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During replication, these telomeres are processed by the essential telomere resolvase, ResT, in a unique biochemical reaction known as telomere resolution. In this study, we report the identification of the<jats:italic>B. hermsii resT<\/jats:italic>gene through cross-species hybridization. Sequence comparison of the<jats:italic>B. hermsii<\/jats:italic>protein with the<jats:italic>B. burgdorferi<\/jats:italic>orthologue revealed 67% identity, including all the regions currently known to be crucial for telomere resolution. In vitro studies, however, indicated that<jats:italic>B. hermsii<\/jats:italic>ResT was unable to process a replicated<jats:italic>B. burgdorferi<\/jats:italic>type 2 telomere substrate. In contrast, in vivo cross-species complementation in which the native<jats:italic>resT<\/jats:italic>gene of<jats:italic>B. burgdorferi<\/jats:italic>was replaced with<jats:italic>B. hermsii resT<\/jats:italic>had no discernible effect, even though<jats:italic>B. burgdorferi<\/jats:italic>strain B31 carries at least two type 2 telomere ends. The<jats:italic>B. burgdorferi<\/jats:italic>ResT protein was also able to process two telomere spacing mutants in vivo that were unresolvable in vitro. The unexpected differential telomere processing in vivo versus in vitro by the two telomere resolvases suggests the presence of one or more accessory factors in vivo that are normally involved in the reaction. Our current results are also expected to facilitate further studies into ResT structure and function, including possible interaction with other<jats:italic>Borrelia<\/jats:italic>proteins.<\/jats:p>","DOI":"10.1128\/jb.00760-06","type":"journal-article","created":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T23:23:35Z","timestamp":1161213815000},"page":"7378-7386","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Differential Telomere Processing by<i>Borrelia<\/i>Telomere Resolvases In Vitro but Not In Vivo"],"prefix":"10.1128","volume":"188","author":[{"given":"Yvonne","family":"Tourand","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology and Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta T2N 4N1, Canada"},{"name":"Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Troy","family":"Bankhead","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology and Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta T2N 4N1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra L.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology and Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta T2N 4N1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrienne D.","family":"Putteet-Driver","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Molecular Genetics and Medicine, University of California Irvine, Irvine, California 92697-4025"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan G.","family":"Barbour","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Molecular Genetics and Medicine, University of California Irvine, Irvine, California 92697-4025"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca","family":"Byram","sequence":"additional","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia A.","family":"Rosa","sequence":"additional","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Chaconas","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology and Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta T2N 4N1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/S0092-8674(02)00624-4","volume":"108","year":"2002","unstructured":"Aussel, L., F. 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