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We show here that the<jats:italic>B. anthracis i<\/jats:italic>ron-regulated<jats:italic>s<\/jats:italic>urface<jats:italic>d<\/jats:italic>eterminants (<jats:italic>isd<\/jats:italic>) locus encompasses<jats:italic>isdC<\/jats:italic>, specifying a heme-iron binding surface protein. Anchoring of IsdC to the cell wall envelopes of vegetative bacilli requires<jats:italic>srtB<\/jats:italic>, which encodes sortase B. Purified sortase B cleaves IsdC between the threonine and the glycine of its NPKTG motif sorting signal.<jats:italic>B. anthracis<\/jats:italic>variants lacking either<jats:italic>isdC<\/jats:italic>or<jats:italic>srtB<\/jats:italic>display defects in heme-iron scavenging, suggesting that IsdC binding to heme-iron in the cell wall envelope contributes to bacterial uptake of heme.<\/jats:p>","DOI":"10.1128\/jb.01011-06","type":"journal-article","created":{"date-parts":[[2006,11,16]],"date-time":"2006-11-16T03:29:02Z","timestamp":1163647742000},"page":"8145-8152","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":126,"title":["Surface Protein IsdC and Sortase B Are Required for Heme-Iron Scavenging of<i>Bacillus anthracis<\/i>"],"prefix":"10.1128","volume":"188","author":[{"given":"Anthony W.","family":"Maresso","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, Illinois 60637"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Travis J.","family":"Chapa","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, Illinois 60637"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olaf","family":"Schneewind","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, Illinois 60637"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","unstructured":"Andrade M. 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