{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T22:42:50Z","timestamp":1791153770380,"version":"4.1.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"18","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Bacteriol."],"published-print":{"date-parts":[[2012,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The organic hydroperoxide resistance protein Ohr has been identified in numerous bacteria where it functions in the detoxification of organic hydroperoxides, and expression of<jats:italic>ohr<\/jats:italic>is often regulated by a MarR-type regulator called OhrR. The genes annotated as BAB2_0350 and BAB2_0351 in the<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella abortus<\/jats:named-content>2308 genome sequence are predicted to encode OhrR and Ohr orthologs, respectively. Using isogenic<jats:italic>ohr<\/jats:italic>and<jats:italic>ohrR<\/jats:italic>mutants and<jats:italic>lacZ<\/jats:italic>promoter fusions, it was determined that Ohr contributes to resistance to organic hydroperoxide, but not hydrogen peroxide, in<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. abortus<\/jats:named-content>2308 and that OhrR represses the transcription of both<jats:italic>ohr<\/jats:italic>and<jats:italic>ohrR<\/jats:italic>in this strain. Moreover, electrophoretic mobility shift assays and DNase I footprinting revealed that OhrR binds directly to a specific region in the intergenic region between<jats:italic>ohr<\/jats:italic>and<jats:italic>ohrR<\/jats:italic>that shares extensive nucleotide sequence similarity with so-called \u201cOhrR boxes\u201d described in other bacteria. While Ohr plays a prominent role in protecting<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. abortus<\/jats:named-content>2308 from organic hydroperoxide stress in<jats:italic>in vitro<\/jats:italic>assays, this protein is not required for the wild-type virulence of this strain in cultured murine macrophages or experimentally infected mice.<\/jats:p>","DOI":"10.1128\/jb.00873-12","type":"journal-article","created":{"date-parts":[[2012,7,22]],"date-time":"2012-07-22T00:14:00Z","timestamp":1342916040000},"page":"5065-5072","source":"Crossref","is-referenced-by-count":21,"title":["Characterization of the Organic Hydroperoxide Resistance System of Brucella abortus 2308"],"prefix":"10.1128","volume":"194","author":[{"given":"Clayton C.","family":"Caswell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John E.","family":"Baumgartner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel W.","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"II","given":"R. 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