{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T12:07:38Z","timestamp":1787141258307,"version":"build-2736575974"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2008,6,15]],"date-time":"2008-06-15T00:00:00Z","timestamp":1213488000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2008,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The DevR-DevS two-component system of\n            <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n            mediates bacterial adaptation to hypoxia, a condition believed to be associated with the initiation and maintenance of dormant bacilli during latent tuberculosis. The activity of the Rv3134c-\n            <jats:italic>devRS<\/jats:italic>\n            operon was studied in\n            <jats:italic>M. tuberculosis<\/jats:italic>\n            using several transcriptional fusions comprised of promoter regions and the\n            <jats:italic>gfp<\/jats:italic>\n            reporter gene under inducing and aerobic conditions. Aerobic transcription was DevR independent, while hypoxic induction was completely DevR dependent. The hypoxia transcriptional start point, T\n            <jats:sub>H<\/jats:sub>\n            , was mapped at \u221240 bp upstream of Rv3134c. In contrast, the divergently transcribed Rv3135 gene was not induced under hypoxic conditions. DNase I footprinting and mutational analyses demonstrated that induction required the interaction of DevR\u223cP with binding sites centered at bp \u221242.5 and \u221263.5 relative to T\n            <jats:sub>H<\/jats:sub>\n            . Binding to the distal site (D) was necessary to recruit another molecule of DevR\u223cP to the proximal site (P), and interaction with both sequences was essential for promoter activation. These sites did not bind to either unphosphorylated or phosphorylation-defective DevR protein, which was consistent with an essential role for DevR\u223cP in activation. Phosphorylated DevR also bound to three copies of the motif at the\n            <jats:italic>hspX<\/jats:italic>\n            promoter. The Rv3134c and\n            <jats:italic>hspX<\/jats:italic>\n            promoters have a similar architecture, wherein the proximal DevR\u223cP binding site overlaps with the promoter \u221235 element. A model for the likely mode of action of DevR at these promoters is discussed.\n          <\/jats:p>","DOI":"10.1128\/jb.01308-07","type":"journal-article","created":{"date-parts":[[2008,3,22]],"date-time":"2008-03-22T00:34:02Z","timestamp":1206146042000},"page":"4301-4312","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":81,"title":["Cooperative Binding of Phosphorylated DevR to Upstream Sites Is Necessary and Sufficient for Activation of the Rv3134c-\n            <i>devRS<\/i>\n            Operon in\n            <i>Mycobacterium tuberculosis<\/i>\n            : Implication in the Induction of DevR Target Genes"],"prefix":"10.1128","volume":"190","author":[{"given":"Santosh","family":"Chauhan","sequence":"first","affiliation":[{"name":"Department of Biotechnology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110029, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaya Sivaswami","family":"Tyagi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110029, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"4245","DOI":"10.1093\/nar\/gkl521","volume":"34","year":"2006","unstructured":"Agarwal, N., and A. 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