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Among the numerous extracellular factors that<jats:italic>S. marcescens<\/jats:italic>is able to produce, the PhlA phospholipase is the only described exoprotein secreted by the flagellar apparatus while simultaneously being a member of the flagellar regulon. To gain insight into the regulatory mechanism that couples PhlA and flagellar expression, we conducted a generalized insertional mutagenesis and screened for PhlA-deficient strains. We found that three independent mutations in the<jats:italic>wec<\/jats:italic>cluster, which impaired the assembly of enterobacterial common antigen (ECA), provoked the inhibition of PhlA expression. Swimming and swarming assays showed that in these strains, motility was severely affected. Microscopic examination and flagellin immunodetection demonstrated that a strong defect in flagellum expression was responsible for the reduced motility in the<jats:italic>wec<\/jats:italic>mutant strains. Furthermore, we determined that in the ECA-defective strains, the transcriptional cascade that controls flagellar assembly was turned off due to the down-regulation of<jats:italic>flhDC<\/jats:italic>expression. These findings provide a new perspective on the physiological role of the ECA, providing evidence that in<jats:italic>S. marcescens<\/jats:italic>, its biosynthesis conditions the expression of the flagellar regulon.<\/jats:p>","DOI":"10.1128\/jb.01348-07","type":"journal-article","created":{"date-parts":[[2007,11,3]],"date-time":"2007-11-03T00:46:02Z","timestamp":1194050762000},"page":"213-220","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":47,"title":["Enterobacterial Common Antigen Integrity Is a Checkpoint for Flagellar Biogenesis in<i>Serratia marcescens<\/i>"],"prefix":"10.1128","volume":"190","author":[{"given":"Mari\u0301a E.","family":"Castelli","sequence":"first","affiliation":[{"name":"Instituto de Biologi\u0301a Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas, Departamento de Microbiologi\u0301a, Facultad de Ciencias Bioqui\u0301micas y Farmace\u0301uticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Griselda V.","family":"Fedrigo","sequence":"additional","affiliation":[{"name":"Instituto de Biologi\u0301a Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas, Departamento de Microbiologi\u0301a, Facultad de Ciencias Bioqui\u0301micas y Farmace\u0301uticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana L.","family":"Clementi\u0301n","sequence":"additional","affiliation":[{"name":"Instituto de Biologi\u0301a Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas, Departamento de Microbiologi\u0301a, Facultad de Ciencias Bioqui\u0301micas y Farmace\u0301uticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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