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The<jats:italic>S. agalactiae<\/jats:italic>hemolysin is regarded as an important virulence factor, and hemolysin expression is dependent on the<jats:italic>cyl<\/jats:italic>gene cluster.<jats:italic>cylA<\/jats:italic>and<jats:italic>cylB<\/jats:italic>encode the ATP binding and transmembrane domains of a typical ATP binding cassette (ABC) transporter. The deduced proteins contain the signature sequence of a multidrug resistance (MDR) transporter, and mutation of the genes results in a nonhemolytic and nonpigmented phenotype. To further elucidate the function of the putative transporter, nonpolar deletion mutants of<jats:italic>cylA<\/jats:italic>were constructed. These mutants are nonhemolytic and can be complemented by the transporter genes. Wild-type strain and nonhemolytic<jats:italic>cylA<\/jats:italic>and<jats:italic>cylK<\/jats:italic>deletion mutants were exposed to known substrates of MDR transporters. Mutation of<jats:italic>cylA<\/jats:italic>significantly impaired growth in the presence of daunorubicin, doxorubicin, and rhodamine 6G and resulted in a decreased export of doxorubicin from the cells. The mutation of<jats:italic>cylK<\/jats:italic>, a gene of unknown function located downstream from<jats:italic>cylA<\/jats:italic>, caused a loss of hemolysis but had no effect on the transport of MDR substrates. Furthermore, the hemolytic activity of the wild-type strain was inhibited by reserpine in a dose-dependent manner. We conclude that CylAB closely resembles an ABC-type MDR transporter and propose that the GBS hemolysin molecule represents a natural substrate of the transporter.<\/jats:p>","DOI":"10.1128\/jb.00768-05","type":"journal-article","created":{"date-parts":[[2006,8,3]],"date-time":"2006-08-03T00:08:39Z","timestamp":1154563719000},"page":"5984-5992","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["Transport of Multidrug Resistance Substrates by the<i>Streptococcus agalactiae<\/i>Hemolysin Transporter"],"prefix":"10.1128","volume":"188","author":[{"given":"Birgit","family":"Gottschalk","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Hygiene, University of Ulm, Robert Koch Strasse 8, D-89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerd","family":"Bro\u0308ker","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Hygiene, University of Ulm, Robert Koch Strasse 8, D-89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Kuhn","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Hygiene, University of Ulm, Robert Koch Strasse 8, D-89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Aymanns","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Hygiene, University of Ulm, Robert Koch Strasse 8, D-89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ute","family":"Gleich-Theurer","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Hygiene, University of Ulm, Robert Koch Strasse 8, D-89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Spellerberg","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Hygiene, University of Ulm, Robert Koch Strasse 8, D-89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"11086","DOI":"10.1016\/S0021-9258(18)82095-6","volume":"268","year":"1993","unstructured":"Ahmed, M., C. 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