{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T18:44:38Z","timestamp":1790966678362,"version":"4.1.0"},"reference-count":55,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2007,2,15]],"date-time":"2007-02-15T00:00:00Z","timestamp":1171497600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2007,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Toxin-antitoxin loci belonging to the<jats:italic>yefM-yoeB<\/jats:italic>family are located in the chromosome or in some plasmids of several bacteria. We cloned the<jats:italic>yefM-yoeB<\/jats:italic>locus of<jats:italic>Streptococcus pneumoniae<\/jats:italic>, and these genes encode bona fide antitoxin (YefM<jats:sub><jats:italic>Spn<\/jats:italic><\/jats:sub>) and toxin (YoeB<jats:sub><jats:italic>Spn<\/jats:italic><\/jats:sub>) products. We showed that overproduction of YoeB<jats:sub><jats:italic>Spn<\/jats:italic><\/jats:sub>is toxic to<jats:italic>Escherichia coli<\/jats:italic>cells, leading to severe inhibition of cell growth and to a reduction in cell viability; this toxicity was more pronounced in an<jats:italic>E. coli<\/jats:italic>B strain than in two<jats:italic>E. coli<\/jats:italic>K-12 strains. The YoeB<jats:sub><jats:italic>Spn<\/jats:italic><\/jats:sub>-mediated toxicity could be reversed by the cognate antitoxin, YefM<jats:sub><jats:italic>Spn<\/jats:italic><\/jats:sub>, but not by overproduction of the<jats:italic>E. coli<\/jats:italic>YefM antitoxin. The pneumococcal proteins were purified and were shown to interact with each other both in vitro and in vivo. Far-UV circular dichroism analyses indicated that the pneumococcal antitoxin was partially, but not totally, unfolded and was different than its<jats:italic>E. coli<\/jats:italic>counterpart. Molecular modeling showed that the toxins belonging to the family were homologous, whereas the antitoxins appeared to be specifically designed for each bacterial locus; thus, the toxin-antitoxin interactions were adapted to the different bacterial environmental conditions. Both structural features, folding and the molecular modeled structure, could explain the lack of cross-complementation between the pneumococcal and<jats:italic>E. coli<\/jats:italic>antitoxins.<\/jats:p>","DOI":"10.1128\/jb.01130-06","type":"journal-article","created":{"date-parts":[[2007,2,3]],"date-time":"2007-02-03T02:42:29Z","timestamp":1170470549000},"page":"1266-1278","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":66,"title":["The<i>yefM-yoeB<\/i>Toxin-Antitoxin Systems of<i>Escherichia coli<\/i>and<i>Streptococcus pneumoniae<\/i>: Functional and Structural Correlation"],"prefix":"10.1128","volume":"189","author":[{"given":"Concha","family":"Nieto","sequence":"first","affiliation":[{"name":"Centro de Investigaciones Biolo\u0301gicas, CSIC, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izhack","family":"Cherny","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Tel Aviv 69978, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seok Kooi","family":"Khoo","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Malaysia University of Science and Technology, Petaling Jaya, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario Garci\u0301a","family":"de Lacoba","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Biolo\u0301gicas, CSIC, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wai Ting","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Malaysia University of Science and Technology, Petaling Jaya, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chew Chieng","family":"Yeo","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Malaysia University of Science and Technology, Petaling Jaya, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ehud","family":"Gazit","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Tel Aviv 69978, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Espinosa","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Biolo\u0301gicas, CSIC, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"6059","DOI":"10.1073\/pnas.93.12.6059","volume":"93","year":"1996","unstructured":"Aizenman, E., H. Engelberg-Kulka, and G. Glaser. 1996. An Escherichia coli chromosomal \u201caddiction module\u201d regulated by guanosine 3\u2032,5\u2032-bispyrophosphate: a model for programmed bacterial cell death. Proc. Natl. Acad. Sci. USA 93 : 6059-6063.