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Biochemical analyses confirmed that BA-Stp1 is a PP2C phosphatase and dephosphorylates phosphoserine and phosphothreonine residues. Treatment of BA-Stk1 with BA-Stp1 altered BA-Stk1 kinase activity, indicating that the enzymatic function of BA-Stk1 can be influenced by BA-Stp1 dephosphorylation. Using a combination of mass spectrometry and mutagenesis approaches, three phosphorylated residues, T165, S173, and S214, in BA-Stk1 were identified as putative regulatory targets of BA-Stp1. Further analysis found that T165 and S173 were necessary for optimal substrate phosphorylation, while S214 was necessary for complete ATP hydrolysis, autophosphorylation, and substrate phosphorylation. These findings provide insight into a previously undescribed Stp\/Stk pair in\n            <jats:italic>B. anthracis<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jb.01221-09","type":"journal-article","created":{"date-parts":[[2009,11,14]],"date-time":"2009-11-14T02:10:03Z","timestamp":1258164603000},"page":"400-409","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Regulatory Interactions of a Virulence-Associated Serine\/Threonine Phosphatase-Kinase Pair in\n            <i>Bacillus anthracis<\/i>"],"prefix":"10.1128","volume":"192","author":[{"given":"Salika M.","family":"Shakir","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie M.","family":"Bryant","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason L.","family":"Larabee","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine E.","family":"Hamm","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Lovchik","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of New Mexico Health Sciences Center, Albuquerque, New Mexico 87131"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Rick","family":"Lyons","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of New Mexico Health Sciences Center, Albuquerque, New Mexico 87131"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jimmy D.","family":"Ballard","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1046\/j.1365-2958.1997.1801552.x","volume":"23","year":"1997","unstructured":"Adler, E., A. Donella-Deana, F. Arigoni, L. A. Pinna, and P. Stragler. 1997. Structural relationship between a bacterial developmental protein and eukaryotic PP2C protein phosphatases. Mol. Microbiol. 23 : 57-62.","journal-title":"Mol. 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