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This study used a label-free shotgun proteomics method to determine the\n            <jats:italic>F. tularensis<\/jats:italic>\n            subsp.\n            <jats:italic>novicida<\/jats:italic>\n            (\n            <jats:italic>F. novicida<\/jats:italic>\n            ) proteins that are regulated by MglA. The differences in relative protein amounts between wild-type\n            <jats:italic>F. novicida<\/jats:italic>\n            and the\n            <jats:italic>mglA<\/jats:italic>\n            mutant were derived directly from the average peptide precursor ion intensity values measured with the mass spectrometer by using a suite of mathematical algorithms. Among the proteins whose relative amounts changed in an\n            <jats:italic>F. novicida mglA<\/jats:italic>\n            mutant were homologs of oxidative and general stress response proteins. The\n            <jats:italic>F. novicida mglA<\/jats:italic>\n            mutant exhibited decreased survival during stationary-phase growth and increased susceptibility to killing by superoxide generated by the redox-cycling agent paraquat. The\n            <jats:italic>F. novicida mglA<\/jats:italic>\n            mutant also showed increased survival upon exposure to hydrogen peroxide, likely due to increased amounts of the catalase KatG. Our results suggested that MglA coordinates the stress response of\n            <jats:italic>F. tularensis<\/jats:italic>\n            and is likely essential for bacterial survival in harsh environments.\n          <\/jats:p>","DOI":"10.1128\/jb.00809-07","type":"journal-article","created":{"date-parts":[[2007,7,21]],"date-time":"2007-07-21T00:44:41Z","timestamp":1184978681000},"page":"6580-6586","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":55,"title":["MglA Regulates\n            <i>Francisella tularensis<\/i>\n            subsp.\n            <i>novicida<\/i>\n            (\n            <i>Francisella novicida<\/i>\n            ) Response to Starvation and Oxidative Stress"],"prefix":"10.1128","volume":"189","author":[{"given":"Tina","family":"Guina","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Division of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dragan","family":"Radulovic","sequence":"additional","affiliation":[{"name":"Department of Mathematics, Florida Atlantic University, Boca Raton, Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arya J.","family":"Bahrami","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana L.","family":"Bolton","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Rohmer","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kendan A.","family":"Jones-Isaac","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinzy","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larry A.","family":"Gallagher","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Byron","family":"Gallis","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soyoung","family":"Ryu","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greg K.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitchell J.","family":"Brittnacher","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin","family":"Manoil","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Goodlett","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"28039","DOI":"10.1074\/jbc.M605174200","volume":"281","year":"2006","unstructured":"Bang, I. 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