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LqsR promotes host-cell interactions as an element of the stationary-phase virulence regulatory network. Here, we characterize<jats:italic>L. pneumophila<\/jats:italic>mutant strains lacking all four genes of the<jats:italic>lqs<\/jats:italic>cluster or only the<jats:italic>hdeD<\/jats:italic>gene. While an<jats:italic>hdeD<\/jats:italic>mutant strain did not have overt physiological or virulence phenotypes, an<jats:italic>lqs<\/jats:italic>mutant showed an aberrant morphology in stationary growth phase and was defective for intracellular growth, efficient phagocytosis, and cytotoxicity against host cells. Cytotoxicity was restored upon reintroduction of the<jats:italic>lqs<\/jats:italic>genes into the chromosome of an<jats:italic>lqs<\/jats:italic>mutant strain. The deletion of the<jats:italic>lqs<\/jats:italic>cluster caused more-severe phenotypes than deletion of only<jats:italic>lqsR<\/jats:italic>, suggesting a synergistic effect of the other<jats:italic>lqs<\/jats:italic>genes. A transcriptome analysis indicated that in the stationary phase more than 380 genes were differentially regulated in the<jats:italic>lqs<\/jats:italic>mutant and wild-type<jats:italic>L. pneumophila<\/jats:italic>. Genes involved in protein production, metabolism, and bioenergetics were upregulated in the<jats:italic>lqs<\/jats:italic>mutant, whereas genes encoding virulence factors, such as effectors secreted by the Icm\/Dot type IV secretion system, were downregulated. A proteome analysis revealed that a set of Icm\/Dot substrates is not produced in the absence of the<jats:italic>lqs<\/jats:italic>gene cluster, which confirms the findings from DNA microarray assays and mirrors the virulence phenotype of the<jats:italic>lqs<\/jats:italic>mutant strain.<\/jats:p>","DOI":"10.1128\/jb.01002-08","type":"journal-article","created":{"date-parts":[[2008,9,20]],"date-time":"2008-09-20T01:07:25Z","timestamp":1221872845000},"page":"7532-7547","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":77,"title":["Synergistic Contribution of the<i>Legionella pneumophila lqs<\/i>Genes to Pathogen-Host Interactions"],"prefix":"10.1128","volume":"190","author":[{"given":"Andre\u0301","family":"Tiaden","sequence":"first","affiliation":[{"name":"Institute of Microbiology, ETH Zu\u0308rich, Wolfgang-Pauli-Strasse 10, 8093 Zu\u0308rich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Spirig","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, ETH Zu\u0308rich, Wolfgang-Pauli-Strasse 10, 8093 Zu\u0308rich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Carranza","sequence":"additional","affiliation":[{"name":"Institute of Plant Biology, University of Zu\u0308rich, Zollikerstr. 10, 8008 Zu\u0308rich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger","family":"Bru\u0308ggemann","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Biologie des Bacte\u0301ries Intracellulaires and CNRS URA 2171, Institut Pasteur, 28 Rue du Dr Roux, 75724 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathrin","family":"Riedel","sequence":"additional","affiliation":[{"name":"Institute of Plant Biology, University of Zu\u0308rich, Zollikerstr. 10, 8008 Zu\u0308rich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leo","family":"Eberl","sequence":"additional","affiliation":[{"name":"Institute of Plant Biology, University of Zu\u0308rich, Zollikerstr. 10, 8008 Zu\u0308rich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Buchrieser","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Biologie des Bacte\u0301ries Intracellulaires and CNRS URA 2171, Institut Pasteur, 28 Rue du Dr Roux, 75724 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hubert","family":"Hilbi","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, ETH Zu\u0308rich, Wolfgang-Pauli-Strasse 10, 8093 Zu\u0308rich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1099\/mic.0.27563-0","volume":"151","year":"2005","unstructured":"Albers, U., K. 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