{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T12:09:05Z","timestamp":1784117345936,"version":"3.55.0"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["F32GM109661"],"award-info":[{"award-number":["F32GM109661"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["U19AI107792"],"award-info":[{"award-number":["U19AI107792"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI107159"],"award-info":[{"award-number":["R01AI107159"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Brucella abortus<\/jats:named-content>\n            \u03c3\n            <jats:sup>E1<\/jats:sup>\n            is an EcfG family sigma factor that regulates the transcription of dozens of genes in response to diverse stress conditions and is required for maintenance of chronic infection in a mouse model. A putative ATP-binding cassette transporter operon,\n            <jats:italic>bab1_0223-bab1_0226<\/jats:italic>\n            , is among the most highly activated gene sets in the \u03c3\n            <jats:sup>E1<\/jats:sup>\n            regulon. The proteins encoded by the operon resemble quaternary ammonium-compatible solute importers but are most similar in sequence to the broadly conserved YehZYXW system, which remains largely uncharacterized. Transcription of\n            <jats:italic>yehZYXW<\/jats:italic>\n            is activated by the general stress sigma factor \u03c3\n            <jats:sup>S<\/jats:sup>\n            in\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            , which suggests a functional role for this transport system in bacterial stress response across the classes\n            <jats:named-content content-type=\"genus-species\">Alphaproteobacteria<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Gammaproteobacteria<\/jats:named-content>\n            . We present evidence that\n            <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n            YehZYXW does not function as an importer of known compatible solutes under physiological conditions and does not contribute to the virulence defect of a \u03c3\n            <jats:sup>E1<\/jats:sup>\n            -null strain. The sole\n            <jats:italic>in vitro<\/jats:italic>\n            phenotype associated with genetic disruption of this putative transport system is reduced growth in the presence of high Li\n            <jats:sup>+<\/jats:sup>\n            ion concentrations. A crystal structure of\n            <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n            YehZ revealed a class II periplasmic binding protein fold with significant structural homology to\n            <jats:named-content content-type=\"genus-species\">Archaeoglobus fulgidus<\/jats:named-content>\n            ProX, which binds glycine betaine. However, the structure of the YehZ ligand-binding pocket is incompatible with high-affinity binding to glycine betaine. This is consistent with weak measured binding of YehZ to glycine betaine and related compatible solutes. We conclude that YehZYXW is a conserved, stress-regulated transport system that is phylogenetically and functionally distinct from quaternary ammonium-compatible solute importers.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            <jats:named-content content-type=\"genus-species\">Brucella abortus<\/jats:named-content>\n            \u03c3\n            <jats:sup>E1<\/jats:sup>\n            regulates transcription in response to stressors encountered in its mammalian host and is necessary for maintenance of chronic infection in a mouse model. The functions of the majority of genes regulated by \u03c3\n            <jats:sup>E1<\/jats:sup>\n            remain undefined. We present a functional\/structural analysis of a conserved putative membrane transport system (YehZYXW) whose expression is strongly activated by \u03c3\n            <jats:sup>E1<\/jats:sup>\n            . Though annotated as a quaternary ammonium osmolyte uptake system, experimental physiological studies and measured ligand-binding properties of the periplasmic binding protein (PBP), YehZ, are inconsistent with this function. A crystal structure of\n            <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n            YehZ provides molecular insight into differences between bona fide quaternary ammonium osmolyte importers and YehZ-related proteins, which form a distinct phylogenetic and functional group of PBPs.\n          <\/jats:p>","DOI":"10.1128\/jb.00746-16","type":"journal-article","created":{"date-parts":[[2016,12,23]],"date-time":"2016-12-23T18:14:37Z","timestamp":1482516877000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Conserved ABC Transport System Regulated by the General Stress Response Pathways of Alpha- and Gammaproteobacteria"],"prefix":"10.1128","volume":"199","author":[{"given":"Julien","family":"Herrou","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois, USA"},{"name":"Howard Taylor Ricketts Laboratory, University of Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan W.","family":"Willett","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois, USA"},{"name":"Howard Taylor Ricketts Laboratory, University of Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel M.","family":"Czy\u017c","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois, USA"},{"name":"Howard Taylor Ricketts Laboratory, University of Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyorgy","family":"Babnigg","sequence":"additional","affiliation":[{"name":"Argonne National Laboratory, Argonne, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youngchang","family":"Kim","sequence":"additional","affiliation":[{"name":"Argonne National Laboratory, Argonne, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean","family":"Crosson","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois, USA"},{"name":"Howard Taylor Ricketts Laboratory, University of Chicago, Chicago, Illinois, USA"},{"name":"Department of Microbiology, University of Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07602.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.22.7549-7553.2005"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2013.00090"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.53.1.121-147.1989"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050649"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2002.tb00598.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.201411296"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-003-3336-9"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2004.06.005"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(98)92003-1"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2646"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00031-07"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(01)01193-7"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST20150047"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.08-121855"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2015.09.007"},{"key":"e_1_3_3_18_2","first-page":"221","article-title":"Sigma (s)-dependent regulation of yehZYXW, which encodes a putative osmoprotectant ABC transporter of Escherichia coli","volume":"236","author":"Checroun C","year":"2004","unstructured":"Checroun C Gutierrez C . 2004. 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