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Although the incidence of<jats:italic>C. sordellii<\/jats:italic>infection is low, it is fatal in most cases. Since spore germination is believed to be the first step in the establishment of<jats:italic>Bacilli<\/jats:italic>and<jats:italic>Clostridia<\/jats:italic>infections, we analyzed the requirements for<jats:italic>C. sordellii<\/jats:italic>spore germination<jats:italic>in vitro<\/jats:italic>. Our data showed that<jats:italic>C. sordellii<\/jats:italic>spores require three structurally different amino acids and bicarbonate for maximum germination. Unlike the case for<jats:italic>Bacilli<\/jats:italic>species,<jats:sc>d<\/jats:sc>-alanine had no effect on<jats:italic>C. sordellii<\/jats:italic>spore germination.<jats:italic>C. sordellii<\/jats:italic>spores germinated only in a narrow pH range between 5.7 and 6.5. In contrast,<jats:italic>C. sordellii<\/jats:italic>spore germination was significantly less sensitive to temperature changes than that of the<jats:italic>Bacilli<\/jats:italic>. The analysis of the kinetics of<jats:italic>C. sordellii<\/jats:italic>spore germination showed strong allosteric behavior in the binding of<jats:sc>l<\/jats:sc>-phenylalanine and<jats:sc>l<\/jats:sc>-alanine but not in that of bicarbonate or<jats:sc>l<\/jats:sc>-arginine. By comparing germinant apparent binding affinities to their known<jats:italic>in vivo<\/jats:italic>concentrations, we postulated a mechanism for differential<jats:italic>C. sordellii<\/jats:italic>spore activation in the female reproductive tract.<\/jats:p>","DOI":"10.1128\/jb.01226-09","type":"journal-article","created":{"date-parts":[[2009,11,14]],"date-time":"2009-11-14T02:10:03Z","timestamp":1258164603000},"page":"418-425","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Requirements for Germination of<i>Clostridium sordellii<\/i>Spores<i>In Vitro<\/i>"],"prefix":"10.1128","volume":"192","author":[{"given":"Norma","family":"Ramirez","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Nevada, Las Vegas, Las Vegas, Nevada 89154"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernesto","family":"Abel-Santos","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Nevada, Las Vegas, Las Vegas, Nevada 89154"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1039\/b616695d","volume":"31","year":"2007","unstructured":"Abel-Santos, E., and T. 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