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Additionally, \u03c3<jats:sup>B<\/jats:sup>can be activated by blue light via the phototropin homologue YtvA, a component of the environmental branch of the signaling cascade. Here we use a reporter-gene fusion to show that \u03c3<jats:sup>B<\/jats:sup>can also be activated by red light via the energy branch of its upstream signaling cascade. Deletion mutagenesis and homologous overproduction experiments indicate that the RsbP protein (composed of an N-terminal Per-ARNT-Sim [PAS] domain and a C-terminal PP2C-type phosphatase domain) is involved in the red light response. This second light input pathway functions complementarily to YtvA; it shows broader spectral sensitivity but requires higher light intensities. These results are confirmed by transcriptome analyses, which show that both light effects result in upregulation of the \u03c3<jats:sup>B<\/jats:sup>regulon, with minimal activation of other responses.<\/jats:p>","DOI":"10.1128\/jb.00826-09","type":"journal-article","created":{"date-parts":[[2009,12,1]],"date-time":"2009-12-01T02:34:12Z","timestamp":1259634852000},"page":"755-762","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Red Light Activates the \u03c3<sup>B<\/sup><b>-<\/b>Mediated General Stress Response of<i>Bacillus subtilis<\/i>via the Energy Branch of the Upstream Signaling Cascade"],"prefix":"10.1128","volume":"192","author":[{"given":"Marcela","family":"A\u0301vila-Pe\u0301rez","sequence":"first","affiliation":[{"name":"Laboratory for Microbiology, Swammerdam Institute for Life Sciences, NISB, University of Amsterdam, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeroen B.","family":"van der Steen","sequence":"additional","affiliation":[{"name":"Laboratory for Microbiology, Swammerdam Institute for Life Sciences, NISB, University of Amsterdam, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Remco","family":"Kort","sequence":"additional","affiliation":[{"name":"Laboratory for Microbiology, Swammerdam Institute for Life Sciences, NISB, University of Amsterdam, Amsterdam, The Netherlands"},{"name":"TNO Quality of Life, Business Unit Food and Biotechnology Innovations, Microbial Genomics Group, Utrechtseweg 48, 3704 HE Zeist, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaas J.","family":"Hellingwerf","sequence":"additional","affiliation":[{"name":"Laboratory for Microbiology, Swammerdam Institute for Life Sciences, NISB, University of Amsterdam, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01208-07"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.4.1329-1338.2001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.16.4478-4490.2000"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00716-06"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"24958","DOI":"10.1074\/jbc.M109.033316","volume":"284","year":"2009","unstructured":"\u00c1vila-P\u00e9rez, M. 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