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_3_2","first-page":"443","volume":"6","year":"1993","unstructured":"Andrade, M. A., P. Chac\u00f3n, J. J. Merolo, and F. Mor\u00e1n. 1993. Evaluation of secondary structure of proteins from UV circular dichroism spectra using an unsupervised learning neural network. Protein Eng. 6 : 443-454.","journal-title":"Protein Eng."},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1097\/00001432-199612000-00003","volume":"9","year":"1996","unstructured":"Baquero, F. 1996. Epidemiology and management of penicillin-resistant pneumococci. Curr. Opin. Infect. Dis. 9 : 372-379.","journal-title":"Curr. Opin. Infect. Dis."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1007\/BF00337764","volume":"210","year":"1987","unstructured":"Bravo, A., G. de Torrontegui, and R. D\u00edaz. 1987. Identification of components of a new stability system of plasmid R1, Par D, that is close to the origin of replication of this plasmid. Mol. Gen. Genet. 210 : 101-110.","journal-title":"Mol. Gen. Genet."},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"8252","DOI":"10.1074\/jbc.M308263200","volume":"279","year":"2004","unstructured":"Cherny, I., and E. Gazit. 2004. The YefM antitoxin defines a family of natively unfolded proteins: implications as a novel antibacterial target. J. Biol. Chem. 279 : 8252-8261.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"30063","DOI":"10.1074\/jbc.M506220200","volume":"280","year":"2005","unstructured":"Cherny, I., L. Rockah, and E. Gazit. 2005. The YoeB toxin is a folded protein that forms a physical complex with the unfolded YefM antitoxin. J. Biol. Chem. 280 : 30063-30072.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"1705","DOI":"10.1046\/j.1365-2958.2003.03941.x","volume":"51","year":"2004","unstructured":"Christensen, S. K., G. Maenhauf-Michel, N. Mine, S. Gothesman, K. Gerdes, and L. Van Melderen. 2004. Overproduction of the Lon protease triggers inhibition of translation in Escherichia coli: involvement of the yefM-yoeB toxin-antitoxin system. Mol. Microbiol. 51 : 1705-1717.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1111\/j.1365-2958.2004.04127.x","volume":"53","year":"2004","unstructured":"Christensen, S. K., and K. Gerdes. 2004. Delayed-relaxed response explained by hyperactivation of RelE. Mol. Microbiol. 53 : 587-597.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1046\/j.1365-2958.2003.03512.x","volume":"48","year":"2003","unstructured":"Christensen, S. K., and K. Gerdes. 2003. RelE toxins from bacteria and Archaea cleave mRNAs on translating ribosomes, which are rescued by tmRNA. Mol. Microbiol. 48 : 1389-1400.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"14328","DOI":"10.1073\/pnas.251327898","volume":"98","year":"2001","unstructured":"Christensen, S. K., M. Mikkelsen, K. Pedersen, and K. Gerdes. 2001. RelE, a global inhibitor of translation, is activated during nutritional stress. Proc. Natl. Acad. Sci. USA 98 : 14328-14333.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/S0022-2836(03)00922-7","volume":"332","year":"2003","unstructured":"Christensen, S. K., K. Pedersen, F. G. Hansen, and K. Gerdes. 2003. Toxin-antitoxin loci as stress-response-elements: ChpAK\/MazF and ChpBK cleave translated RNAs and are counteracted by tmRNA. J. Mol. Biol. 332 : 809-819.","journal-title":"J. Mol. Biol."},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1111\/j.1365-2958.2006.05270.x","volume":"61","year":"2006","unstructured":"Condon, C. 2006. Shutdown decay of mRNA. Mol. Microbiol. 61 : 573-583.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1073\/pnas.97.2.728","volume":"97","year":"2000","unstructured":"de la Hoz, A. B., S. Ayora, I. Sitkiewicz, S. Fernandez, R. Pankiewicz, J. C. Alonso, and P. Ceglowski. 2000. Plasmid copy-number control and better-than-random segregation genes of pSM19035 share a common regulator. Proc. Natl. Acad. Sci. USA 97 : 728-733.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_15_2","unstructured":"D\u00edaz-Orejas R. A. J. Mu\u00f1oz-G\u00f3mez and M. Lemonnier. 2003. The Kid-Kis toxin-antitoxin: a tale of selfishness survival and death. ELSO Gaz. Rev. http:\/\/www.the-elso-gazette.org\/magazines\/issue17\/reviews\/reviews1.asp ."