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We studied the role of iron and other metals in the binding and transport of Pvd by FpvA and conclude that there is no significant affinity between FpvA and metal-free Pvd. We found that the fluorescent in vivo complex of iron-free FpvA-Pvd is in fact a complex with aluminum (FpvA-Pvd-Al) formed from trace aluminum in the growth medium. When<jats:italic>Pseudomonas aeruginosa<\/jats:italic>was cultured in a medium that had been treated with a metal affinity resin, the in vivo formation of the FpvA-Pvd complex and the recycling of Pvd on FpvA were nearly abolished. The accumulation of Pvd in the periplasm of<jats:italic>Pseudomonas aeruginosa<\/jats:italic>was also reduced in the treated growth medium, while the addition of 1 \u03bcM AlCl<jats:sub>3<\/jats:sub>to the treated medium restored the effects of trace metals observed in standard growth medium. Using fluorescent resonance energy transfer and surface plasmon resonance techniques, the in vitro interactions between Pvd and detergent-solubilized FpvA were also shown to be metal dependent. We demonstrated that FpvA binds Pvd-Fe but not Pvd and that Pvd did not compete with Pvd-Fe for FpvA binding. In light of our finding that the Pvd-Al complex is transported across the outer membrane of<jats:italic>Pseudomonas aeruginosa<\/jats:italic>, a model for siderophore recognition based on a metal-induced conformation followed by redox selectivity for iron is discussed.<\/jats:p>","DOI":"10.1128\/jb.00784-08","type":"journal-article","created":{"date-parts":[[2008,7,19]],"date-time":"2008-07-19T00:54:50Z","timestamp":1216428890000},"page":"6548-6558","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["The Metal Dependence of Pyoverdine Interactions with Its Outer Membrane Receptor FpvA"],"prefix":"10.1128","volume":"190","author":[{"given":"Jason","family":"Greenwald","sequence":"first","affiliation":[{"name":"De\u0301partement Re\u0301cepteurs et Prote\u0301ines Membranaires"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabrielle","family":"Zeder-Lutz","sequence":"additional","affiliation":[{"name":"De\u0301partement Biotechnologie des Interactions Macromole\u0301culaires, Institut Gilbert-Laustriat, UMR 7175-LC1 CNRS, ESBS, Blvd. Se\u0301bastien Brant, F-67413 Illkirch, Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agne\u0300s","family":"Hagege","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie Analytique et Sciences Se\u0301paratives, IPHC-DSA (UMR7178) ECPM, 25 rue Becquerel, 67087 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herve\u0301","family":"Celia","sequence":"additional","affiliation":[{"name":"De\u0301partement Re\u0301cepteurs et Prote\u0301ines Membranaires"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc","family":"Pattus","sequence":"additional","affiliation":[{"name":"De\u0301partement Re\u0301cepteurs et Prote\u0301ines Membranaires"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"6391","DOI":"10.1021\/ic00104a059","volume":"33","year":"1994","unstructured":"Albrecht-Gary, A. 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