire, Laboratoire d'Immunologie-Microbiologie, Faculte\u0301s Universitaires Notre-Dame de la Paix, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"de Walque","sequence":"additional","affiliation":[{"name":"Service de Chimie Biologique, Institut de Biologie et de Me\u0301decine Mole\u0301culaires, Universite\u0301 Libre de Bruxelles, Gosselies, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"De Bolle","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Recherche en Biologie Mole\u0301culaire, Laboratoire d'Immunologie-Microbiologie, Faculte\u0301s Universitaires Notre-Dame de la Paix, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.-J.","family":"Letesson","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Recherche en Biologie Mole\u0301culaire, Laboratoire d'Immunologie-Microbiologie, Faculte\u0301s Universitaires Notre-Dame de la Paix, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/S1369-5274(02)00302-8","volume":"5","year":"2002","unstructured":"Aldridge, P., and K. 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