{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:44:33Z","timestamp":1786581873655,"version":"build-2736575974"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Pasteurella multocida<\/jats:named-content>\n            is a Gram-negative multispecies pathogen and the causative agent of fowl cholera, a serious disease of poultry which can present in both acute and chronic forms. The major outer membrane component lipopolysaccharide (LPS) is both an important virulence factor and a major immunogen. Our previous studies determined the LPS structures expressed by different\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. multocida<\/jats:named-content>\n            strains and revealed that a number of strains belonging to different serovars contain the same LPS biosynthesis locus but express different LPS structures due to mutations within glycosyltransferase genes. In this study, we report the full LPS structure of the serovar 4 type strain, P1662, and reveal that it shares the same LPS outer core biosynthesis locus, L3, with the serovar 3 strains P1059 and Pm70. Using directed mutagenesis, the role of each glycosyltransferase gene in LPS outer core assembly was determined. LPS structural analysis of 23 Australian field isolates that contain the L3 locus revealed that at least six different LPS outer core structures can be produced as a result of mutations within the LPS glycosyltransferase genes. Moreover, some field isolates produce multiple but related LPS glycoforms simultaneously, and three LPS outer core structures are remarkably similar to the globo series of vertebrate glycosphingolipids. Our in-depth analysis showing the genetics and full range of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. multocida<\/jats:named-content>\n            lipopolysaccharide structures will facilitate the improvement of typing systems and the prediction of the protective efficacy of vaccines.\n          <\/jats:p>","DOI":"10.1128\/jb.00779-13","type":"journal-article","created":{"date-parts":[[2013,8,24]],"date-time":"2013-08-24T01:47:55Z","timestamp":1377308875000},"page":"4854-4864","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Pasteurella multocida Heddleston Serovar 3 and 4 Strains Share a Common Lipopolysaccharide Biosynthesis Locus but Display both Inter- and Intrastrain Lipopolysaccharide Heterogeneity"],"prefix":"10.1128","volume":"195","author":[{"given":"Marina","family":"Harper","sequence":"first","affiliation":[{"name":"Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Melbourne, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"St. Michael","sequence":"additional","affiliation":[{"name":"Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marietta","family":"John","sequence":"additional","affiliation":[{"name":"Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Melbourne, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgeny","family":"Vinogradov","sequence":"additional","affiliation":[{"name":"Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer A.","family":"Steen","sequence":"additional","affiliation":[{"name":"Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Melbourne, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lieke","family":"van Dorsten","sequence":"additional","affiliation":[{"name":"Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason A.","family":"Steen","sequence":"additional","affiliation":[{"name":"Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Melbourne, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Conny","family":"Turni","sequence":"additional","affiliation":[{"name":"Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Dutton Park, Brisbane, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick J.","family":"Blackall","sequence":"additional","affiliation":[{"name":"Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Dutton Park, Brisbane, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Adler","sequence":"additional","affiliation":[{"name":"Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Melbourne, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew D.","family":"Cox","sequence":"additional","affiliation":[{"name":"Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John D.","family":"Boyce","sequence":"additional","affiliation":[{"name":"Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Melbourne, Australia"},{"name":"Department of Microbiology, Monash University, Melbourne, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"1","article-title":"Pasteurella multocida: diseases and pathogenesis","volume":"361","author":"Wilkie IW","year":"2012","unstructured":"WilkieIWHarperMBoyceJDAdlerB. 2012. 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