{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T12:24:25Z","timestamp":1788351865665,"version":"build-2803163510"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"24","license":[{"start":{"date-parts":[[2013,12,15]],"date-time":"2013-12-15T00:00:00Z","timestamp":1387065600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2013,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Burkholderia pseudomallei<\/jats:named-content>\n            , the causative agent of melioidosis, contains a large pathogen genome (7.2 Mb) with \u223c2,000 genes of putative or unknown function. Interactions with potential hosts and environmental factors may induce rapid adaptations in these\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. pseudomallei<\/jats:named-content>\n            genes, which can be discerned through evolutionary analysis of multiple\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. pseudomallei<\/jats:named-content>\n            genomes. Here we show that several previously uncharacterized\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. pseudomallei<\/jats:named-content>\n            genes bearing genetic signatures of rapid adaptation (positive selection) can induce diverse cellular phenotypes when expressed in mammalian cells. Notably, several of these phenotypes are plausibly related to virulence, including multinuclear giant cell formation, apoptosis, and autophagy induction. Specifically, we show that\n            <jats:italic>BPSS0180<\/jats:italic>\n            , a type VI cluster-associated gene, is capable of inducing autophagy in both phagocytic and nonphagocytic mammalian cells. Following infection of macrophages, a\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. pseudomallei<\/jats:named-content>\n            mutant disrupted in\n            <jats:italic>BPSS0180<\/jats:italic>\n            exhibited significantly decreased colocalization with LC3 and impaired intracellular survival; these phenotypes were rescued by introduction of an intact\n            <jats:italic>BPSS0180<\/jats:italic>\n            gene. The results suggest that\n            <jats:italic>BPSS0180<\/jats:italic>\n            may be a novel inducer of host cell autophagy that contributes to\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. pseudomallei<\/jats:named-content>\n            intracellular growth. More generally, our study highlights the utility of applying evolutionary principles to microbial genomes to identify novel virulence genes.\n          <\/jats:p>","DOI":"10.1128\/jb.00718-13","type":"journal-article","created":{"date-parts":[[2013,10,5]],"date-time":"2013-10-05T04:26:15Z","timestamp":1380947175000},"page":"5487-5498","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Evolutionary Analysis of Burkholderia pseudomallei Identifies Putative Novel Virulence Genes, Including a Microbial Regulator of Host Cell Autophagy"],"prefix":"10.1128","volume":"195","author":[{"given":"Arvind Pratap","family":"Singh","sequence":"first","affiliation":[{"name":"Genome Institute of Singapore, Singapore, Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-chin","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Monash University, Clayton Campus, Melbourne, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tannistha","family":"Nandi","sequence":"additional","affiliation":[{"name":"Genome Institute of Singapore, Singapore, Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui Hoon","family":"Chua","sequence":"additional","affiliation":[{"name":"Genome Institute of Singapore, Singapore, Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen Fong","family":"Ooi","sequence":"additional","affiliation":[{"name":"Genome Institute of Singapore, Singapore, Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Ong","sequence":"additional","affiliation":[{"name":"Defense Medical and Environmental Research Institute, DSO National Laboratories, Singapore, Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John D.","family":"Boyce","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Monash University, Clayton Campus, Melbourne, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Adler","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Monash University, Clayton Campus, Melbourne, Victoria, Australia"},{"name":"ARC Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, Clayton Campus, Melbourne, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodney J.","family":"Devenish","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Monash University, Clayton Campus, Melbourne, Victoria, Australia"},{"name":"ARC Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, Clayton Campus, Melbourne, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Tan","sequence":"additional","affiliation":[{"name":"Genome Institute of Singapore, Singapore, Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1385"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0403302101"},{"key":"e_1_3_3_4_2","doi-asserted-by":"crossref","first-page":"170","DOI":"10.3389\/fmicb.2011.00170","article-title":"Strategies for intracellular survival of Burkholderia pseudomallei","volume":"2","author":"Allwood EM","year":"2011","unstructured":"AllwoodEMDevenishRJPrescottMAdlerBBoyceJD. 2011. 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