{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T20:50:46Z","timestamp":1759179046459,"version":"3.40.5"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"15","license":[{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R21 AI115303"],"award-info":[{"award-number":["R21 AI115303"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006955","name":"Office of Extramural Research, National Institutes of Health","doi-asserted-by":"publisher","award":["R21 AI115023"],"award-info":[{"award-number":["R21 AI115023"]}],"id":[{"id":"10.13039\/100006955","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006823","name":"Boston Children's Hospital","doi-asserted-by":"publisher","award":["Technology Development Fund"],"award-info":[{"award-number":["Technology Development Fund"]}],"id":[{"id":"10.13039\/100006823","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:p>\n            Diarrheal disease is the most common infection afflicting children worldwide. In resource-poor settings, these infections are correlated with cognitive delay, stunted growth, and premature death. With the development of efficacious, affordable, and easily administered vaccines, such infections could be prevented. While a major focus of research on biofilms has been their elimination, here we harness the bacterial biofilm to create a customizable platform for cost-effective, whole-cell mucosal vaccines that self-incorporate secreted protein antigens. We use this platform to develop a sublingually administered live-attenuated prototype vaccine based on\n            <jats:named-content content-type=\"genus-species\">Vibrio cholerae<\/jats:named-content>\n            . This serves not only as a proof of concept for a multivalent vaccine against common bacterial enteric pathogens but also as a paradigm for vaccines utilizing other bacterial biofilms to target mucosal infections.\n          <\/jats:p>","DOI":"10.1128\/jb.00752-17","type":"journal-article","created":{"date-parts":[[2018,2,27]],"date-time":"2018-02-27T11:06:08Z","timestamp":1519729568000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["A Self-Assembling Whole-Cell Vaccine Antigen Presentation Platform"],"prefix":"10.1128","volume":"200","author":[{"given":"Julie","family":"Liao","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel R.","family":"Smith","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f3hanna","family":"Brynjarsd\u00f3ttir","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9173-8662","authenticated-orcid":false,"given":"Paula I.","family":"Watnick","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA"},{"name":"Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)60844-2"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01624.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.7.4028"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1512424112"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00127-12"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002210"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1222981"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.3.1049-1059.2006"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00374-13"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0082458"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004167"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(00)00083-9"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.07.015"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw145"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/toxins9120379"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.10.1.167-172.1974"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.1.343-345.1996"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.05262-11"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.7.2689-2696.1995"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.1.221-226.2000"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-007-9594-0"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2015.02.014"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.09.073"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2006.001001.x"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2015.00014"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/006676-0"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00248-16"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803740"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.8.3118-3125.1997"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.06.040"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.2.6.685-688.1995"},{"key":"e_1_3_3_33_2","volume-title":"Guide for the care and use of laboratory animals","author":"National Research Council","year":"2011","unstructured":"National Research Council. 2011. Guide for the care and use of laboratory animals, 8th ed. National Academies Press, Washington, DC.","edition":"8"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00752-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00752-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:45:40Z","timestamp":1627580740000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.00752-17"}},"subtitle":[],"editor":[{"given":"Victor J.","family":"DiRita","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2018,8]]},"references-count":32,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2018,8]]}},"alternative-id":["10.1128\/JB.00752-17"],"URL":"https:\/\/doi.org\/10.1128\/jb.00752-17","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"type":"print","value":"0021-9193"},{"type":"electronic","value":"1098-5530"}],"subject":[],"published":{"date-parts":[[2018,8]]},"assertion":[{"value":"2017-12-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-02-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}