{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:06:37Z","timestamp":1790787997662,"version":"4.1.0"},"reference-count":62,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2010,3,15]],"date-time":"2010-03-15T00:00:00Z","timestamp":1268611200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2010,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Catechols are central intermediates in the metabolism of aromatic compounds. Degradation of 4-methylcatechol via intradiol cleavage usually leads to the formation of 4-methylmuconolactone (4-ML) as a dead-end metabolite. Only a few microorganisms are known to mineralize 4-ML. The<jats:italic>mml<\/jats:italic>gene cluster of<jats:italic>P<\/jats:italic><jats:italic>seudomonas<\/jats:italic><jats:italic>reinekei<\/jats:italic>MT1, which encodes enzymes involved in the metabolism of 4-ML, is shown here to encode 10 genes found in a 9.4-kb chromosomal region. Reverse transcription assays revealed that these genes form a single operon, where their expression is controlled by two promoters. Promoter fusion assays identified 4-methyl-3-oxoadipate as an inducer. Mineralization of 4-ML is initiated by the 4-methylmuconolactone methylisomerase encoded by<jats:italic>mmlI<\/jats:italic>. This reaction produces 3-ML and is followed by a rearrangement of the double bond catalyzed by the methylmuconolactone isomerase encoded by<jats:italic>mmlJ<\/jats:italic>. Deletion of<jats:italic>mmlL<\/jats:italic>, encoding a protein of the metallo-\u03b2-lactamase superfamily, resulted in a loss of the capability of the strain MT1 to open the lactone ring, suggesting its function as a 4-methyl-3-oxoadipate enol-lactone hydrolase. Further metabolism can be assumed to occur by analogy with reactions known from the 3-oxoadipate pathway.<jats:italic>mmlF<\/jats:italic>and<jats:italic>mmlG<\/jats:italic>probably encode a 4-methyl-3-oxoadipyl-coenzyme A (CoA) transferase, and the<jats:italic>mmlC<\/jats:italic>gene product functions as a thiolase, transforming 4-methyl-3-oxoadipyl-CoA into methylsuccinyl-CoA and acetyl-CoA, as indicated by the accumulation of 4-methyl-3-oxoadipate in the respective deletion mutant. Accumulation of methylsuccinate by an<jats:italic>mmlK<\/jats:italic>deletion mutant indicates that the encoded acetyl-CoA hydrolase\/transferase is crucial for channeling methylsuccinate into the central metabolism.<\/jats:p>","DOI":"10.1128\/jb.01208-09","type":"journal-article","created":{"date-parts":[[2010,1,9]],"date-time":"2010-01-09T02:57:25Z","timestamp":1263005845000},"page":"1543-1552","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Modified 3-Oxoadipate Pathway for the Biodegradation of Methylaromatics in<i>Pseudomonas reinekei<\/i>MT1"],"prefix":"10.1128","volume":"192","author":[{"given":"Macarena","family":"Mari\u0301n","sequence":"first","affiliation":[{"name":"Department of Microbial Pathogenesis, Helmholtz Zentrum fu\u0308r Infektionsforschung (HZI), Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danilo","family":"Pe\u0301rez-Pantoja","sequence":"additional","affiliation":[{"name":"Departamento de Gene\u0301tica Molecular y Microbiologi\u0301a, Facultad de Ciencias Biolo\u0301gicas, NM-EMBA, CASEB, P. Universidad Cato\u0301lica de Chile, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raul","family":"Donoso","sequence":"additional","affiliation":[{"name":"Departamento de Gene\u0301tica Molecular y Microbiologi\u0301a, Facultad de Ciencias Biolo\u0301gicas, NM-EMBA, CASEB, P. Universidad Cato\u0301lica de Chile, Santiago, Chile"},{"name":"Facultad de Ingenieri\u0301a y Ciencias, Universidad Adolfo Iba\u0301n\u0303ez, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor","family":"Wray","sequence":"additional","affiliation":[{"name":"Division of Structural Biology, Helmholtz Zentrum fu\u0308r Infektionsforschung (HZI), Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernardo","family":"Gonza\u0301lez","sequence":"additional","affiliation":[{"name":"Departamento de Gene\u0301tica Molecular y Microbiologi\u0301a, Facultad de Ciencias Biolo\u0301gicas, NM-EMBA, CASEB, P. Universidad Cato\u0301lica de Chile, Santiago, Chile"},{"name":"Facultad de Ingenieri\u0301a y Ciencias, Universidad Adolfo Iba\u0301n\u0303ez, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dietmar H.","family":"Pieper","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis, Helmholtz Zentrum fu\u0308r Infektionsforschung (HZI), Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1111\/j.1365-2958.2006.05238.x","volume":"61","year":"2006","unstructured":"Alber, B. E., R. R. Spanheimer, C. Ebenau-Jehle, and G. Fuchs. 