{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T02:54:12Z","timestamp":1792032852825,"version":"4.4.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"22","license":[{"start":{"date-parts":[[2006,11,15]],"date-time":"2006-11-15T00:00:00Z","timestamp":1163548800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2006,11,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The exosporium-defective phenotype of a transposon insertion mutant of\n            <jats:italic>Bacillus cereus<\/jats:italic>\n            implicated ExsY, a homologue of\n            <jats:italic>B. subtilis<\/jats:italic>\n            cysteine-rich spore coat proteins CotY and CotZ, in assembly of an intact exosporium. Single and double mutants of\n            <jats:italic>B. cereus<\/jats:italic>\n            lacking ExsY and its paralogue, CotY, were constructed. The\n            <jats:italic>exsY<\/jats:italic>\n            mutant spores are not surrounded by an intact exosporium, though they often carry attached exosporium fragments. In contrast, the\n            <jats:italic>cotY<\/jats:italic>\n            mutant spores have an intact exosporium, although its overall shape is altered. The single mutants show altered, but different, spore coat properties. The\n            <jats:italic>exsY<\/jats:italic>\n            mutant spore coat is permeable to lysozyme, whereas the\n            <jats:italic>cotY<\/jats:italic>\n            mutant spores are less resistant to several organic solvents than is the case for the wild type. The\n            <jats:italic>exsY cotY<\/jats:italic>\n            double-mutant spores lack exosporium and have very thin coats that are permeable to lysozyme and are sensitive to chloroform, toluene, and phenol. These spore coat as well as exosporium defects suggest that ExsY and CotY are important to correct formation of both the exosporium and the spore coat in\n            <jats:italic>B. cereus.<\/jats:italic>\n            Both ExsY and CotY proteins were detected in Western blots of purified wild-type exosporium, in complexes of high molecular weight, and as monomers. Both\n            <jats:italic>exsY<\/jats:italic>\n            and\n            <jats:italic>cotY<\/jats:italic>\n            genes are expressed at late stages of sporulation.\n          <\/jats:p>","DOI":"10.1128\/jb.00997-06","type":"journal-article","created":{"date-parts":[[2006,11,2]],"date-time":"2006-11-02T18:24:30Z","timestamp":1162491870000},"page":"7905-7913","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":63,"title":["ExsY and CotY Are Required for the Correct Assembly of the Exosporium and Spore Coat of\n            <i>Bacillus cereus<\/i>"],"prefix":"10.1128","volume":"188","author":[{"given":"Matt J.","family":"Johnson","sequence":"first","affiliation":[{"name":"Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah J.","family":"Todd","sequence":"additional","affiliation":[{"name":"Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Ball","sequence":"additional","affiliation":[{"name":"Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew M.","family":"Shepherd","sequence":"additional","affiliation":[{"name":"Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Sylvestre","sequence":"additional","affiliation":[{"name":"Unite\u0301 Toxine et Pathoge\u0301nie Bacte\u0301riennes, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Moir","sequence":"additional","affiliation":[{"name":"Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.11.3800-3806.2005"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1111\/j.1574-6968.1989.tb03039.x","volume":"58","year":"1989","unstructured":"Bone, E. J., and D. J. Ellar. 1989. Transformation of Bacillus thuringiensis by electroporation. FEMS Lett. 58 : 171-178.","journal-title":"FEMS Lett."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.4.1781-1786.2000"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.7.3738-3744.1990"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1046\/j.1365-2672.1999.00878.x","volume":"87","year":"1999","unstructured":"Charlton, S., A. J. G. Moir, L. Baillie, and A. Moir. 1999. Characterization of the exosporium of Bacillus cereus. J. Appl. Microbiol. 87 : 241-245.","journal-title":"J. Appl. Microbiol."},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"3580","DOI":"10.1093\/nar\/16.8.3580","volume":"16","year":"1988","unstructured":"Chung, C. T., and R. H. Miller. 1988. A rapid and convenient method for the preparation and storage of competent bacterial cells. Nucleic Acids Res. 16 : 3580.","journal-title":"Nucleic Acids Res."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.24.6729-6735.1998"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"6127","DOI":"10.1093\/nar\/16.13.6127","volume":"16","year":"1988","unstructured":"Dower, W. J., J. F. Miller, and C. W. Ragsdale. 1988. High efficiency transformation of Escherichia coli by high voltage electroporation. Nucleic Acids Res. 16 : 6127-6145.","journal-title":"Nucleic Acids Res."},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1038\/nrmicro750","volume":"1","year":"2003","unstructured":"Errington, J. 2003. Regulation of endospore formation in Bacillus subtilis. Nat. Rev. Microbiol. 1 : 117-126.","journal-title":"Nat. Rev. Microbiol."},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/BF00925710","volume":"145","year":"1995","unstructured":"Garcia-Patrone, M., and J. S. Tandecarz. 1995. A glycoprotein multimer from Bacillus thuringiensis sporangia\u2014dissociation into subunits and sugar composition. Mol. Cell Biochem. 145 : 29-37.","journal-title":"Mol. Cell Biochem."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.88.6.1774-1789.1964"},{"key":"e_1_3_2_13_2","first-page":"2421","volume":"131","year":"1985","unstructured":"James, W., and J. Mandelstam. 1985. Protease production during sporulation of germination mutants of Bacillus subtilis and the cloning of a functional gerE gene. J. Gen. Microbiol. 