{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:18:49Z","timestamp":1791425929688,"version":"4.3.3"},"reference-count":67,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Enterohemorrhagic<jats:italic>Escherichia coli<\/jats:italic>(EHEC) O157:H7 produces long bundles of polar type 4 pili (T4P) called HCP (for<jats:italic>h<\/jats:italic>emorrhagic<jats:italic>c<\/jats:italic>oli<jats:italic>p<\/jats:italic>ili) that form physical bridges between bacteria associating with human and animal epithelial cells. Here, we sought to further investigate whether HCP possessed other pathogenicity attributes associated with T4P production. Comparative studies performed with wild-type EHEC EDL933 and an isogenic<jats:italic>hcpA<\/jats:italic>mutant revealed that HCP play different roles in the biology of this organism. We found that in addition to promoting bacterial attachment to host cells, HCP mediate (i) invasion of epithelial cells, (ii) hemagglutination of rabbit erythrocytes, (iii) interbacterial connections conducive to biofilm formation, (iv) specific binding to host extracellular matrix proteins laminin and fibronectin but not collagen, and (v) twitching motility. Nonadherent laboratory<jats:italic>E. coli<\/jats:italic>strain HB101 complemented with<jats:italic>hcpABC<\/jats:italic>genes on plasmid pJX22, which specifies for HCP overproduction in EDL933, became hyperadherent and invasive and produced a thick biofilm, suggesting that the presence of HCP confers HB101(pJX22) new attributes otherwise not exhibited by HB101. Analogous to other bacteria in which T4P are involved in the pathogenesis of several infectious diseases, our data strongly suggest that HCP display multiple functions that may contribute to EHEC colonization of different hosts and to virulence, survival, and transmission of this food-borne pathogen.<\/jats:p>","DOI":"10.1128\/jb.01306-08","type":"journal-article","created":{"date-parts":[[2008,10,25]],"date-time":"2008-10-25T00:54:29Z","timestamp":1224896069000},"page":"411-421","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":92,"title":["The Type 4 Pili of Enterohemorrhagic<i>Escherichia coli<\/i>O157:H7 Are Multipurpose Structures with Pathogenic Attributes"],"prefix":"10.1128","volume":"191","author":[{"given":"Juan","family":"Xicohtencatl-Cortes","sequence":"first","affiliation":[{"name":"Department of Immunobiology, University of Arizona, 15101 N. Campbell Ave., Tucson, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vale\u0301rio","family":"Monteiro-Neto","sequence":"additional","affiliation":[{"name":"Department of Immunobiology, University of Arizona, 15101 N. Campbell Ave., Tucson, Arizona"},{"name":"Centro de Cie\u0302ncias da Sau\u0301de, Centro Universita\u0301rio do Maranha\u0303o, Sa\u0303o Lui\u0301s, Maranha\u0303o, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeus","family":"Saldan\u0303a","sequence":"additional","affiliation":[{"name":"Department of Immunobiology, University of Arizona, 15101 N. Campbell Ave., Tucson, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria A.","family":"Ledesma","sequence":"additional","affiliation":[{"name":"Department of Immunobiology, University of Arizona, 15101 N. Campbell Ave., Tucson, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Lui\u0301s","family":"Puente","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a Molecular, Instituto de Biotecnologi\u0301a, Universidad Nacional de Me\u0301xico, Cuernavaca, Me\u0301xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge A.","family":"Giro\u0301n","sequence":"additional","affiliation":[{"name":"Department of Immunobiology, University of Arizona, 15101 N. Campbell Ave., Tucson, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.tb02413.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1146\/annurev.micro.60.080805.142106","volume":"60","year":"2006","unstructured":"Barnhart, M. M., and M. R. Chapman. 2006. Curli biogenesis and function. Annu. Rev. Microbiol. 60 : 131-147.","journal-title":"Annu. Rev. Microbiol."},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1046\/j.1365-2672.2003.01931.x","volume":"94","year":"2003","unstructured":"Bechet, M., and R. Blondeau. 2003. Factors associated with the adherence and biofilm formation by Aeromonas caviae on glass surfaces. J. Appl. Microbiol. 94 : 1072-1078.","journal-title":"J. Appl. Microbiol."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1126\/science.280.5372.2114","volume":"280","year":"1998","unstructured":"Bieber, D., S. W. Ramer, C. Y. Wu, W. J. Murray, T. Tobe, R. Fernandez, and G. K. Schoolnik. 