2006. Study of an alternate glyoxylate cycle for acetate assimilation by Rhodobacter sphaeroides. Mol. Microbiol. 61 : 297-309.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1111\/j.1432-1033.1992.tb16835.x","volume":"205","year":"1992","unstructured":"Altenschmidt, U., and G. Fuchs. 1992. Novel aerobic 2-aminobenzoate metabolism. Purification and characterization of 2-aminobenzoate-CoA ligase, localisation of the genes on a 8-kbp plasmid, and cloning and sequencing of the genes from a denitrifying Pseudomonas sp. Eur. J. Biochem. 205 : 721-727.","journal-title":"Eur. J. Biochem."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.24.8450-8461.2005"},{"key":"e_1_3_2_6_2","first-page":"233","volume":"50","year":"1988","unstructured":"Bruce, N. C., and R. B. Cain. 1988. \u03b2-Methylmuconolactone, a key intermediate in the dissimilation of methylaromatic compounds by a modified 3-oxoadipate pathway evolved in nocardioform actinomycetes. FEMS Microbiol. Lett. 50 : 233-239.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1042\/bj2620303","volume":"262","year":"1989","unstructured":"Bruce, N. C., R. B. Cain, D. H. Pieper, and K.-H. Engesser. 1989. Purification and characterization of 4-methylmuconolactone methyl-isomerase, a novel enzyme of the modified 3-oxoadipate pathway in nocardioform actionomycetes. Biochem. J. 262 : 303-312.","journal-title":"Biochem. J."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.1.396-399.2005"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01192-06"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1099\/ijs.0.64703-0","volume":"57","year":"2007","unstructured":"Camara, B., C. Strompl, S. Verbarg, C. Sproer, D. H. Pieper, and B. J. Tindall. 2007. Pseudomonas reinekei sp. nov., Pseudomonas moorei sp. nov. and Pseudomonas mohnii sp. nov., novel species capable of degrading chlorosalicylates or isopimaric acid. Int. J. Syst. Evol. Microbiol. 57 : 923-931.","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00021-08"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1042\/bj1210089","volume":"121","year":"1971","unstructured":"Catelani, D., A. Fiecchi, and E. Galli. 1971. (+)-\u03b3-Carboxymethyl-\u03b3-methyl-\u0394\u03b1-butenolide. A 1,2-ring-fission product of 4-methylcatechol by Pseudomonas desmolyticum. Biochem. J. 121 : 89-92.","journal-title":"Biochem. J."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.24.6668-6673.1998"},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"5520","DOI":"10.1021\/ja00252a035","volume":"109","year":"1987","unstructured":"Chari, R., C. Whitman, J. Kozarich, K. Ngai, and L. Ornston. 1987. Absolute stereochmical course of muconolactone \u0394-isomerase and of 4-carboxymuconolactone decarboxylase: a 1H NMR \u201cricochet\u201d analysis. J. Am. Chem. Soc. 109 : 5520-5521.","journal-title":"J. Am. Chem. Soc."},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1099\/mic.0.28412-0","volume":"152","year":"2006","unstructured":"Charrier, C., G. J. Duncan, M. D. Reid, G. J. Rucklidge, D. Henderson, O. Young, V. J. Russell, R. I. Aminov, H. J. Flint, and P. Louis. 2006. A novel class of CoA-transferase involved in short-chain fatty acid metabolism in butyrate-producing human colonic bacteria. Microbiology 152 : 179-185.","journal-title":"Microbiology"},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"1582","DOI":"10.1126\/science.3538420","volume":"234","year":"1986","unstructured":"Chu, G., D. Vollrath, and R. W. Davis. 1986. Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science 234 : 1582-1585.","journal-title":"Science"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.pep.2006.03.013","volume":"49","year":"2006","unstructured":"Chu, X. Y., N. F. Wu, M. J. Deng, J. Tian, B. Yao, and Y. L. Fan. 2006. Expression of organophosphorus hydrolase OPHC2 in Pichia pastoris: purification and characterization. Protein Expr. Purif. 49 : 9-14.","journal-title":"Protein Expr. Purif."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1007\/s00253-009-1919-6","volume":"83","year":"2009","unstructured":"Chung, A., Q. Liu, S. P. Ouyang, Q. Wu, and G. Q. Chen. 2009. Microbial production of 3-hydroxydodecanoic acid by pha operon and fadBA knockout mutant of Pseudomonas putida KT2442 harboring tesB gene. Appl. Microbiol. Biotechnol. 