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Double-immunofluorescence analysis suggested that DivIVA is recruited to the septum at a later stage than FtsZ and is retained at the poles after cell separation. All the other cell division proteins that we tested were localized in the<jats:italic>divIVA<\/jats:italic>null mutant, although the percentage of cells having constricted Z rings was significantly reduced. In agreement with its localization profile and consistent with its coiled-coil nature, DivIVA interacted with itself and with a number of known or putative<jats:italic>S. pneumoniae<\/jats:italic>cell division proteins. Finally, a missense<jats:italic>divIVA<\/jats:italic>mutant, obtained by allelic replacement, allowed us to correlate, at the molecular level, the specific interactions and some of the facets of the<jats:italic>divIVA<\/jats:italic>mutant phenotype. Taken together, the results suggest that although the possibility of a direct role in chromosome segregation cannot be ruled out, DivIVA in<jats:italic>S. pneumoniae<\/jats:italic>seems to be primarily involved in the formation and maturation of the cell poles. The localization and the interaction properties of DivIVA<jats:sub>SPN<\/jats:sub>raise the intriguing possibility that a common, MinCD-independent function evolved differently in the various host backgrounds.<\/jats:p>","DOI":"10.1128\/jb.01168-06","type":"journal-article","created":{"date-parts":[[2006,11,14]],"date-time":"2006-11-14T01:39:08Z","timestamp":1163468348000},"page":"1288-1298","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":125,"title":["<i>Streptococcus pneumoniae<\/i>DivIVA: Localization and Interactions in a MinCD-Free Context"],"prefix":"10.1128","volume":"189","author":[{"given":"Daniela","family":"Fadda","sequence":"first","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biomediche, Sez. 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