{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T23:02:57Z","timestamp":1791414177134,"version":"4.3.3"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Bacteriology"],"published-print":{"date-parts":[[2014,3,1]]},"DOI":"10.1128\/jb.00993-13","type":"journal-article","created":{"date-parts":[[2013,12,21]],"date-time":"2013-12-21T05:43:57Z","timestamp":1387604637000},"page":"1020-1030","source":"Crossref","is-referenced-by-count":38,"title":["The LuxR-Type Regulator VpsT Negatively Controls the Transcription of rpoS, Encoding the General Stress Response Regulator, in Vibrio cholerae Biofilms"],"prefix":"10.1128","volume":"196","author":[{"given":"H.","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. C.","family":"Ayala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. A.","family":"Benitez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. J.","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","published-online":{"date-parts":[[2013,12,20]]},"reference":[{"key":"key20180919081334_B1","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1128\/MMBR.66.3.373-395.2002","article-title":"Signal transduction and regulatory mechanisms involved in control of the sigma S (RpoS) subunit of RNA polymerase","volume":"66","author":"Hengge-Aronis","year":"2002","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"key20180919081334_B2","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1128\/JB.180.4.773-784.1998","article-title":"Role of rpoS in stress survival and virulence of Vibrio cholerae","volume":"180","author":"Yildiz","year":"1998","journal-title":"J. Bacteriol."},{"key":"key20180919081334_B3","doi-asserted-by":"publisher","first-page":"6350","DOI":"10.1073\/pnas.0601277103","article-title":"Transmissibility of cholera: in vivo formed biofilms and their relationship to infectivity and persistence in the environment","volume":"103","author":"Faruque","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"key20180919081334_B4","doi-asserted-by":"publisher","first-page":"1141","DOI":"10.1128\/IAI.74.2.1141-1147.2006","article-title":"Genetic and phenotypic diversity of quorum-sensing systems in clinical and environmental isolates of Vibrio cholerae","volume":"74","author":"Joelsson","year":"2006","journal-title":"Infect. Immun."},{"key":"key20180919081334_B5","doi-asserted-by":"publisher","first-page":"16819","DOI":"10.1073\/pnas.0505350102","article-title":"Biofilm formation and phenotypic variation enhance predation-driven persistence of Vibrio cholerae","volume":"102","author":"Matz","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"key20180919081334_B6","doi-asserted-by":"publisher","first-page":"REVIEWS1016.1\u2013REVIEWS1016.3","DOI":"10.1186\/gb-2000-1-3-reviews1016","article-title":"The complete genome sequence of Vibrio cholerae: a tale of two chromosomes and two lifestyles","volume":"1","author":"Schoolnik","year":"2000","journal-title":"Genome Biol."},{"key":"key20180919081334_B7","doi-asserted-by":"publisher","first-page":"4028","DOI":"10.1073\/pnas.96.7.4028","article-title":"Vibrio cholerae O1 El Tor: identification of a gene cluster required for the rugose colony type, exopolysaccharide production, chlorine resistance, and biofilm formation","volume":"96","author":"Yildiz","year":"1999","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"key20180919081334_B8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1002\/path.1700460102","article-title":"The rugose variant of vibrios","volume":"46","author":"White","year":"1938","journal-title":"J. Pathol. Bacteriol."},{"key":"key20180919081334_B9","doi-asserted-by":"publisher","first-page":"1364","DOI":"10.1093\/infdis\/174.6.1364","article-title":"Vibrio cholerae O1 can assume a chlorine-resistant rugose survival form that is virulent for humans","volume":"174","author":"Morris","year":"1996","journal-title":"J. Infect. Dis."},{"key":"key20180919081334_B10","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1016\/0140-6736(92)92289-R","article-title":"Chlorine and survival of \u201crugose\u201d Vibrio cholerae","volume":"340","author":"Rice","year":"1992","journal-title":"Lancet"},{"key":"key20180919081334_B11","doi-asserted-by":"crossref","first-page":"3648","DOI":"10.1128\/AEM.64.10.3648-3655.1998","article-title":"Vibrio cholerae O1 strain TSI-4 produces the exopolysaccharide materials that determine colony morphology, stress resistance, and biofilm formation","volume":"64","author":"Wai","year":"1998","journal-title":"Appl. Environ. Microbiol."