131 : 2421-2430.","journal-title":"J. Gen. Microbiol."},{"key":"e_1_3_2_14_2","unstructured":"Johnson M. J. 2004. The function of ExsY and CotY two structural proteins of the Bacillus cereus exosporium. Ph.D. thesis. University of Sheffield Sheffield United Kingdom."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.8.2413-2417.2004"},{"key":"e_1_3_2_16_2","first-page":"2717","volume":"135","year":"1989","unstructured":"Koshikawa, T., M. Yamazaki, M. Yoshimi, S. Ogawa, A. Yamada, K. Watabe, and M. Torii. 1989. Surface hydrophobicity of spores of Bacillus spp. J. Gen. Microbiol. 135 : 2717-2722.","journal-title":"J. Gen. Microbiol."},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"2125","DOI":"10.1099\/00221287-144-8-2125","volume":"144","year":"1998","unstructured":"Leatherbarrow, A. J. H., M. A. Yazdi, J. P. Curson, and A. Moir. 1998. The gerC locus of Bacillus subtilis, required for menaquinone biosynthesis, is concerned only indirectly with spore germination. Microbiology 144 : 2125-2130.","journal-title":"Microbiology"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.101.1.196-201.1970"},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"3357","DOI":"10.1002\/j.1460-2075.1985.tb04089.x","volume":"4","year":"1985","unstructured":"Murphy, E., L. Huwyler, and M. D. D. Bastos. 1985. Transposon Tn554\u2014complete nucleotide sequence and isolation of transposition-defective and antibiotic-sensitive mutants. EMBO J. 4 : 3357-3365.","journal-title":"EMBO J."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.115.3.1179-1190.1973"},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S0378-1097(97)00321-2","volume":"154","year":"1997","unstructured":"Okstad, O. A., A. Gronstad, T. Lindback, and A. B. Kolsto. 1997. Insertional inactivation of a Tet(K)\/Tet(L) like transporter does not eliminate tetracycline resistance in Bacillus cereus. FEMS Microbiol. Lett. 154 : 181-186.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/0003-2697(77)90043-4","volume":"83","year":"1977","unstructured":"Peterson, G. L. 1977. A simplification of the protein assay of Lowry which is more generally applicable. Anal. Biochem. 83 : 346-356.","journal-title":"Anal. Biochem."},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1099\/mic.0.26681-0","volume":"150","year":"2004","unstructured":"Redmond, C., L. W. J. Baillie, S. Hibbs, A. J. G. Moir, and A. Moir. 2004. Identification of proteins in the exosporium of Bacillus anthracis. Microbiology 150 : 355-363.","journal-title":"Microbiology"},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1038\/263053a0","volume":"263","year":"1976","unstructured":"Sousa, J. C. F., M. T. Silva, and G. Balassa. 1976. An exosporium-like outer layer in Bacillus subtilis spores. Nature 263 : 53-54.","journal-title":"Nature"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.6.1903-1910.2003"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.17.5868-5876.2005"},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1042\/bj1130029","volume":"113","year":"1969","unstructured":"Sterlini, J. M., and J. Mandelstam. 1969. Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. Biochem. J. 113 : 29-37.","journal-title":"Biochem. J."},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1046\/j.1365-2958.2000.03000.x","volume":"45","year":"2002","unstructured":"Sylvestre, P., E. Couture-Tosi, and M. Mock. 2002. A collagen-like surface glycoprotein is a structural component of the Bacillus anthracis exosporium. Mol. Microbiol. 45 : 169-178.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.15.5122-5128.2005"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.5.1555-1563.2003"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.2.7.657-662.1968"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.11.3373-3378.2003"},{"key":"e_1_3_2_33_2","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.1574-6968.1987.tb02558.x","volume":"48","year":"1987","unstructured":"Trieu-Cuot, P., C. Carlier, P. Martin, and P. Courvalin. 1987. Plasmid transfer by conjugation from Escherichia coli to Gram-positive bacteria. FEMS Microbiol. Lett. 48 : 289-294.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_34_2","doi-asserted-by":"crossref","first-page":"4296","DOI":"10.1093\/nar\/18.14.4296","volume":"18","year":"1990","unstructured":"Trieu-Cuot, P., C. Carlier, C. Poyart-Salmeron, and P. Courvalin. 1990. A pair of mobilizable shuttle vectors conferring resistance to spectinomycin for molecular cloning in Escherichia coli and in Gram-positive bacteria. Nucleic Acids Res. 18 : 4296.","journal-title":"Nucleic Acids Res."},{"key":"e_1_3_2_35_2","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/0378-1119(91)90546-N","volume":"102","year":"1991","unstructured":"Trieu-Cuot, P., C. Carlier, C. Poyart-Salmeron, and P. Courvalin. 1991. Shuttle vectors containing a multiple cloning site and a lacZ alpha gene for conjugal transfer of DNA from Escherichia coli to Gram-positive bacteria. Gene 102 : 99-104.","journal-title":"Gene"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(85)90120-9"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.102.2.573-579.1970"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.12.3757-3766.1993"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00997-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00997-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:31:21Z","timestamp":1627579881000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.00997-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,11,15]]},"references-count":37,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2006,11,15]]}},"alternative-id":["10.1128\/JB.00997-06"],"URL":"https:\/\/doi.org\/10.1128\/jb.00997-06","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,11,15]]},"assertion":[{"value":"2006-07-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-09-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}