{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:45:49Z","timestamp":1791117949144,"version":"4.1.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"24","license":[{"start":{"date-parts":[[2012,12,15]],"date-time":"2012-12-15T00:00:00Z","timestamp":1355529600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2012,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    LapF is a large secreted protein involved in microcolony formation and biofilm maturation in\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Pseudomonas putida<\/jats:named-content>\n                    . Its C-terminal domain shows the characteristics of proteins secreted through a type I secretion system and includes a predicted calcium binding motif. We provide experimental evidence of specific binding of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    to the purified C-terminal domain of LapF (CLapF). Calcium promotes the formation of large aggregates, which disappear in the presence of the calcium chelator EGTA. Immunolocalization of LapF also shows the tendency of this protein to accumulate\n                    <jats:italic>in vivo<\/jats:italic>\n                    in certain extracellular regions. These findings, along with results showing that calcium influences biofilm formation, lead us to propose a model in which\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. putida<\/jats:named-content>\n                    cells interact with each other via LapF in a calcium-dependent manner during the development of biofilms.\n                  <\/jats:p>","DOI":"10.1128\/jb.01094-12","type":"journal-article","created":{"date-parts":[[2012,10,6]],"date-time":"2012-10-06T01:55:38Z","timestamp":1349488538000},"page":"6782-6789","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Calcium Causes Multimerization of the Large Adhesin LapF and Modulates Biofilm Formation by Pseudomonas putida"],"prefix":"10.1128","volume":"194","author":[{"given":"Marta","family":"Mart\u00ednez-Gil","sequence":"first","affiliation":[{"name":"Department of Environmental Protection, Estaci\u00f3n Experimental del Zaid\u00edn, CSIC, Granada, Spain"},{"name":"Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Romero","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Kolter","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Espinosa-Urgel","sequence":"additional","affiliation":[{"name":"Department of Environmental Protection, Estaci\u00f3n Experimental del Zaid\u00edn, CSIC, Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.22.7490-7498.2004"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00642-12"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00727-07"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.06501-11"},{"key":"e_1_3_3_6_2","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1002\/j.1460-2075.1990.tb08117.x","article-title":"The Rhizobium nodulation gene nodO encodes a Ca2(+)-binding protein that is exported without N-terminal cleavage and is homologous to haemolysin and related proteins","volume":"9","author":"Economou A","year":"1990","unstructured":"EconomouA HamiltonWD JohnstonAW DownieJA. 1990. 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