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The polysaccharides of both forms are comprised of the amino sugars\n            <jats:italic>N<\/jats:italic>\n            -acetyl-\n            <jats:sc>d<\/jats:sc>\n            -glucosamine (GlcNAc),\n            <jats:italic>N<\/jats:italic>\n            -acetyl-\n            <jats:sc>d<\/jats:sc>\n            -mannosaminuronic acid (ManNAcA), and 4-acetamido-4,6-dideoxy-\n            <jats:sc>d<\/jats:sc>\n            -galactose (Fuc4NAc). These amino sugars are linked to one another to form trisaccharide repeat units with the structure \u21923-\u03b1-\n            <jats:sc>d<\/jats:sc>\n            -Fuc4NAc-(1\u21924)-\u03b2-\n            <jats:sc>d<\/jats:sc>\n            -ManNAcA-(1\u21924)-\u03b1-\n            <jats:sc>d<\/jats:sc>\n            -GlcNAc-(1\u2192. The hydroxyl group in the 6 position of the GlcNAc residues of both ECA\n            <jats:sub>CYC<\/jats:sub>\n            and ECA\n            <jats:sub>PG<\/jats:sub>\n            are nonstoichiometrically esterified with acetyl groups. Random transposon insertion mutagenesis of\n            <jats:italic>E. coli<\/jats:italic>\n            K-12 resulted in the generation of a mutant defective in the incorporation of\n            <jats:italic>O<\/jats:italic>\n            -acetyl groups into both ECA\n            <jats:sub>CYC<\/jats:sub>\n            and ECA\n            <jats:sub>PG<\/jats:sub>\n            . This defect was found to be due to an insertion of the transposon into the\n            <jats:italic>yiaH<\/jats:italic>\n            locus, a putative gene of unknown function located at 80.26 min on the\n            <jats:italic>E. coli<\/jats:italic>\n            chromosomal map. Bioinformatic analyses of the predicted\n            <jats:italic>yiaH<\/jats:italic>\n            gene product indicate that it is an integral inner membrane protein that is a member of an acyltransferase family of enzymes found in a wide variety of organisms. The results of biochemical and genetic experiments presented here strongly support the conclusion that\n            <jats:italic>yiaH<\/jats:italic>\n            encodes the\n            <jats:italic>O<\/jats:italic>\n            -acetyltransferase responsible for the incorporation of\n            <jats:italic>O<\/jats:italic>\n            -acetyl groups into both ECA\n            <jats:sub>CYC<\/jats:sub>\n            and ECA\n            <jats:sub>PG<\/jats:sub>\n            . Accordingly, we propose that this gene be designated\n            <jats:italic>wecH<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jb.00783-06","type":"journal-article","created":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T23:23:35Z","timestamp":1161213815000},"page":"7542-7550","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["O Acetylation of the Enterobacterial Common Antigen Polysaccharide Is Catalyzed by the Product of the\n            <i>yiaH<\/i>\n            Gene of\n            <i>Escherichia coli<\/i>\n            K-12"],"prefix":"10.1128","volume":"188","author":[{"given":"Junko","family":"Kajimura","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arifur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew R.","family":"Evans","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75390-8816"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin H.","family":"Gardner","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75390-8816"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul D.","family":"Rick","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/S0378-1119(97)00083-8","volume":"192","year":"1997","unstructured":"Achtman, M. 1997. 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