1998. Type IV pili, transient bacterial aggregates, and virulence of enteropathogenic Escherichia coli. Science 280 : 2114-2118.","journal-title":"Science"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.7.4447-4457.2001"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.11.3924-3929.1991"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1111\/j.1462-5822.2004.00372.x","volume":"6","year":"2004","unstructured":"Castaneda-Roldan, E. I., F. Avelino-Flores, M. Dall'Agnol, E. Freer, L. Cedillo, J. Dornand, and J. A. Giron. 2004. Adherence of Brucella to human epithelial cells and macrophages is mediated by sialic acid residues. Cell. Microbiol. 6 : 435-445.","journal-title":"Cell. Microbiol."},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1111\/j.1365-2958.1995.mmi_17061133.x","volume":"17","year":"1995","unstructured":"Chiang, S. L., R. K. Taylor, M. Koomey, and J. J. Mekalanos. 1995. Single amino acid substitutions in the N-terminus of Vibrio cholerae TcpA affect colonization, autoagglutination, and serum resistance. Mol. Microbiol. 17 : 1133-1142.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1038\/nrmicro885","volume":"2","year":"2004","unstructured":"Craig, L., M. E. Pique, and J. A. Tainer. 2004. Type IV pilus structure and bacterial pathogenicity. Nat. Rev. Microbiol. 2 : 363-378.","journal-title":"Nat. Rev. Microbiol."},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/0168-1605(94)00103-D","volume":"26","year":"1995","unstructured":"Dewanti, R., and A. C. Wong. 1995. Influence of culture conditions on biofilm formation by Escherichia coli O157:H7. Int. J. Food Microbiol. 26 : 147-164.","journal-title":"Int. J. Food Microbiol."},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"3427","DOI":"10.1111\/j.1365-2958.1992.tb02210.x","volume":"6","year":"1992","unstructured":"Donnenberg, M. S., J. A. Giron, J. P. Nataro, and J. B. Kaper. 1992. A plasmid-encoded type IV fimbrial gene of enteropathogenic Escherichia coli associated with localized adherence. Mol. Microbiol. 6 : 3427-3437.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/0076-6879(94)36030-8","volume":"236","year":"1994","unstructured":"Elsinghorst, E. A. 1994. Measurement of invasion by gentamicin resistance. Methods Enzymol. 236 : 405-420.","journal-title":"Methods Enzymol."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00464-07"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.23.2.336-346.1979"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02893-06"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1093\/infdis\/168.4.1037","volume":"168","year":"1993","unstructured":"Gir\u00f3n, J. A., M. S. Donnenberg, W. C. Martin, K. G. Jarvis, and J. B. Kaper. 1993. Distribution of the bundle-forming pilus structural gene (bfpA) among enteropathogenic Escherichia coli. J. Infect. Dis. 168 : 1037-1041.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1126\/science.1683004","volume":"254","year":"1991","unstructured":"Gir\u00f3n, J. A., A. S. Ho, and G. K. Schoolnik. 1991. An inducible bundle-forming pilus of enteropathogenic Escherichia coli. Science 254 : 710-713.","journal-title":"Science"},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1111\/j.1365-2958.1994.tb00996.x","volume":"12","year":"1994","unstructured":"Gir\u00f3n, J. A., M. M. Levine, and J. B. Kaper. 1994. Longus: a long pilus ultrastructure produced by human enterotoxigenic Escherichia coli. Mol. Microbiol. 12 : 71-82.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"705","DOI":"10.7326\/0003-4819-109-9-705","volume":"109","year":"1988","unstructured":"Griffin, P. M., S. M. Ostroff, R. V. Tauxe, K. D. Greene, J. G. Wells, J. H. Lewis, and P. A. Blake. 1988. Illnesses associated with Escherichia coli O157:H7 infections. A broad clinical spectrum. Ann. Intern. Med. 109 : 705-712.","journal-title":"Ann. Intern. Med."},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1111\/j.1462-5822.2005.00656.x","volume":"8","year":"2006","unstructured":"Guttman, J. A., Y. Li, M. E. Wickham, W. Deng, A. W. Vogl, and B. B. Finlay. 2006. Attaching and effacing pathogen-induced tight junction disruption in vivo. Cell. Microbiol. 8 : 634-645.","journal-title":"Cell. Microbiol."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro818"},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"119","DOI":"10.4142\/jvs.2004.5.2.119","volume":"5","year":"2004","unstructured":"Kim, S. H., and Y. H. Kim. 2004. Escherichia coli O157:H7 adherence to HEp-2 cells is implicated with curli expression and outer membrane integrity. J. Vet. Sci. 5 : 119-124.","journal-title":"J. Vet. Sci."},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1046\/j.1365-2958.2003.03525.x","volume":"48","year":"2003","unstructured":"Klausen, M., A. Heydorn, P. Ragas, L. Lambertsen, A. Aaes-Jorgensen, S. Molin, and T. Tolker-Nielsen. 2003. Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants. Mol. Microbiol. 48 : 1511-1524.