},{"key":"key20180919081334_B12","doi-asserted-by":"publisher","first-page":"2497","DOI":"10.1099\/mic.0.27099-0","article-title":"Cyclic di-GMP as a bacterial second messenger","volume":"150","author":"D'Argenio","year":"2004","journal-title":"Microbiology"},{"key":"key20180919081334_B13","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1038\/nrmicro2109","article-title":"Principles of c-di-GMP signaling in bacteria","volume":"7","author":"Hengge","year":"2009","journal-title":"Nat. Rev. Microbiol."},{"key":"key20180919081334_B14","doi-asserted-by":"publisher","first-page":"1123","DOI":"10.1111\/j.1365-2958.2004.04206.x","article-title":"GGDEF and EAL domains inversely regulate cyclic di-GMP levels and transition from sessility to motility","volume":"53","author":"Simm","year":"2004","journal-title":"Mol. Microbiol."},{"key":"key20180919081334_B15","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1146\/annurev.micro.61.080706.093426","article-title":"Roles of cyclic diguanylate in the regulation of bacterial pathogenesis","volume":"61","author":"Tamayo","year":"2007","journal-title":"Annu. Rev. Microbiol."},{"key":"key20180919081334_B16","doi-asserted-by":"publisher","first-page":"1716","DOI":"10.1128\/JB.183.5.1716-1726.2001","article-title":"VpsR, a member of the response regulators of the two-component regulatory systems, is required for expression of vps biosynthesis genes and EPS (ETr)-associated phenotypes in Vibrio cholerae O1 El Tor","volume":"183","author":"Yildiz","year":"2001","journal-title":"J. Bacteriol."},{"key":"key20180919081334_B17","doi-asserted-by":"publisher","first-page":"1574","DOI":"10.1128\/JB.186.5.1574-1578.2004","article-title":"VpsT is a transcriptional regulator required for expression of vps biosynthesis genes and the development of rugose colonial morphology in Vibrio cholerae O1 El Tor","volume":"186","author":"Casper-Lindley","year":"2004","journal-title":"J. Bacteriol."},{"key":"key20180919081334_B18","doi-asserted-by":"publisher","first-page":"6331","DOI":"10.1128\/JB.05167-11","article-title":"Integration of cyclic di-GMP and quorum sensing in the control of vpsT and aphA in Vibrio cholerae","volume":"193","author":"Srivastava","year":"2011","journal-title":"J. Bacteriol."},{"key":"key20180919081334_B19","doi-asserted-by":"publisher","first-page":"866","DOI":"10.1126\/science.1181185","article-title":"Vibrio cholerae VpsT regulates matrix production and motility by directly sensing cyclic di-GMP","volume":"327","author":"Krasteva","year":"2010","journal-title":"Science"},{"key":"key20180919081334_B20","doi-asserted-by":"publisher","first-page":"2527","DOI":"10.1128\/JB.01756-07","article-title":"Quorum sensing controls biofilm formation in Vibrio cholerae through modulation of cyclic di-GMP levels and repression of vpsT","volume":"190","author":"Waters","year":"2008","journal-title":"J. Bacteriol."},{"key":"key20180919081334_B21","doi-asserted-by":"publisher","first-page":"3742","DOI":"10.1128\/AEM.02804-06","article-title":"Quorum sensing enhances the stress response in Vibrio cholerae","volume":"73","author":"Joelsson","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"key20180919081334_B22","doi-asserted-by":"publisher","first-page":"6529","DOI":"10.1128\/JB.05166-11","article-title":"Interplay among cyclic diguanylate, HapR, and the general stress response regulator (RpoS) in the regulation of Vibrio cholerae hemagglutinin\/protease","volume":"193","author":"Wang","year":"2011","journal-title":"J. Bacteriol."},{"key":"key20180919081334_B23","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/0378-1119(93)90533-9","article-title":"Analysis of the Pseudomonas gene products using lacIq\/Ptrp-lac plasmids and transposons that confer conditional phenotypes","volume":"123","author":"De Lorenzo","year":"1993","journal-title":"Gene"},{"key":"key20180919081334_B24","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1111\/j.1574-6968.1990.tb04139.x","article-title":"Effect of DNase production, plasmid size and restriction barrier on transformation of Vibrio cholerae by electroporation and osmotic shock","volume":"68","author":"Marcus","year":"1990","journal-title":"FEMS Microbiol. Lett."},{"key":"key20180919081334_B25","doi-asserted-by":"publisher","first-page":"857","DOI":"10.1111\/j.1365-2958.2004.04155.x","article-title":"Cyclic diguanylate (c-di-GMP) regulates Vibrio cholerae biofilm formation","volume":"53","author":"Tischler","year":"2004","journal-title":"Mol. Microbiol."