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The Lyme disease spirochete\n                    <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                    has two plasmid-borne putative R-M genes,\n                    <jats:italic>bbe02<\/jats:italic>\n                    and\n                    <jats:italic>bbq67<\/jats:italic>\n                    , whose presence limits transformation by shuttle vector DNA from\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    . We show that both the\n                    <jats:italic>bbe02<\/jats:italic>\n                    and\n                    <jats:italic>bbq67<\/jats:italic>\n                    loci in recipient\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    limit transformation with shuttle vector DNA from\n                    <jats:italic>E. coli<\/jats:italic>\n                    , irrespective of its\n                    <jats:italic>dam<\/jats:italic>\n                    ,\n                    <jats:italic>dcm<\/jats:italic>\n                    , or\n                    <jats:italic>hsd<\/jats:italic>\n                    methylation status. However, plasmid DNA purified from\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    transformed nai\u0308ve\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    much more efficiently than plasmid DNA from\n                    <jats:italic>E. coli<\/jats:italic>\n                    , particularly when the\n                    <jats:italic>bbe02<\/jats:italic>\n                    and\n                    <jats:italic>bbq67<\/jats:italic>\n                    genotypes of the\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    DNA source matched those of the recipient. We detected adenine methylation of plasmid DNA prepared from\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    that carried\n                    <jats:italic>bbe02<\/jats:italic>\n                    and\n                    <jats:italic>bbq67<\/jats:italic>\n                    . These results indicate that the\n                    <jats:italic>bbe02<\/jats:italic>\n                    and\n                    <jats:italic>bbq67<\/jats:italic>\n                    loci of\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    encode distinct R-M enzymes that methylate endogenous DNA and cleave foreign DNA lacking the same sequence-specific modification. Our findings have basic implications for horizontal gene transfer among\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    strains with distinct plasmid contents. Further characterization and identification of the nucleotide sequences recognized by BBE02 and BBQ67 will facilitate efficient genetic manipulation of this pathogenic spirochete.\n                  <\/jats:p>","DOI":"10.1128\/jb.01176-10","type":"journal-article","created":{"date-parts":[[2010,12,30]],"date-time":"2010-12-30T22:43:31Z","timestamp":1293749011000},"page":"1161-1171","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":97,"title":["Defining the Plasmid-Borne Restriction-Modification Systems of the Lyme Disease Spirochete\n                    <i>Borrelia burgdorferi<\/i>"],"prefix":"10.1128","volume":"193","author":[{"given":"Ryan O. 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