83 : 513-519.","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/S0022-2836(67)80070-6","volume":"23","year":"1967","unstructured":"Clark, J., and O. Maaloe. 1967. DNA replication and the division cycle in Escherichia coli. J. Mol. Biol. 23 : 99-112.","journal-title":"J. Mol. Biol."},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"3305","DOI":"10.1111\/j.1462-2920.2008.01722.x","volume":"10","year":"2008","unstructured":"de las Heras, A., C. A. Carreno, and V. de Lorenzo. 2008. Stable implantation of orthogonal sensor circuits in Gram-negative bacteria for environmental release. Environ. Microbiol. 10 : 3305-3316.","journal-title":"Environ. Microbiol."},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(94)35157-0"},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/s00203-007-0226-9","volume":"188","year":"2007","unstructured":"Di Gennaro, P., S. Ferrara, I. Ronco, E. Galli, G. Sello, M. Papacchini, and G. Bestetti. 2007. Styrene lower catabolic pathway in Pseudomonas fluorescens ST: identification and characterization of genes for phenylacetic acid degradation. Arch. Microbiol. 188 : 117-125.","journal-title":"Arch. Microbiol."},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/BF00696222","volume":"99","year":"1974","unstructured":"Dorn, E., M. Hellwig, W. Reineke, and H.-J. Knackmuss. 1974. Isolation and characterization of a 3-chlorobenzoate degrading pseudomonad. Arch. Microbiol. 99 : 61-70.","journal-title":"Arch. Microbiol."},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1186\/1471-2105-5-113","volume":"5","year":"2004","unstructured":"Edgar, R. C. 2004. MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformics 5 : 113.","journal-title":"BMC Bioinformics"},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0378-1119(97)00565-9","volume":"206","year":"1998","unstructured":"Erb, R. W., K. N. Timmis, and D. H. Pieper. 1998. Characterization of a gene cluster from Ralstonia eutropha JMP134 encoding metabolism of 4-methylmuconolactone. Gene 206 : 53-62.","journal-title":"Gene"},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"32283","DOI":"10.1074\/jbc.M805527200","volume":"283","year":"2008","unstructured":"Erb, T. J., J. Retey, G. Fuchs, and B. E. Alber. 2008. Ethylmalonyl-CoA mutase from Rhodobacter sphaeroides defines a new subclade of coenzyme B12-dependent acyl-CoA mutases. J. Biol. Chem. 283 : 32283-32293.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.5.1072-1081.1998"},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1196\/annals.1419.010","volume":"1125","year":"2008","unstructured":"Fuchs, G. 2008. Anaerobic metabolism of aromatic compounds. Ann. N. Y. Acad. Sci. 1125 : 82-99.","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.2.956-966.1990"},{"key":"e_1_3_2_30_2","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1146\/annurev.micro.50.1.553","volume":"50","year":"1996","unstructured":"Harwood, C. S., and R. E. Parales. 1996. The beta-ketoadipate pathway and the biology of self-identity. Annu. Rev. Microbiol. 50 : 553-590.","journal-title":"Annu. Rev. Microbiol."},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","first-page":"6557","DOI":"10.1128\/JB.172.11.6557-6567.1990","volume":"172","year":"1990","unstructured":"Herrero, M., V. de Lorenzo, and K. N. Timmis. 1990. Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria. J. Bacteriol. 172 : 6557-6567.","journal-title":"J. Bacteriol."},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0378-1119(98)00130-9","volume":"212","year":"1998","unstructured":"Hoang, T. T., R. R. Karkhoff-Schweizer, A. J. Kutchma, and H. P. Schweizer. 1998. A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 212 : 77-86.","journal-title":"Gene"},{"key":"e_1_3_2_33_2","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1046\/j.1462-2920.2003.00541.x","volume":"6","year":"2004","unstructured":"Junca, H., and D. H. Pieper. 2004. Functional gene diversity analysis in BTEX contaminated soils by means of PCR-SSCP DNA fingerprinting: comparative diversity assessment against bacterial isolates and PCR-DNA clone libraries. Environ. Microbiol. 6 : 95-110.","journal-title":"Environ. Microbiol."