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The capability to thrive in phagocytic hosts is dependent on the Dot\/Icm type IV secretion system (T4SS), which translocates multiple effector proteins into the host cell. In this study, we determined the draft genome sequence of<jats:italic>L. pneumophila<\/jats:italic>strain 130b (Wadsworth). We found that the 130b genome encodes a unique set of T4SSs, namely, the Dot\/Icm T4SS, a Trb-1-like T4SS, and two Lvh T4SS gene clusters. Sequence analysis substantiated that a core set of 107 Dot\/Icm T4SS effectors was conserved among the sequenced<jats:italic>L. pneumophila<\/jats:italic>strains Philadelphia-1, Lens, Paris, Corby, Alcoy, and 130b. We also identified new effector candidates and validated the translocation of 10 novel Dot\/Icm T4SS effectors that are not present in<jats:italic>L. pneumophila<\/jats:italic>strain Philadelphia-1. We examined the prevalence of the new effector genes among 87 environmental and clinical<jats:italic>L. pneumophila<\/jats:italic>isolates. Five of the new effectors were identified in 34 to 62% of the isolates, while less than 15% of the strains tested positive for the other five genes. Collectively, our data show that the core set of conserved Dot\/Icm T4SS effector proteins is supplemented by a variable repertoire of accessory effectors that may partly account for differences in the virulences and prevalences of particular<jats:italic>L. pneumophila<\/jats:italic>strains.<\/jats:p>","DOI":"10.1128\/jb.00778-10","type":"journal-article","created":{"date-parts":[[2010,9,11]],"date-time":"2010-09-11T03:09:05Z","timestamp":1284174545000},"page":"6001-6016","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":106,"title":["<i>Legionella pneumophila<\/i>Strain 130b Possesses a Unique Combination of Type IV Secretion Systems and Novel Dot\/Icm Secretion System Effector Proteins"],"prefix":"10.1128","volume":"192","author":[{"given":"Gunnar N.","family":"Schroeder","sequence":"first","affiliation":[{"name":"Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College London, London SW7 2AZ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola K.","family":"Petty","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Research, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aure\u0301lie","family":"Mousnier","sequence":"additional","affiliation":[{"name":"Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College London, London SW7 2AZ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clare R.","family":"Harding","sequence":"additional","affiliation":[{"name":"Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College London, London SW7 2AZ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam J.","family":"Vogrin","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryan","family":"Wee","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Research, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norman K.","family":"Fry","sequence":"additional","affiliation":[{"name":"Respiratory and Systemic Infection Laboratory, Health Protection Agency Centre for Infections, London, NW9 5EQ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy G.","family":"Harrison","sequence":"additional","affiliation":[{"name":"Respiratory and Systemic Infection Laboratory, Health Protection Agency Centre for Infections, London, NW9 5EQ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayley J.","family":"Newton","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas R.","family":"Thomson","sequence":"additional","affiliation":[{"name":"Pathogen Genomics, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott A.","family":"Beatson","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Research, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon","family":"Dougan","sequence":"additional","affiliation":[{"name":"Pathogen Genomics, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth L.","family":"Hartland","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gad","family":"Frankel","sequence":"additional","affiliation":[{"name":"Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College London, London SW7 2AZ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1099\/mic.0.25980-0","volume":"149","year":"2003","unstructured":"Alli, O. 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