{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T09:04:57Z","timestamp":1791104697791,"version":"4.1.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2009,2,15]],"date-time":"2009-02-15T00:00:00Z","timestamp":1234656000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2009,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Cyclic \u03b2-1,2-glucans (C\u03b2G) are periplasmic homopolysaccharides that have been shown to play an important role in several symbiotic and pathogenic relationships. Cyclic \u03b2-1,2-glucan synthase (Cgs), the enzyme responsible for the synthesis of C\u03b2G, is an integral membrane polyfunctional protein that catalyzes the four enzymatic activities (initiation, elongation, phosphorolysis, and cyclization) required for the synthesis of C\u03b2G. Recently, we have identified the glycosyltransferase and the \u03b2-1,2-glucooligosaccharide phosphorylase domains of\n            <jats:italic>Brucella abortus<\/jats:italic>\n            Cgs. In this study, we performed large-scale linker-scanning mutagenesis to gain further insight into the functional domains of Cgs. This analysis allowed us to construct a functional map of the enzyme and led to the identification of the minimal region required for the catalysis of initiation and elongation reactions. In addition, we identified the Cgs region (residues 991 to 1544) as being the protein domain required for cyclization and demonstrated that upon cyclization and releasing of the C\u03b2G, one or more glucose residues remain attached to the protein intermediate that serves as a primer for the next round of C\u03b2G synthesis. Finally, our results indicate that the overall control of the degree of polymerization of C\u03b2G is the result of a balance between elongation, phosphorolysis, and cyclization reactions.\n          <\/jats:p>","DOI":"10.1128\/jb.01108-08","type":"journal-article","created":{"date-parts":[[2008,12,13]],"date-time":"2008-12-13T02:48:38Z","timestamp":1229136518000},"page":"1230-1238","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Functional Mapping of\n            <i>Brucella abortus<\/i>\n            Cyclic \u03b2-1,2-Glucan Synthase: Identification of the Protein Domain Required for Cyclization"],"prefix":"10.1128","volume":"191","author":[{"given":"L. Soledad","family":"Guidolin","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Biotecnolo\u0301gicas, Universidad Nacional de San Marti\u0301n, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre\u0301s E.","family":"Ciocchini","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnolo\u0301gicas, Universidad Nacional de San Marti\u0301n, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nora","family":"In\u0303o\u0301n de Iannino","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnolo\u0301gicas, Universidad Nacional de San Marti\u0301n, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo A.","family":"Ugalde","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnolo\u0301gicas, Universidad Nacional de San Marti\u0301n, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.5.2563-2567.1990"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.17.5699-5707.2004"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1038\/ni1202","volume":"6","year":"2005","unstructured":"Arellano-Reynoso, B., N. 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