},{"key":"e_1_3_2_34_2","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/S1360-1385(02)02251-3","volume":"7","year":"2002","unstructured":"Karimi, M., D. Inze, and A. Depicker. 2002. GATEWAY vectors for Agrobacterium-mediated plant transformation. Trends Plant Sci. 7 : 193-195.","journal-title":"Trends Plant Sci."},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.1.207-215.2002"},{"key":"e_1_3_2_36_2","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1007\/BF01278610","volume":"2","year":"1976","unstructured":"Knackmuss, H.-J., M. Hellwig, H. Lackner, and W. Otting. 1976. Cometabolism of 3-methylbenzoate and methylcatechols by a 3-chlorobenzoate utilizing Pseudomonas: accumulation of (+)-2,5-dihydro-4-methyl- and (+)-2,5-dihydro-2-methyl-5-oxo-furan-2-acetic acid. Eur. J. Appl. Microbiol. 2 : 267-276.","journal-title":"Eur. J. Appl. Microbiol."},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.4.1523-1526.2005"},{"key":"e_1_3_2_38_2","doi-asserted-by":"crossref","first-page":"3431","DOI":"10.1099\/00221287-148-11-3431","volume":"148","year":"2002","unstructured":"Ledger, T., D. H. Pieper, D. Perez-Pantoja, and B. Gonzalez. 2002. Novel insights into the interplay between peripheral reactions encoded by xyl genes and the chlorocatechol pathway encoded by tfd genes for the degradation of chlorobenzoates by Ralstonia eutropha JMP134. Microbiology 148 : 3431-3440.","journal-title":"Microbiology"},{"key":"e_1_3_2_39_2","unstructured":"Experiments in molecular genetics 1972"},{"key":"e_1_3_2_40_2","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1111\/j.1432-1033.1972.tb01914.x","volume":"28","year":"1972","unstructured":"Murray, K., C. J. Duggleby, J. M. S. Trepat, and P. A. Williams. 1972. The metabolism of benzoate and methylbenzoates via the meta-cleavage by Pseudomonas arvilla mt-2. Eur. J. Biochem. 28 : 301-310.","journal-title":"Eur. J. Biochem."},{"key":"e_1_3_2_41_2","doi-asserted-by":"crossref","first-page":"11044","DOI":"10.1016\/S0021-9258(18)99125-8","volume":"266","year":"1991","unstructured":"Naggert, J., M. L. Narasimhan, L. DeVeaux, H. Cho, Z. I. Randhawa, J. E. J. Cronan, B. N. Green, and S. Smith. 1991. Cloning, sequencing, and characterization of Escherichia coli thioesterase II. J. Biol. Chem. 266 : 11044-11050.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.23.6790-6800.2003"},{"key":"e_1_3_2_43_2","doi-asserted-by":"crossref","first-page":"6419","DOI":"10.1073\/pnas.95.11.6419","volume":"95","year":"1998","unstructured":"Olivera, E. R., B. Minambres, B. Garcia, C. Muniz, M. A. Moreno, A. Ferrandez, E. Diaz, J. L. Garcia, and J. M. Luengo. 1998. Molecular characterization of the phenylacetic acid catabolic pathway in Pseudomonas putida U: the phenylacetyl-CoA catabolon. Proc. Natl. Acad. Sci. U. S. A. 95 : 6419-6424.","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"e_1_3_2_44_2","doi-asserted-by":"crossref","first-page":"3776","DOI":"10.1016\/S0021-9258(18)99839-X","volume":"241","year":"1966","unstructured":"Ornston, L., and R. Stamier. 1966. The conversion of catechol and protocatechuate to beta-ketoadipate by Pseudomonas putida. 1. Biochemistry. J. Biol. Chem. 241 : 3776-3786.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.14.4657-4666.1992"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.12.4050-4063.2005"},{"key":"e_1_3_2_47_2","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1111\/j.1574-6976.2008.00122.x","volume":"32","year":"2008","unstructured":"Perez-Pantoja, D., R. De la Iglesia, D. H. Pieper, and B. Gonzalez. 2008. Metabolic reconstruction of aromatic compounds degradation from the genome of the amazing pollutant-degrading bacterium Cupriavidus necator JMP134. FEMS Microbiol. Rev. 32 : 736-794.","journal-title":"FEMS Microbiol. Rev."},{"key":"e_1_3_2_48_2","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/j.1574-6968.1985.tb00836.x","volume":"29","year":"1985","unstructured":"Pieper, D. H., K.-H. Engesser, R. H. Don, K. N. Timmis, and H.-J. Knackmuss. 1985. Modified ortho-cleavage pathway in Alcaligenes eutrophus JMP134 for the degradation of 4-methylcatechol. FEMS Microbiol. Lett. 29 : 63-67.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_49_2","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1007\/BF00406566","volume":"151","year":"1989","unstructured":"Pieper, D. H., K.-H. Engesser, and H.-J. Knackmuss. 1989. Regulation of catabolic pathways of phenoxyacetic acids and phenols in Alcaligenes eutrophus JMP 134. Arch. Microbiol. 