{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T20:45:51Z","timestamp":1790541951720,"version":"4.1.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The ability to capture iron is a challenge for most bacteria. The neonatal meningitis\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>\n            strain S88 possesses several iron uptake systems, notably including siderophores. Transcriptional analysis of the ColV plasmid pS88 has shown strong induction of a previously undescribed gene with low identity to three\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. coli<\/jats:named-content>\n            chromosomal genes encoding phospho-2-dehydro-3-deoxyheptonate aldolases involved in aromatic amino acid and catecholate\/phenolate siderophore biosynthesis through the shikimate pathway. Here, we investigated the role of this gene,\n            <jats:italic>\n              ssbL\n              <jats:sub>p<\/jats:sub>\n            <\/jats:italic>\n            (\n            <jats:italic>ssbL<\/jats:italic>\n            carried on the plasmid), in siderophore biosynthesis and, consequently, in S88 virulence. We constructed an S88 mutant designated S88 \u0394\n            <jats:italic>\n              ssbL\n              <jats:sub>p<\/jats:sub>\n            <\/jats:italic>\n            , which exhibited reduced growth under low-iron conditions compared to the wild-type strain. Liquid chromatography-mass spectroscopy analysis of culture supernatants showed that the mutant secreted significantly smaller amounts of enterobactin, salmochelin SX, and yersiniabactin than the wild-type strain. The mutant was also less virulent in a neonatal rat sepsis model, with significantly lower bacteremia and mortality. Supplementation with chorismate, the final product of the shikimate pathway, restored the wild-type phenotype\n            <jats:italic>in vitro<\/jats:italic>\n            . In a collection of human extraintestinal\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. coli<\/jats:named-content>\n            isolates, we found that\n            <jats:italic>ssbL<\/jats:italic>\n            was present only in strains harboring the\n            <jats:italic>iro<\/jats:italic>\n            locus, encoding salmochelins, and was located either on the chromosome or on plasmids. Acquisition of the\n            <jats:italic>iro<\/jats:italic>\n            locus has been accompanied by acquisition of the auxiliary gene\n            <jats:italic>ssbL<\/jats:italic>\n            , which boosts the metabolic pathway essential for catecholate\/phenolate siderophore biosynthesis and could represent potential therapeutic targets.\n          <\/jats:p>","DOI":"10.1128\/jb.01153-13","type":"journal-article","created":{"date-parts":[[2014,1,18]],"date-time":"2014-01-18T02:01:14Z","timestamp":1390010474000},"page":"1343-1349","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["The\n            <i>ssbL<\/i>\n            Gene Harbored by the ColV Plasmid of an Escherichia coli Neonatal Meningitis Strain Is an Auxiliary Virulence Factor Boosting the Production of Siderophores through the Shikimate Pathway"],"prefix":"10.1128","volume":"196","author":[{"given":"Chlo\u00e9","family":"Lema\u00eetre","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris Diderot, Sorbonne Paris Cit\u00e9, EA3105, Paris, France"},{"name":"Service de Microbiologie, H\u00f4pital Robert-Debr\u00e9, AP-HP, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Bidet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Diderot, Sorbonne Paris Cit\u00e9, EA3105, Paris, France"},{"name":"Service de Microbiologie, H\u00f4pital Robert-Debr\u00e9, AP-HP, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Fran\u00e7ois","family":"Benoist","sequence":"additional","affiliation":[{"name":"Service de Biochimie-Hormonologie, H\u00f4pital Robert Debr\u00e9, AP-HP, Paris, France"},{"name":"Universit\u00e9 Paris-Sud, Chatenay-Malabry, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitri","family":"Schlemmer","sequence":"additional","affiliation":[{"name":"Service de Biochimie-Hormonologie, H\u00f4pital Robert Debr\u00e9, AP-HP, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elsa","family":"Sobral","sequence":"additional","affiliation":[{"name":"Service de Microbiologie, H\u00f4pital Robert-Debr\u00e9, AP-HP, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"d'Humi\u00e8res","sequence":"additional","affiliation":[{"name":"Service de Microbiologie, H\u00f4pital Robert-Debr\u00e9, AP-HP, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Bonacorsi","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Diderot, Sorbonne Paris Cit\u00e9, EA3105, Paris, France"},{"name":"Service de Microbiologie, H\u00f4pital Robert-Debr\u00e9, AP-HP, Paris, 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