{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T13:17:14Z","timestamp":1789996634932,"version":"4.0.1"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"23","license":[{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Type IV secretion systems (T4SS) are multiprotein structures that direct the translocation of specific molecules across the bacterial cell envelope. As in other bacteria, pathogenicity of the genus\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella<\/jats:named-content>\n            essentially depends on the integrity of the T4SS-encoding\n            <jats:italic>virB<\/jats:italic>\n            operon, whose expression is regulated by multiple transcription factors belonging to different families. Previously, we identified IHF and HutC, two direct regulators of the\n            <jats:italic>virB<\/jats:italic>\n            genes that were isolated from total protein extracts of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella<\/jats:named-content>\n            . Here, we report the identification of MdrA, a third regulatory element that was isolated using the same screening procedure. This transcription factor, which belongs to the MarR-family of transcriptional regulators, binds at two different sites of the\n            <jats:italic>virB<\/jats:italic>\n            promoter and regulates expression in a growth phase-dependent manner. Like other members of the MarR family, specific ligands were able to dissociate MdrA from DNA\n            <jats:italic>in vitro<\/jats:italic>\n            . Determination of the MdrA-binding sites by DNase I footprinting and analyses of protein-DNA complexes by electrophoresis mobility shift assays (EMSAs) showed that MdrA competes with IHF and HutC for the binding to the promoter because their target DNA sequences overlap. Unlike IHF, both MdrA and HutC bound to the promoter without inducing bending of DNA. Moreover, the two latter transcription factors activated\n            <jats:italic>virB<\/jats:italic>\n            expression to similar extents, and in doing so, they are functionally redundant. Taken together, our results show that MdrA is a regulatory element that directly modulates the activity of the\n            <jats:italic>virB<\/jats:italic>\n            promoter and is probably involved in coordinating gene expression in response to specific environmental signals.\n          <\/jats:p>","DOI":"10.1128\/jb.01007-12","type":"journal-article","created":{"date-parts":[[2012,9,23]],"date-time":"2012-09-23T00:13:53Z","timestamp":1348359233000},"page":"6431-6440","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["A MarR-Type Regulator Directly Activates Transcription from the Brucella abortus virB Promoter by Sharing a Redundant Role with HutC"],"prefix":"10.1128","volume":"194","author":[{"given":"Rodrigo","family":"Sieira","sequence":"first","affiliation":[{"name":"Fundaci\u00f3n Instituto Leloir, IIBBA-CONICET, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gast\u00f3n M.","family":"Arocena","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Instituto Leloir, IIBBA-CONICET, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angeles","family":"Zorreguieta","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Instituto Leloir, IIBBA-CONICET, Buenos Aires, Argentina"},{"name":"Facultad de Ciencias Exactas y Naturales-Departamento de Qu\u00edmica Biol\u00f3gica, Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego J.","family":"Comerci","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnol\u00f3gicas, Universidad Nacional de General San Mart\u00edn, IIB-INTECH-CONICET, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo A.","family":"Ugalde","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnol\u00f3gicas, Universidad Nacional de General San Mart\u00edn, IIB-INTECH-CONICET, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1038\/90429","article-title":"The crystal structure of MarR, a regulator of multiple antibiotic resistance, at 2.3 A resolution","volume":"8","author":"Alekshun MN","year":"2001","unstructured":"AlekshunMN LevySB MealyTR SeatonBA HeadJF. 2001. 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