151 : 365-371.","journal-title":"Arch. Microbiol."},{"key":"e_1_3_2_50_2","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1007\/BF00249121","volume":"160","year":"1993","unstructured":"Pieper, D. H., K. Stadler-Fritsche, K. H. Engesser, and H. J. Knackmuss. 1993. Metabolism of 2-chloro-4-methylphenoxyacetate by AIcaligenes eutrophus JMP 134. Arch. Microbiol. 160 : 169-178.","journal-title":"Arch. Microbiol."},{"key":"e_1_3_2_51_2","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1042\/bj2710529","volume":"271","year":"1990","unstructured":"Pieper, D. H., K. Stadler-Fritzsche, H.-J. Knackmuss, K. H. Engesser, N. C. Bruce, and R. B. Cain. 1990. Purification and characterization of 4-methylmuconolactone methylisomerase, a novel enzyme of the modfied 3-oxoadipate pathway in the Gram-negative bacterium Alcaligenes eutrophus JMP 134. Biochem. J. 271 : 529-534.","journal-title":"Biochem. J."},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.163.3.1126-1135.1985"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.163.3.1136-1141.1985"},{"key":"e_1_3_2_54_2","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/s002030050466","volume":"168","year":"1997","unstructured":"Prucha, M., A. Peterseim, and D. H. Pieper. 1997. Evidence for an isomeric muconolactone isomerase involved in the metabolism of 4-methylmuconolactone by Alcaligenes eutrophus JMP134. Arch. Microbiol. 168 : 33-38.","journal-title":"Arch. Microbiol."},{"key":"e_1_3_2_55_2","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1111\/j.1432-1033.1996.00350.x","volume":"237","year":"1996","unstructured":"Prucha, M., A. Peterseim, K. N. Timmis, and D. H. Pieper. 1996. Muconolactone isomerase of the 3-oxoadipate pathway catalyzes dechlorination of 5-chloro-substituted muconolactones. Eur. J. Biochem. 237 : 350-356.","journal-title":"Eur. J. Biochem."},{"key":"e_1_3_2_56_2","first-page":"406","volume":"4","year":"1987","unstructured":"Saitou, N., and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4 : 406-425.","journal-title":"Mol. Biol. Evol."},{"key":"e_1_3_2_57_2","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1111\/j.1432-1033.1972.tb01920.x","volume":"28","year":"1972","unstructured":"Sala-Trepat, J. M., K. Murray, and P. A. Williams. 1972. The metabolic divergence in the meta-cleavage of catechols by Pseudomonas putida NCIB 10015. Eur. J. Biochem. 28 : 347-356.","journal-title":"Eur. J. Biochem."},{"key":"e_1_3_2_58_2","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1042\/bj1920339","volume":"192","year":"1980","unstructured":"Schmidt, E., and H.-J. Knackmuss. 1980. Chemical structure and biodegradability of halogenated aromatic compounds. Conversion of chlorinated muconic acids into maleoylacetic acid. Biochem. J. 192 : 339-347.","journal-title":"Biochem. J."},{"key":"e_1_3_2_59_2","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.2144\/01316bm06","volume":"31","year":"2001","unstructured":"Schweizer, H. P., and R. Chuanchuen. 2001. Small broad-host-range lacZ operon fusion vector with low background activity. Biotechniques 31 : 1258-1262.","journal-title":"Biotechniques"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msm092"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.9.3290-3299.1998"},{"key":"e_1_3_2_62_2","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/S0958-1669(00)00205-6","volume":"12","year":"2001","unstructured":"Watanabe, K. 2001. Microorganisms relevant to bioremediation. Curr. Opin. Biotechnol. 12 : 237-241.","journal-title":"Curr. Opin. Biotechnol."},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.18.5935-5942.1997"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.01208-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.01208-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T16:46:06Z","timestamp":1711125966000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.01208-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3,15]]},"references-count":62,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2010,3,15]]}},"alternative-id":["10.1128\/JB.01208-09"],"URL":"https:\/\/doi.org\/10.1128\/jb.01208-09","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3,15]]},"assertion":[{"value":"2009-09-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-12-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}