},{"key":"key20180919081334_B26","doi-asserted-by":"crossref","first-page":"4310","DOI":"10.1128\/IAI.59.12.4310-4317.1991","article-title":"Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive selection suicide vector","volume":"59","author":"Donnenberg","year":"1991","journal-title":"Infect. Immun."},{"key":"key20180919081334_B27","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/S0378-1119(96)00778-0","article-title":"Suicide plasmids containing promoterless reporter genes can simultaneously disrupt and create fusions to target genes of diverse bacteria","volume":"188","author":"Kalogeraki","year":"1997","journal-title":"Gene"},{"key":"key20180919081334_B28","doi-asserted-by":"publisher","first-page":"1883","DOI":"10.1099\/mic.0.26086-0","article-title":"Haemagglutinin\/protease expression and mucin gel penetration in El Tor biotype Vibrio cholerae","volume":"149","author":"Silva","year":"2003","journal-title":"Microbiology"},{"key":"key20180919081334_B29","doi-asserted-by":"publisher","first-page":"6374","DOI":"10.1128\/JB.186.19.6374-6382.2004","article-title":"Transcriptional regulation of Vibrio cholerae hemagglutinin\/protease by the cyclic AMP receptor protein and RpoS","volume":"186","author":"Silva","year":"2004","journal-title":"J. Bacteriol."},{"key":"key20180919081334_B30","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1016\/S0092-8674(02)00829-2","article-title":"Parallel quorum sensing systems converge to regulate virulence in Vibrio cholerae","volume":"110","author":"Miller","year":"2002","journal-title":"Cell"},{"key":"key20180919081334_B31","doi-asserted-by":"publisher","first-page":"2482","DOI":"10.1128\/AEM.07629-11","article-title":"The histone-like nucleoid structuring protein (H-NS) is a repressor of Vibrio cholerae exopolysaccharide biosynthesis (vps) genes","volume":"78","author":"Wang","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"key20180919081334_B32","doi-asserted-by":"publisher","first-page":"1205","DOI":"10.1128\/JB.05900-11","article-title":"Interaction of the histone-like nucleoid structuring protein and the general stress response regulator RpoS at Vibrio cholerae promoters that regulate motility and hemagglutinin\/protease expression","volume":"194","author":"Wang","year":"2012","journal-title":"J. Bacteriol."},{"key":"key20180919081334_B33","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1002\/bmb.20649","article-title":"Using electrophoretic mobility shift assays to measure equilibrium dissociation constants: Gal4-p53 binding DNA as a model system","volume":"40","author":"Heffler","year":"2012","journal-title":"Biochem. Mol. Biol. Educ."},{"key":"key20180919081334_B34","first-page":"103","article-title":"Identification of the DNA bases of a DNase I footprint by the use of dye primer sequencing on an automated capillary DNA analysis instrument","volume":"17","author":"Zianni","year":"2006","journal-title":"J. Biomol. Tech."},{"key":"key20180919081334_B35","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1016\/j.mimet.2004.10.009","article-title":"Identifying cloned Helicobacter pylori promoters by primer extension using a FAM-labelled primer and GeneScan analysis","volume":"60","author":"Lloyd","year":"2005","journal-title":"J. Microbiol. Methods"},{"key":"key20180919081334_B36","volume-title":"Experiments in molecular genetics","author":"Miller","year":"1971"},{"key":"key20180919081334_B37","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.mib.2006.12.006","article-title":"c-di-GMP-mediated regulation of virulence and biofilm formation","volume":"10","author":"Cotter","year":"2007","journal-title":"Curr. Opin. Microbiol."},{"key":"key20180919081334_B38","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/j.tim.2008.12.004","article-title":"Vibrio biofilms: so much the same yet so different","volume":"17","author":"Yildiz","year":"2009","journal-title":"Trends Microbiol."},{"key":"key20180919081334_B39","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1016\/j.mib.2011.12.008","article-title":"Mechanism of c-di-GMP signaling","volume":"15","author":"Sondermann","year":"2012","journal-title":"Curr. Opin. Microbiol."},{"key":"key20180919081334_B40","doi-asserted-by":"publisher","first-page":"5233","DOI":"10.1128\/AEM.01596-13","article-title":"Exploring environmental control of c-di-GMP signaling in Vibrio cholerae by using the ex vivo lysate cyclic di-GMP assay (TELCA)","volume":"79","author":"Koestler","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"key20180919081334_B41","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1146\/annurev-micro-090110-102946","article-title":"The RpoS-mediated general stress response in Escherichia coli","volume":"65","author":"Battesti","year":"2011","journal-title":"Annu. Rev. Microbiol."