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Acetone-induced proteins were found in extracts of cells induced by acetone, and a gene cluster designated<jats:italic>acmAB<\/jats:italic>was cloned on the basis of the N-terminal amino acid sequences of acetone-induced proteins. The<jats:italic>acmA<\/jats:italic>and<jats:italic>acmB<\/jats:italic>genes encode a Baeyer-Villiger monooxygenase (BVMO) and esterase, respectively. The BVMO encoded by<jats:italic>acmA<\/jats:italic>was purified from acetone-induced cells of<jats:italic>Gordonia<\/jats:italic>sp. strain TY-5 and characterized. The BVMO exhibited NADPH-dependent oxidation activity for linear ketones (C<jats:sub>3<\/jats:sub>to C<jats:sub>10<\/jats:sub>) and cyclic ketones (C<jats:sub>4<\/jats:sub>to C<jats:sub>8<\/jats:sub>).<jats:italic>Escherichia coli<\/jats:italic>expressing the<jats:italic>acmA<\/jats:italic>gene oxidized acetone to methyl acetate, and<jats:italic>E. coli<\/jats:italic>expressing the<jats:italic>acmB<\/jats:italic>gene hydrolyzed methyl acetate. Northern blot analyses revealed that polycistronic transcription of the<jats:italic>acmAB<\/jats:italic>gene cluster was induced by propane, 2-propanol, and acetone. These results indicate that the<jats:italic>acmAB<\/jats:italic>gene products play an important role in the metabolism of acetone derived from propane oxidation and clarify the propane metabolism pathway of strain TY-5 (propane \u2192 2-propanol \u2192 acetone \u2192 methyl acetate \u2192 acetic acid + methanol). This paper provides the first evidence for BVMO-dependent acetone metabolism.<\/jats:p>","DOI":"10.1128\/jb.01054-06","type":"journal-article","created":{"date-parts":[[2007,1,18]],"date-time":"2007-01-18T22:28:04Z","timestamp":1169159284000},"page":"886-893","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":108,"title":["Novel Acetone Metabolism in a Propane-Utilizing Bacterium,<i>Gordonia<\/i>sp. 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