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1046\/j.1365-2958.1999.01495.x","volume":"33","year":"1999","unstructured":"Knutton, S., R. K. Shaw, R. P. Anantha, M. S. Donnenberg, and A. A. Zorgani. 1999. The type IV bundle-forming pilus of enteropathogenic Escherichia coli undergoes dramatic alterations in structure associated with bacterial adherence, aggregation and dispersal. Mol. Microbiol. 33 : 499-509.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.4.2233-2244.2006"},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1111\/j.1462-2920.2006.00995.x","volume":"8","year":"2006","unstructured":"Low, A. S., N. Holden, T. Rosser, A. J. Roe, C. Constantinidou, J. L. Hobman, D. G. Smith, J. C. Low, and D. L. Gally. 2006. Analysis of fimbrial gene clusters and their expression in enterohaemorrhagic Escherichia coli O157:H7. Environ. Microbiol. 8 : 1033-1047.","journal-title":"Environ. Microbiol."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00435-06"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.5.3063-3071.2005"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.11.6262-6270.2004"},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"2803","DOI":"10.1093\/emboj\/19.12.2803","volume":"19","year":"2000","unstructured":"Martinez, J. J., M. A. Mulvey, J. D. Schilling, J. S. Pinkner, and S. J. Hultgren. 2000. Type 1 pilus-mediated bacterial invasion of bladder epithelial cells. EMBO J. 19 : 2803-2812.","journal-title":"EMBO J."},{"key":"e_1_3_2_33_2","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1146\/annurev.micro.56.012302.160938","volume":"56","year":"2002","unstructured":"Mattick, J. S. 2002. Type IV pili and twitching motility. Annu. Rev. Microbiol. 56 : 289-314.","journal-title":"Annu. Rev. Microbiol."},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.5.1664"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.5.2070-2074.1995"},{"key":"e_1_3_2_36_2","doi-asserted-by":"crossref","first-page":"S322","DOI":"10.1093\/clinids\/9.Supplement_4.S322","volume":"9","year":"1987","unstructured":"McKeown-Longo, P. J. 1987. Fibronectin-cell surface interactions. Rev. Infect. Dis. 9(Suppl. 4): S322-S334.","journal-title":"Rev. Infect. Dis."},{"key":"e_1_3_2_37_2","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1038\/35024105","volume":"407","year":"2000","unstructured":"Merz, A. J., M. So, and M. P. Sheetz. 2000. Pilus retraction powers bacterial twitching motility. Nature 407 : 98-102.","journal-title":"Nature"},{"key":"e_1_3_2_38_2","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.ijmm.2005.07.010","volume":"295","year":"2005","unstructured":"Naylor, S. W., D. L. Gally, and J. C. Low. 2005. Enterohaemorrhagic E. coli in veterinary medicine. Int. J. Med. Microbiol. 295 : 419-441.","journal-title":"Int. J. Med. Microbiol."},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1046\/j.1365-2958.2000.01690.x","volume":"35","year":"2000","unstructured":"Nicholls, L., T. H. Grant, and R. M. Robins-Browne. 2000. Identification of a novel genetic locus that is required for in vitro adhesion of a clinical isolate of enterohaemorrhagic Escherichia coli to epithelial cells. Mol. Microbiol. 35 : 275-288.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.11.5142-5150.1994"},{"key":"e_1_3_2_41_2","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1046\/j.1365-2958.1998.01062.x","volume":"30","year":"1998","unstructured":"O'Toole, G. A., and R. Kolter. 1998. Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development. Mol. Microbiol. 30 : 295-304.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.3.1411-1422.2005"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.11.6999-7009.2001"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.3.450"},{"key":"e_1_3_2_45_2","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1038\/35054089","volume":"409","year":"2001","unstructured":"Perna, N. T., G. Plunkett, 3rd, V. Burland, B. Mau, J. D. Glasner, D. J. Rose, G. F. Mayhew, P. S. Evans, J. Gregor, H. A. Kirkpatrick, G. Posfai, J. Hackett, S. Klink, A. Boutin, Y. Shao, L. Miller, E. J. Grotbeck, N. W. Davis, A. Lim, E. T. Dimalanta, K. D. Potamousis, J. Apodaca, T. S. Anantharaman, J. Lin, G. Yen, D. C. Schwartz, R. A. Welch, and F. R. Blattner. 2001. Genome sequence of enterohaemorrhagic Escherichia coli O157:H7. Nature 409 : 529-533.","journal-title":"Nature"},{"key":"e_1_3_2_46_2","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/j.cell.2006.02.012","volume":"124","year":"2006","unstructured":"Pizarro-Cerda, J., and P. Cossart. 2006. Bacterial adhesion and entry into host cells. Cell 124 : 715-727.","journal-title":"Cell"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.16.4727-4736.2001"},{"key":"e_1_3_2_48_2","doi-asserted-by":"crossref","first-page":"4017","DOI":"10.1073\/pnas.96.7.4017","volume":"96","year":"1999","unstructured":"Pujol, C., E. Eugene, M. Marceau, and X. Nassif. 