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During the transition from an environmental saprophyte to a mammalian pathogen,\n                    <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n                    encounters and responds to rapidly changing environmental conditions. Environmental sensing systems that control cellular levels of cyclic di-GMP promote pathogen survival in diverse environments. Cyclic di-GMP controls biofilm production, virulence factors, and motility in many bacteria. This study is an evaluation of cyclic di-GMP-associated genes that are predicted to metabolize and interact with cyclic di-GMP as identified from the annotated genome of\n                    <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n                    1026b. Mutants containing transposon disruptions in each of these genes were characterized for biofilm formation and motility at two temperatures that reflect conditions that the bacteria encounter in the environment and during the infection of a mammalian host. Mutants with transposon insertions in a known phosphodiesterase (\n                    <jats:italic>cdpA<\/jats:italic>\n                    ) and a predicted hydrolase (Bp1026b_I2285) gene exhibited decreased motility regardless of temperature. In contrast, the phenotypes exhibited by mutants with transposon insertion mutations in a predicted diguanylate cyclase gene (Bp1026b_II2523) were strikingly influenced by temperature and were dependent on a conserved GG(D\/E)EF motif. The transposon insertion mutant exhibited enhanced biofilm formation at 37\u00b0C but impaired biofilm formation at 30\u00b0C. These studies illustrate the importance of studying behaviors regulated by cyclic di-GMP under varied environmental conditions in order to better understand cyclic di-GMP signaling in bacterial pathogens.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>IMPORTANCE<\/jats:bold>\n                    This report evaluates predicted cyclic di-GMP binding and metabolic proteins from\n                    <jats:named-content content-type=\"genus-species\">Burkholderia pseudomallei<\/jats:named-content>\n                    1026b, a tier 1 select agent and the etiologic agent of melioidosis. Transposon insertion mutants with disruptions in each of the genes encoding these predicted proteins were characterized in order to identify key components of the\n                    <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n                    cyclic di-GMP-signaling network. A predicted hydrolase and a phosphodiesterase that modulate swimming motility were identified, in addition to a diguanylate cyclase that modulates biofilm formation and motility in response to temperature. These studies warrant further evaluation of the contribution of cyclic di-GMP to melioidosis in the context of pathogen acquisition from environmental reservoirs and subsequent colonization, dissemination, and persistence within the host.\n                  <\/jats:p>","DOI":"10.1128\/jb.00780-16","type":"journal-article","created":{"date-parts":[[2016,12,12]],"date-time":"2016-12-12T21:30:34Z","timestamp":1481578234000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["Thermoregulation of Biofilm Formation in Burkholderia pseudomallei Is Disrupted by Mutation of a Putative Diguanylate Cyclase"],"prefix":"10.1128","volume":"199","author":[{"given":"Brooke A.","family":"Plumley","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin 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