},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1093\/embo-reports\/kvf152","volume":"3","year":"2002","unstructured":"Di Guilmi, A. M., and A. Dessen. 2002. New approaches towards the identification of antibiotic and vaccine targets in Streptococcus pneumoniae. EMBO Rep. 3 : 728-734.","journal-title":"EMBO Rep."},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1146\/annurev.micro.53.1.43","volume":"53","year":"1999","unstructured":"Engelberg-Kulka, H., and G. Glaser. 1999. Addiction modules and programmed cell death and antideath in bacterial cultures. Annu. Rev. Microbiol. 53 : 43-70.","journal-title":"Annu. Rev. Microbiol."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.tim.2003.12.008","volume":"12","year":"2004","unstructured":"Engelberg-Kulka, H., B. Sat, M. Reches, S. Amitai, and R. Hazan. 2004. Bacterial programmed cell death systems as targets for antibiotics. Trends Microbiol. 12 : 66-71.","journal-title":"Trends Microbiol."},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"2652","DOI":"10.1074\/jbc.274.5.2652","volume":"274","year":"1999","unstructured":"Gazit, E., and R. T. Sauer. 1999. Stability and DNA binding of the Phd protein of the phage P1 plasmid addiction system. J. Biol. Chem. 274 : 2652-2657.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_20_2","first-page":"49","year":"2000","unstructured":"Gerdes, K., S. Ayora, I. Canosa, P. Ceglowski, R. D\u00edaz-Orejas, T. Franch, A. P. Gultyaev, R. Bugge Jensen, I. Kobayashi, C. Macpherson, D. Summers, C. M. Thomas, and U. Zielenkiewicz. 2000. Bacterial plasmids and gene spread, p. 49-85. In C. M. Thomas (ed.), The horizontal gene pool. Harwood Academic Publishers, Amsterdam, The Netherlands.","journal-title":"The horizontal gene pool"},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1038\/nrmicro1147","volume":"3","year":"2005","unstructured":"Gerdes, K., K. S. Christensen, and A. Lobner-Olensen. 2005. Prokaryotic toxin-antitoxin stress response loci. Nat. Rev. Microbiol. 3 : 371-382.","journal-title":"Nat. Rev. Microbiol."},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1046\/j.1365-2958.1998.00993.x","volume":"29","year":"1998","unstructured":"Gotfredsen, M., and K. Gerdes. 1998. The Escherichia coli relBE genes belong to a new toxin-antitoxin gene family. Mol. Microbiol. 29 : 1065-1076.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1046\/j.1365-2958.2003.03387.x","volume":"47","year":"2003","unstructured":"Grady, R., and F. Hayes. 2003. Axe-Txe, a broad-spectrum proteic toxin-antitoxin system by a multidrug-resistant, clinical isolate of Enterococcus faecium. Mol. Microbiol. 47 : 1419-1432.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"2714","DOI":"10.1002\/elps.1150181505","volume":"18","year":"1997","unstructured":"Guex, N., and M. C. Peitsch. 1997. SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling. Electrophoresis 18 : 2714-2723.","journal-title":"Electrophoresis"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.19.5709-5717.2001"},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1002\/(SICI)1097-0134(19990515)35:3<307::AID-PROT4>3.0.CO;2-3","volume":"35","year":"1999","unstructured":"Johnson, W. C. 1999. Analyzing protein circular dichroism spectra for accurate secondary structures. Proteins 35 : 307-312.","journal-title":"Proteins"},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/j.molcel.2005.07.004","volume":"19","year":"2005","unstructured":"Kamada, K., and F. Hanaoka. 2005. Conformational change in the catalytic site of the ribonuclease YoeB toxin by YefM antitoxin. Mol. Cell 19 : 497-509.","journal-title":"Mol. Cell"},{"key":"e_1_3_2_28_2","unstructured":"Bioinformatics"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.4.628"},{"key":"e_1_3_2_30_2","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1093\/genetics\/60.4.685","volume":"60","year":"1968","unstructured":"Lacks, S. A. 1968. Genetic regulation of maltosaccharide utilization in Pneumococcus. Genetics 60 : 685-706.","journal-title":"Genetics"},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1006\/jmbi.1993.1521","volume":"233","year":"1993","unstructured":"Lehnherr, H., E. Maguin, S. Jafri, and M. B. Yarmolinsky. 1993. Plasmid addiction genes of bacteriophage P1: doc, which causes cell death on curing of prophage, and phd, which prevents host death when prophage is retained. J. Mol. Biol. 233 : 414-428.","journal-title":"J. Mol. Biol."