{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:24:31Z","timestamp":1790691871108,"version":"4.1.0"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"24","license":[{"start":{"date-parts":[[2010,12,15]],"date-time":"2010-12-15T00:00:00Z","timestamp":1292371200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2010,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Methylosinus trichosporium<\/jats:italic>\n            OB3b (for \u201coddball\u201d strain 3b) is an obligate aerobic methane-oxidizing alphaproteobacterium that was originally isolated in 1970 by Roger Whittenbury and colleagues. This strain has since been used extensively to elucidate the structure and function of several key enzymes of methane oxidation, including both particulate and soluble methane monooxygenase (sMMO) and the extracellular copper chelator methanobactin. In particular, the catalytic properties of soluble methane monooxygenase from\n            <jats:italic>M. trichosporium<\/jats:italic>\n            OB3b have been well characterized in context with biodegradation of recalcitrant hydrocarbons, such as trichloroethylene. The sequence of the\n            <jats:italic>M. trichosporium<\/jats:italic>\n            OB3b genome is the first reported from a member of the\n            <jats:italic>Methylocystaceae<\/jats:italic>\n            family in the order\n            <jats:italic>Rhizobiales<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jb.01144-10","type":"journal-article","created":{"date-parts":[[2010,10,16]],"date-time":"2010-10-16T00:51:46Z","timestamp":1287190306000},"page":"6497-6498","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":104,"title":["Genome Sequence of the Obligate Methanotroph\n            <i>Methylosinus trichosporium<\/i>\n            Strain OB3b"],"prefix":"10.1128","volume":"192","author":[{"given":"Lisa Y.","family":"Stein","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukhwan","family":"Yoon","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, The University of Michigan, 1351 Beal Avenue, Ann Arbor, Michigan 48109-2125"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy D.","family":"Semrau","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, The University of Michigan, 1351 Beal Avenue, Ann Arbor, Michigan 48109-2125"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan A.","family":"DiSpirito","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Biophysics and Molecular Biology, University of Iowa, Ames, Iowa 50011"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Crombie","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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