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Nasal colonization triggers IgG responses against staphylococcal surface antigens; however, these antibodies cannot prevent subsequent colonization or disease. Here, we describe\n                    <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n                    WU1, a multilocus sequence type 88 (ST88) isolate that persistently colonizes the nasopharynx in mice. We report that staphylococcal protein A (SpA) is required for persistence of\n                    <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n                    WU1 in the nasopharynx. Compared to animals colonized by wild-type\n                    <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n                    , mice colonized with the \u0394\n                    <jats:italic>spa<\/jats:italic>\n                    variant mount increased IgG responses against staphylococcal colonization determinants. Immunization of mice with a nontoxigenic SpA variant, which cannot cross-link B cell receptors and divert antibody responses, elicits protein A-neutralizing antibodies that promote IgG responses against colonizing\n                    <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n                    and diminish pathogen persistence.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>IMPORTANCE<\/jats:bold>\n                    <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n                    persistently colonizes the nasopharynx in about one-third of the human population, thereby promoting community- and hospital-acquired infections. Antibiotics are currently used for decolonization of individuals at increased risk of infection. However, the efficacy of antibiotics is limited by recolonization and selection for drug-resistant strains. Here, we propose a model of how staphylococcal protein A (SpA), a B cell superantigen, modifies host immune responses during colonization to support continued persistence of\n                    <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n                    in the nasopharynx. We show that this mechanism can be thwarted by vaccine-induced anti-SpA antibodies that promote IgG responses against staphylococcal antigens and diminish colonization.\n                  <\/jats:p>","DOI":"10.1128\/jb.00735-17","type":"journal-article","created":{"date-parts":[[2018,2,13]],"date-time":"2018-02-13T06:45:26Z","timestamp":1518504326000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["Staphylococcal Protein A Contributes to Persistent Colonization of Mice with Staphylococcus aureus"],"prefix":"10.1128","volume":"200","author":[{"given":"Yan","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Emolo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Chicago, Chicago, Illinois, 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