{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T11:56:47Z","timestamp":1791979007536,"version":"4.4.0"},"reference-count":56,"publisher":"American Society for Microbiology","issue":"24","license":[{"start":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T00:00:00Z","timestamp":1229299200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2008,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The genomes of two closely related thermophilic cyanobacterial isolates, designated\n            <jats:italic>Synechococcus<\/jats:italic>\n            isolate OS-A and\n            <jats:italic>Synechococcus<\/jats:italic>\n            isolate OS-B\u2032, from the microbial mats of Octopus Spring (Yellowstone National Park) have been sequenced. An extensive suite of genes that are controlled by phosphate levels constitute the putative Pho regulon in these cyanobacteria. We examined physiological responses of an axenic OS-B\u2032 isolate as well as transcript abundances of Pho regulon genes as the cells acclimated to phosphorus-limiting conditions. Upon imposition of phosphorus deprivation, OS-B\u2032 stopped dividing after three to four doublings, and absorbance spectra measurements indicated that the cells had lost most of their phycobiliproteins and chlorophyll\n            <jats:italic>a<\/jats:italic>\n            . Alkaline phosphatase activity peaked and remained high after 48 h of phosphorus starvation, and there was an accumulation of transcripts from putative Pho regulon genes. Interestingly, the genome of\n            <jats:italic>Synechococcus<\/jats:italic>\n            isolate OS-B\u2032 harbors a cluster of\n            <jats:italic>phn<\/jats:italic>\n            genes that are not present in OS-A isolates. The proteins encoded by the\n            <jats:italic>phn<\/jats:italic>\n            genes function in the transport and metabolism of phosphonates, which could serve as an alternative phosphorus source when exogenous phosphate is low. The\n            <jats:italic>phn<\/jats:italic>\n            genes were upregulated within a day of eliminating the source of phosphate from the medium. However, the ability of OS-B\u2032 to utilize methylphosphonate as a sole phosphorus source occurred only after an extensive period of exposure to the substrate. Once acclimated, the cells grew rapidly in fresh medium with methylphosphonate as the only source of phosphorus. The possible implications of these results are discussed with respect to the ecophysiology of the microbial mats.\n          <\/jats:p>","DOI":"10.1128\/jb.01011-08","type":"journal-article","created":{"date-parts":[[2008,10,18]],"date-time":"2008-10-18T00:59:46Z","timestamp":1224291586000},"page":"8171-8184","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":83,"title":["Phosphorus Deprivation Responses and Phosphonate Utilization in a Thermophilic\n            <i>Synechococcus<\/i>\n            sp. from Microbial Mats"],"prefix":"10.1128","volume":"190","author":[{"given":"Melissa M.","family":"Adams","sequence":"first","affiliation":[{"name":"Carnegie Institution for Science, Department of Plant Biology, Stanford University, Stanford, California 94305"},{"name":"Department of Biology, Stanford University, Stanford, California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari\u0301a R.","family":"Go\u0301mez-Garci\u0301a","sequence":"additional","affiliation":[{"name":"Carnegie Institution for Science, Department of Plant Biology, Stanford University, Stanford, California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur R.","family":"Grossman","sequence":"additional","affiliation":[{"name":"Carnegie Institution for Science, Department of Plant Biology, Stanford University, Stanford, California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devaki","family":"Bhaya","sequence":"additional","affiliation":[{"name":"Carnegie Institution for Science, Department of Plant Biology, Stanford University, Stanford, California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.1.544-550.2006"},{"key":"e_1_3_2_3_2","unstructured":"Handbook of media for environmental microbiology. 2005"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1038\/ismej.2007.46","volume":"1","year":"2007","unstructured":"Bhaya, D., A. 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