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Here, we show that the nitrogen regulatory NtrC proteins from\n            <jats:italic>P. putida<\/jats:italic>\n            and\n            <jats:italic>Escherichia coli<\/jats:italic>\n            are functionally equivalent and that substitutions leading to partially active forms of enterobacterial NtrC provoke the same phenotypes in\n            <jats:italic>P. putida<\/jats:italic>\n            NtrC.\n            <jats:italic>P. putida<\/jats:italic>\n            has only a single P\n            <jats:sub>II<\/jats:sub>\n            -like protein, encoded by\n            <jats:italic>glnK<\/jats:italic>\n            , whose expression is nitrogen regulated. Two contiguous NtrC binding sites located upstream of the \u03c3\n            <jats:sup>N<\/jats:sup>\n            -dependent\n            <jats:italic>glnK<\/jats:italic>\n            promoter have been identified by footprinting analysis. In vitro experiments with purified proteins demonstrated that\n            <jats:italic>glnK<\/jats:italic>\n            transcription was directly activated by NtrC and that open complex formation at this promoter required integration host factor. Transcription of genes orthologous to enterobacterial\n            <jats:italic>codB<\/jats:italic>\n            ,\n            <jats:italic>dppA<\/jats:italic>\n            , and\n            <jats:italic>ureD<\/jats:italic>\n            genes, whose transcription is dependent on \u03c3\n            <jats:sup>70<\/jats:sup>\n            and which are activated by Nac in\n            <jats:italic>E. coli<\/jats:italic>\n            , has also been analyzed for\n            <jats:italic>P. putida<\/jats:italic>\n            . Whereas\n            <jats:italic>dppA<\/jats:italic>\n            does not appear to be regulated by nitrogen via NtrC, the\n            <jats:italic>codB<\/jats:italic>\n            and\n            <jats:italic>ureD<\/jats:italic>\n            genes have \u03c3\n            <jats:sup>N<\/jats:sup>\n            -dependent promoters and their nitrogen regulation was exerted directly by NtrC, thus avoiding the need for Nac, which is missing in this bacterial species. Based upon these results, we propose a simplified nitrogen regulatory network in\n            <jats:italic>P. putida<\/jats:italic>\n            (compared to that in enterobacteria), which involves an indirect-feedback autoregulation of\n            <jats:italic>glnK<\/jats:italic>\n            using NtrC as an intermediary.\n          <\/jats:p>","DOI":"10.1128\/jb.00744-09","type":"journal-article","created":{"date-parts":[[2009,8,1]],"date-time":"2009-08-01T01:10:02Z","timestamp":1249089002000},"page":"6123-6135","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":72,"title":["NtrC-Dependent Regulatory Network for Nitrogen Assimilation in\n            <i>Pseudomonas putida<\/i>"],"prefix":"10.1128","volume":"191","author":[{"given":"Ana B.","family":"Herva\u0301s","sequence":"first","affiliation":[{"name":"Centro Andaluz de Biologi\u0301a del Desarrollo, Universidad Pablo de Olavide\/CSIC, Carretera de Utrera, Km. 1, 41013 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ine\u0301s","family":"Canosa","sequence":"additional","affiliation":[{"name":"Centro Andaluz de Biologi\u0301a del Desarrollo, Universidad Pablo de Olavide\/CSIC, Carretera de Utrera, Km. 1, 41013 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Little","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ray","family":"Dixon","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Santero","sequence":"additional","affiliation":[{"name":"Centro Andaluz de Biologi\u0301a del Desarrollo, Universidad Pablo de Olavide\/CSIC, Carretera de Utrera, Km. 1, 41013 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.65.1.80-105.2001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.19.5358-5363.2002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.19.5364-5375.2002"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1046\/j.1365-2958.1998.00932.x","volume":"29","year":"1998","unstructured":"Atkinson, M. 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