},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"3918","DOI":"10.1093\/nar\/gkg463","volume":"31","year":"2003","unstructured":"Lemonnier, M., G. Ziegelin, T. Reick, A. Mu\u00f1oz-G\u00f3mez, R. D\u00edaz-Orejas, and E. Lanka. 2003. P1 Ban protein is a hexameric DNA helicase that interacts with and substitutes for Escherichia coli DnaB. Nucleic Acids Res. 31 : 3918-3928.","journal-title":"Nucleic Acids Res."},{"key":"e_1_3_2_33_2","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.femsle.2005.09.045","volume":"253","year":"2005","unstructured":"Lemos, J. A., T. A. Brown, Jr., J. Abranches, and R. A. Burne. 2005. Characteristics of Streptococcus mutans strains lacking the MazEF and RelBE toxin-antitoxin modules. FEMS Microbiol. Lett. 253 : 251-257.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_34_2","unstructured":"Maniatis T. E. F. Fritsch and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press Cold Spring Harbor NY."},{"key":"e_1_3_2_35_2","doi-asserted-by":"crossref","first-page":"37942","DOI":"10.1074\/jbc.M605198200","volume":"281","year":"2006","unstructured":"Mattison, K., J. S. Wilbur, M. So, and R. G. Brennan. 2006. Structure of FitAB from Neisseria gonorrhoeae bound to DNA reveals a tetramer of toxin-antitoxin heterodimers containing PIN domains and ribbon-helix-helix motifs. J. Biol. Chem. 281 : 37942-37951.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_36_2","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1021\/je60037a011","volume":"13","year":"1968","unstructured":"Mihalyi, E. J. 1968. Numerical values of the absorbances of the aromatic amino acids in acid, neutral, and alkaline solutions. Chem. Eng. Data 13 : 179-182.","journal-title":"Chem. Eng. Data"},{"key":"e_1_3_2_37_2","doi-asserted-by":"crossref","first-page":"1280","DOI":"10.1111\/j.1365-2958.2006.05027.x","volume":"59","year":"2006","unstructured":"Nieto, C., T. Pellicer, D. Balsa, S. K. Christensen, K. Gerdes, and M. Espinosa. 2006. The chromosomal relBE2 toxin-antitoxin locus of Streptococcus pneumoniae: characterization and use of a bioluminescence resonance energy transfer assay to detect toxin-antitoxin interaction. Mol. Microbiol. 59 : 1280-1296.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_38_2","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1093\/nar\/gki201","volume":"33","year":"2005","unstructured":"Pandey, D. P., and K. Gerdes. 2005. Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes. Nucleic Acids Res. 33 : 966-976.","journal-title":"Nucleic Acids Res."},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1046\/j.1365-2958.2002.03027.x","volume":"45","year":"2002","unstructured":"Pedersen, K., K. S. Christensen, and K. Gerdes. 2002. Rapid induction and reversal of a bacteriostatic conditions by controlled expression of toxins and antitoxins. Mol. Microbiol. 45 : 501-510.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_40_2","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/S0092-8674(02)01248-5","volume":"112","year":"2003","unstructured":"Pedersen, K., A. V. Zavialov, M. Y. Pavlov, J. Elf, K. Gerdes, and M. Ehrenberg. 2003. The bacterial toxin RelE displays codon-specific cleavage of mRNAs in the ribosomal A site. Cell 112 : 131-140.","journal-title":"Cell"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.10550"},{"key":"e_1_3_2_42_2","first-page":"33","volume":"6","year":"1981","unstructured":"Provencher, S. W., and J. Glockner. 1981. Estimation of globular protein secondary structure from circular dichroism. Biochemistry 6 : 33-37.","journal-title":"Biochemistry"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.6.2041-2045.2001"},{"key":"e_1_3_2_44_2","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1093\/protein\/11.9.739","volume":"11","year":"1998","unstructured":"Shindyalov, I. N., and P. E. Bourne. 1998. Protein structure alignment by incremental combinatorial extension (CE) of the optimal path. Protein Eng. 11 : 739-747.","journal-title":"Protein Eng."},{"key":"e_1_3_2_45_2","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/0926-6585(66)90333-5","volume":"112","year":"1966","unstructured":"Siegel, L. M., and K. J. Monty. 