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Recent studies analyzed the carbon metabolism of<jats:italic>L. monocytogenes<\/jats:italic>by the<jats:sup>13<\/jats:sup>C isotopologue perturbation method in a defined minimal medium containing [U-<jats:sup>13<\/jats:sup>C<jats:sub>6<\/jats:sub>]glucose. It was shown that these bacteria produce oxaloacetate mainly by carboxylation of pyruvate due to an incomplete tricarboxylic acid cycle. Here, we report that a<jats:italic>pycA<\/jats:italic>insertion mutant defective in pyruvate carboxylase (PYC) still grows, albeit at a reduced rate, in brain heart infusion (BHI) medium but is unable to multiply in a defined minimal medium with glucose or glycerol as a carbon source. Aspartate and glutamate of the<jats:italic>pycA<\/jats:italic>mutant, in contrast to the wild-type strain, remain unlabeled when [U-<jats:sup>13<\/jats:sup>C<jats:sub>6<\/jats:sub>]glucose is added to BHI, indicating that the PYC-catalyzed carboxylation of pyruvate is the predominant reaction leading to oxaloacetate in<jats:italic>L. monocytogenes<\/jats:italic>. The<jats:italic>pycA<\/jats:italic>mutant is also unable to replicate in mammalian cells and exhibits high virulence attenuation in the mouse sepsis model.<\/jats:p>","DOI":"10.1128\/jb.01132-09","type":"journal-article","created":{"date-parts":[[2010,1,23]],"date-time":"2010-01-23T03:03:24Z","timestamp":1264215804000},"page":"1774-1784","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":71,"title":["Pyruvate Carboxylase Plays a Crucial Role in Carbon Metabolism of Extra- and Intracellularly Replicating<i>Listeria monocytogenes<\/i>"],"prefix":"10.1128","volume":"192","author":[{"given":"Jennifer","family":"Scha\u0308r","sequence":"first","affiliation":[{"name":"Lehrstuhl fu\u0308r Mikrobiologie, Biozentrum, Universita\u0308t Wu\u0308rzburg, D-97074 Wu\u0308rzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina","family":"Stoll","sequence":"additional","affiliation":[{"name":"Lehrstuhl fu\u0308r Mikrobiologie, Biozentrum, Universita\u0308t Wu\u0308rzburg, D-97074 Wu\u0308rzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Schauer","sequence":"additional","affiliation":[{"name":"Zentralinstitut fu\u0308r Erna\u0308hrungs- und Lebensmittelforschung, Abteilung Mikrobiologie, Technische Universita\u0308t Mu\u0308nchen, D-85350 Freising, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela I. M.","family":"Loeffler","sequence":"additional","affiliation":[{"name":"Lehrstuhl fu\u0308r Mikrobiologie, Biozentrum, Universita\u0308t Wu\u0308rzburg, D-97074 Wu\u0308rzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Eylert","sequence":"additional","affiliation":[{"name":"Lehrstuhl fu\u0308r Biochemie, Technische Universita\u0308t Mu\u0308nchen, D-85747 Garching, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biju","family":"Joseph","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Hygiene und Mikrobiologie, Universita\u0308t Wu\u0308rzburg, D-97080 Wu\u0308rzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Eisenreich","sequence":"additional","affiliation":[{"name":"Lehrstuhl fu\u0308r Biochemie, Technische Universita\u0308t Mu\u0308nchen, D-85747 Garching, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thilo M.","family":"Fuchs","sequence":"additional","affiliation":[{"name":"Zentralinstitut fu\u0308r Erna\u0308hrungs- und Lebensmittelforschung, Abteilung Mikrobiologie, Technische Universita\u0308t Mu\u0308nchen, D-85350 Freising, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner","family":"Goebel","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer-Institut fu\u0308r Hygiene und Medizinische Mikrobiologie, Ludwig-Maximilians-Universita\u0308t, D-80336 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1146\/annurev.micro.57.030502.090934","volume":"58","year":"2004","unstructured":"Dussurget, O., J. 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