},{"key":"key20180919081334_B42","doi-asserted-by":"publisher","first-page":"1137","DOI":"10.1046\/j.1365-2958.1996.00063.x","article-title":"A hierarchical quorum-sensing cascade in Pseudomonas aeruginosa links transcriptional activators LasR and RhlR (VsmR) to expression of the stationary-phase sigma factor RpoS","volume":"21","author":"Latifi","year":"1996","journal-title":"Mol. Microbiol."},{"key":"key20180919081334_B43","doi-asserted-by":"publisher","first-page":"805","DOI":"10.1128\/JB.182.3.805-811.2000","article-title":"Conversion of the Vibrio fischeri transcriptional activator, LuxR, to a repressor","volume":"182","author":"Egland","year":"2000","journal-title":"J. Bacteriol."},{"key":"key20180919081334_B44","doi-asserted-by":"publisher","first-page":"5683","DOI":"10.1128\/JB.01807-06","article-title":"Crystal structure of the Vibrio cholerae quorum-sensing regulatory protein HapR","volume":"189","author":"De Silva","year":"2007","journal-title":"J. Bacteriol."},{"key":"key20180919081334_B45","doi-asserted-by":"publisher","first-page":"1485","DOI":"10.1128\/JB.185.5.1485-1491.2003","article-title":"Diffusion in biofilms","volume":"185","author":"Stewart","year":"2003","journal-title":"J. Bacteriol."},{"key":"key20180919081334_B46","doi-asserted-by":"publisher","first-page":"1462","DOI":"10.1002\/bit.21695","article-title":"Significance of rpoS during maturation of Escherichia coli biofilms","volume":"99","author":"Ito","year":"2008","journal-title":"Biotechnol. Bioeng."},{"key":"key20180919081334_B47","doi-asserted-by":"publisher","first-page":"975","DOI":"10.1002\/bit.22305","article-title":"Localized expression profiles of rpoS in Escherichia coli biofilms","volume":"103","author":"Ito","year":"2009","journal-title":"Biotechnol. Bioeng."},{"key":"key20180919081334_B48","doi-asserted-by":"publisher","first-page":"7335","DOI":"10.1128\/JB.00360-08","article-title":"Role of the histone-like nucleoid structuring protein (H-NS) in the regulation of RpoS and RpoS-dependent genes in Vibrio cholerae","volume":"190","author":"Silva","year":"2008","journal-title":"J. Bacteriol."},{"key":"key20180919081334_B49","doi-asserted-by":"publisher","first-page":"e109","DOI":"10.1371\/journal.ppat.0020109","article-title":"RpoS controls the Vibrio cholerae mucosal escape response","volume":"2","author":"Nielsen","year":"2006","journal-title":"PLoS Pathog."},{"key":"key20180919081334_B50","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1111\/j.1365-2958.2004.04000.x","article-title":"Genetic evidence that the Vibrio cholerae monolayer is a distinct stage in biofilm development","volume":"52","author":"Moorthy","year":"2004","journal-title":"Mol. Microbiol."},{"key":"key20180919081334_B51","doi-asserted-by":"publisher","first-page":"2675","DOI":"10.1128\/JB.182.10.2675-2679.2000","article-title":"Biofilm, city of microbes","volume":"182","author":"Watnick","year":"2000","journal-title":"J. Bacteriol."},{"key":"key20180919081334_B52","doi-asserted-by":"publisher","first-page":"2964","DOI":"10.1099\/mic.0.2007\/006668-0","article-title":"The cyclic AMP receptor protein modulates quorum sensing, motility and multiple genes that affect intestinal colonization in Vibrio cholerae","volume":"153","author":"Liang","year":"2007","journal-title":"Microbiology"},{"key":"key20180919081334_B53","doi-asserted-by":"crossref","first-page":"4717","DOI":"10.1128\/jb.173.15.4717-4724.1991","article-title":"Functional analysis of the Pseudomonas putida regulatory protein CatR: transcriptional studies and determination of the CatR DNA binding site by hydroxyl-radical footprinting","volume":"173","author":"Rothmel","year":"1991","journal-title":"J. Bacteriol."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1128\/JB.00993-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:37:19Z","timestamp":1627580239000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.00993-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12,20]]},"references-count":53,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2014,2,6]]},"published-print":{"date-parts":[[2014,3,1]]}},"URL":"https:\/\/doi.org\/10.1128\/jb.00993-13","relation":{},"ISSN":["0021-9193"],"issn-type":[{"value":"0021-9193","type":"print"}],"subject":[],"published":{"date-parts":[[2013,12,20]]}}}