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Anthrax DnaB helicase (DnaB<jats:sub>BA<\/jats:sub>) is a 453-amino-acid, 50-kDa polypeptide with ATPase and DNA helicase activities. DnaB<jats:sub>BA<\/jats:sub>displayed distinct enzymatic and kinetic properties. DnaB<jats:sub>BA<\/jats:sub>has low single-stranded DNA (ssDNA)-dependent ATPase activity but possesses a strong 5\u2032\u21923\u2032 DNA helicase activity. The stimulation of ATPase activity appeared to be a function of the length of the ssDNA template rather than of ssDNA binding alone. The highest specific activity was observed with M13mp19 ssDNA. The results presented here indicated that the ATPase activity of DnaB<jats:sub>BA<\/jats:sub>was coupled to its migration on an ssDNA template rather than to DNA binding alone. It did not require nucleotide to bind ssDNA. DnaB<jats:sub>BA<\/jats:sub>demonstrated a strong DNA helicase activity that required ATP or dATP. Therefore, DnaB<jats:sub>BA<\/jats:sub>has an attenuated ATPase activity and a highly active DNA helicase activity. Based on the ratio of DNA helicase and ATPase activities, DnaB<jats:sub>BA<\/jats:sub>is highly efficient in DNA unwinding and its coupling to ATP consumption.<\/jats:p>","DOI":"10.1128\/jb.01259-08","type":"journal-article","created":{"date-parts":[[2008,10,18]],"date-time":"2008-10-18T00:59:46Z","timestamp":1224291586000},"page":"249-260","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["An Essential DnaB Helicase of<i>Bacillus anthracis<\/i>: Identification, Characterization, and Mechanism of Action"],"prefix":"10.1128","volume":"191","author":[{"given":"Esther E.","family":"Biswas","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084"},{"name":"Program in Biotechnology, Department of Bioscience Technologies, Thomas Jefferson University, Philadelphia, Pennsylvania 19107"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marjorie H.","family":"Barnes","sequence":"additional","affiliation":[{"name":"Microbiotix, Inc., Worcester, Massachusetts 01605"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald T.","family":"Moir","sequence":"additional","affiliation":[{"name":"Microbiotix, Inc., Worcester, Massachusetts 01605"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Subhasis B.","family":"Biswas","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"45476","DOI":"10.1074\/jbc.M308516200","volume":"278","year":"2003","unstructured":"Abhyankar, M. 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