{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T06:23:45Z","timestamp":1790231025704,"version":"4.1.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"24","license":[{"start":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T00:00:00Z","timestamp":1229299200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2008,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The\n            <jats:italic>Brucella<\/jats:italic>\n            cell envelope contains the zwitterionic phospholipids phosphatidylcholine (PC) and phosphatidylethanolamine (PE). Synthesis of PC occurs exclusively via the PC synthase pathway, implying that the pathogen depends on the choline synthesized by the host cell to form PC. Notably, PC is necessary to sustain a chronic infection process, which suggests that the membrane lipid content is relevant for\n            <jats:italic>Brucella<\/jats:italic>\n            virulence. In this study we investigated the first step of PE biosynthesis in\n            <jats:italic>B. abortus<\/jats:italic>\n            , which is catalyzed by phosphatidylserine synthase (PssA). Disruption of\n            <jats:italic>pssA<\/jats:italic>\n            abrogated the synthesis of PE without affecting the growth in rich complex medium. In minimal medium, however, the mutant required choline supplementation for growth, suggesting that at least PE or PC is necessary for\n            <jats:italic>Brucella<\/jats:italic>\n            viability. The absence of PE altered cell surface properties, but most importantly, it impaired several virulence traits of\n            <jats:italic>B. abortus<\/jats:italic>\n            , such as intracellular survival in both macrophages and HeLa cells, the maturation of the replicative\n            <jats:italic>Brucella<\/jats:italic>\n            -containing vacuole, and mouse colonization. These results suggest that membrane phospholipid composition is critical for the interaction of\n            <jats:italic>B. abortus<\/jats:italic>\n            with the host cell.\n          <\/jats:p>","DOI":"10.1128\/jb.01069-08","type":"journal-article","created":{"date-parts":[[2008,10,18]],"date-time":"2008-10-18T00:59:46Z","timestamp":1224291586000},"page":"8197-8203","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":43,"title":["Phosphatidylethanolamine Synthesis Is Required for Optimal Virulence of\n            <i>Brucella abortus<\/i>"],"prefix":"10.1128","volume":"190","author":[{"given":"Lucas","family":"Bukata","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Biotecnolo\u0301gicas, Universidad Nacional de General San Marti\u0301n, CONICET, San Marti\u0301n 1650, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Altabe","sequence":"additional","affiliation":[{"name":"Instituto de Biologi\u0301a Molecular y Celular de Rosario and Departamento de Microbiologi\u0301a, Facultad de Ciencias Bioqui\u0301micas y Farmace\u0301uticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK, Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"de Mendoza","sequence":"additional","affiliation":[{"name":"Instituto de Biologi\u0301a Molecular y Celular de Rosario and Departamento de Microbiologi\u0301a, Facultad de Ciencias Bioqui\u0301micas y Farmace\u0301uticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK, Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo A.","family":"Ugalde","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnolo\u0301gicas, Universidad Nacional de General San Marti\u0301n, CONICET, San Marti\u0301n 1650, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego J.","family":"Comerci","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnolo\u0301gicas, Universidad Nacional de General San Marti\u0301n, CONICET, San Marti\u0301n 1650, Buenos Aires, Argentina"},{"name":"Comisio\u0301n Nacional de Energi\u0301a Ato\u0301mica, Divisio\u0301n Agropecuaria, Centro Ato\u0301mico Ezeiza 1804, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1038\/ni1202","volume":"6","year":"2005","unstructured":"Arellano-Reynoso, B., N. 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