1999. The meningococcal PilT protein is required for induction of intimate attachment to epithelial cells following pilus-mediated adhesion. Proc. Natl. Acad. Sci. USA 96 : 4017-4022.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.10.3551-3555.2005"},{"key":"e_1_3_2_50_2","doi-asserted-by":"crossref","first-page":"10637","DOI":"10.1073\/pnas.0704104104","volume":"104","year":"2007","unstructured":"Rendon, M. A., Z. Saldana, A. L. Erdem, V. Monteiro-Neto, A. Vazquez, J. B. Kaper, J. L. Puente, and J. A. Giron. 2007. Commensal and pathogenic Escherichia coli use a common pilus adherence factor for epithelial cell colonization. Proc. Natl. Acad. Sci. USA 104 : 10637-10642.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_51_2","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1056\/NEJM198303243081203","volume":"308","year":"1983","unstructured":"Riley, L. W., R. S. Remis, S. D. Helgerson, H. B. McGee, J. G. Wells, B. R. Davis, R. J. Hebert, E. S. Olcott, L. M. Johnson, N. T. Hargrett, P. A. Blake, and M. L. Cohen. 1983. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N. Engl. J. Med. 308 : 681-685.","journal-title":"N. Engl. J. Med."},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.3.848-854.2000"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.10.2221"},{"key":"e_1_3_2_54_2","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1046\/j.1365-2958.2001.02512.x","volume":"41","year":"2001","unstructured":"Sheikh, J., S. Hicks, M. Dall'Agnol, A. D. Phillips, and J. P. Nataro. 2001. Roles for Fis and YafK in biofilm formation by enteroaggregative Escherichia coli. Mol. Microbiol. 41 : 983-997.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.55.8.1824-1829.1987"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.2.1255-1265.2006"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.6.3094-3100.2002"},{"key":"e_1_3_2_58_2","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1146\/annurev.mi.47.100193.003025","volume":"47","year":"1993","unstructured":"Strom, M. S., and S. Lory. 1993. Structure-function and biogenesis of the type IV pili. Annu. Rev. Microbiol. 47 : 565-596.","journal-title":"Annu. Rev. Microbiol."},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.3.1400-1407.2000"},{"key":"e_1_3_2_60_2","first-page":"1073","volume":"365","year":"2005","unstructured":"Tarr, P. I., C. A. Gordon, and W. L. Chandler. 2005. Shiga-toxin-producing Escherichia coli and haemolytic uraemic syndrome. Lancet 365 : 1073-1086.","journal-title":"Lancet"},{"key":"e_1_3_2_61_2","doi-asserted-by":"crossref","first-page":"2833","DOI":"10.1073\/pnas.84.9.2833","volume":"84","year":"1987","unstructured":"Taylor, R. K., V. L. Miller, D. B. Furlong, and J. J. Mekalanos. 1987. Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin. Proc. Natl. Acad. Sci. USA 84 : 2833-2837.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.10.5416-5427.2002"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.9.4985-4995.2003"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.1.395-399.2002"},{"key":"e_1_3_2_65_2","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1046\/j.1365-2958.1999.01624.x","volume":"34","year":"1999","unstructured":"Watnick, P. I., and R. Kolter. 1999. Steps in the development of a Vibrio cholerae El Tor biofilm. Mol. Microbiol. 34 : 586-595.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.1.313-316.1997"},{"key":"e_1_3_2_67_2","doi-asserted-by":"crossref","first-page":"3519","DOI":"10.1172\/JCI30727","volume":"117","year":"2007","unstructured":"Xicohtencatl-Cortes, J., V. Monteiro-Neto, M. A. Ledesma, D. M. Jordan, O. Francetic, J. B. Kaper, J. L. Puente, and J. A. Giron. 2007. Intestinal adherence associated with type IV pili of enterohemorrhagic Escherichia coli O157:H7. J. Clin. Investig. 117 : 3519-3529.","journal-title":"J. Clin. Investig."},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.9.5389-5393.2003"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.01306-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.01306-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,2]],"date-time":"2024-03-02T00:42:08Z","timestamp":1709340128000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.01306-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1]]},"references-count":67,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2009,1]]}},"alternative-id":["10.1128\/JB.01306-08"],"URL":"https:\/\/doi.org\/10.1128\/jb.01306-08","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,1]]},"assertion":[{"value":"2008-09-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-10-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}