1966. Determination of molecular weights and frictional ratios of proteins in impure systems by the use of gel filtration and density gradient centrifugation. Applications to crude preparations of sulfite and hydroxylamine reductases. Biochim. Biophys. Acta 112 : 346-362.","journal-title":"Biochim. Biophys. Acta"},{"key":"e_1_3_2_46_2","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1110\/ps.8.2.370","volume":"8","year":"1999","unstructured":"Sreerama, N., S. Y. Venyaminov, and R. W. Woody. 1999. Estimation of the number of alpha-helical and beta-strand segments in proteins using circular dichroism spectroscopy. Protein Sci. 8 : 370-380.","journal-title":"Protein Sci."},{"key":"e_1_3_2_47_2","first-page":"252","volume":"15","year":"2000","unstructured":"Sreerama, N., and R. W. Woody. 2000. Estimation of protein secondary structure from circular dichroism spectra: comparison of CONTIN, SELCON, and CDSSTR methods with an expanded reference set. Anal. Biochem. 15 : 252-260.","journal-title":"Anal. Biochem."},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(90)85008-C"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1061217"},{"key":"e_1_3_2_50_2","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1006\/plas.2000.1506","volume":"45","year":"2001","unstructured":"Tian, Q.-B., M. Ohnishi, T. Murata, K. Nakayama, Y. Terawaki, and T. Hayashi. 2001. Specific protein-DNA and protein-protein interaction in the hig gene system, a plasmid-borne proteic killer gene system of plasmid Rts1. Plasmid 45 : 63-74.","journal-title":"Plasmid"},{"key":"e_1_3_2_51_2","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.mib.2004.08.007","volume":"7","year":"2004","unstructured":"Walsh, F. M., and S. G. B. Amyes. 2004. Microbiology and drug resistance mechanisms of fully resistant pathogens. Curr. Opin. Microbiol. 7 : 439-444.","journal-title":"Curr. Opin. Microbiol."},{"key":"e_1_3_2_52_2","first-page":"7","volume":"1","year":"2000","unstructured":"Whitney, C. G. 2000. Vaccination against pneumococcal disease: current questions and future opportunities. BioMed. Central 1 : 7.","journal-title":"BioMed. Central"},{"key":"e_1_3_2_53_2","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S1473-3099(01)00170-0","volume":"2","year":"2002","unstructured":"Williams, B. G., E. Gouws, C. Boschi-Pinto, J. Bryce, and C. Dye. 2002. Estimates of world-wide distribution of child deaths from acute respiratory infections. Lancet Infect. Dis. 2 : 25-32.","journal-title":"Lancet Infect. Dis."},{"key":"e_1_3_2_54_2","doi-asserted-by":"crossref","first-page":"5980","DOI":"10.1016\/S0021-9258(19)67694-5","volume":"266","year":"1991","unstructured":"Xiao, H., M. Kalman, K. Ikehara, S. Zemel, G. Glaser, and M. Cashel. 1991. Residual guanosine 3\u2032,5\u2032-bis-pyrophosphate synthetic activity of relA null mutants can be eliminated by spot null mutations. J. Biol. Chem. 266 : 5980-5990.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_55_2","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1006\/jmbi.1996.0230","volume":"258","year":"1996","unstructured":"Zhang, X., T. Reeder, and R. Schleif. 1996. Transcription activation parameters at ara p BAD . J. Mol. Biol. 258 : 14-24.","journal-title":"J. Mol. Biol."},{"key":"e_1_3_2_56_2","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1016\/S1097-2765(03)00402-7","volume":"12","year":"2003","unstructured":"Zhang, Y., J. Zhang, K. P. Hoeflich, M. Ikura, and M. Inouye. 2003. MazF cleaves cellular mRNAs specifically at ACA to block protein synthesis in Escherichia coli. Mol. Cell 12 : 913-923.","journal-title":"Mol. Cell"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.01130-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.01130-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,10]],"date-time":"2024-02-10T15:45:43Z","timestamp":1707579943000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.01130-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,2,15]]},"references-count":55,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,2,15]]}},"alternative-id":["10.1128\/JB.01130-06"],"URL":"https:\/\/doi.org\/10.1128\/jb.01130-06","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,2,15]]},"assertion":[{"value":"2006-07-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-10-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}