{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T12:16:44Z","timestamp":1791461804618,"version":"4.3.4"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2014,4,15]],"date-time":"2014-04-15T00:00:00Z","timestamp":1397520000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2014,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The ATP-dependent transporter gene\n            <jats:italic>abcA<\/jats:italic>\n            in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Staphylococcus aureus<\/jats:named-content>\n            confers resistance to hydrophobic \u03b2-lactams. In strain ISP794,\n            <jats:italic>abcA<\/jats:italic>\n            is regulated by the transcriptional regulators MgrA and NorG and shares a 420-nucleotide intercistronic region with the divergently transcribed\n            <jats:italic>pbp4<\/jats:italic>\n            gene, which encodes the transpeptidase Pbp4. Exposure of exponentially growing cells to iron-limited media, oxidative stress, and acidic pH (5.5) for 0.5 to 2 h had no effect on\n            <jats:italic>abcA<\/jats:italic>\n            expression. In contrast, nutrient limitation produced a significant increase in\n            <jats:italic>abcA<\/jats:italic>\n            transcripts. We identified three additional regulators (SarA, SarZ, and Rot) that bind to the overlapping promoter region of\n            <jats:italic>abcA<\/jats:italic>\n            and\n            <jats:italic>pbp4<\/jats:italic>\n            in strain MW2 and investigated their role in the regulation of\n            <jats:italic>abcA<\/jats:italic>\n            expression. Expression of\n            <jats:italic>abcA<\/jats:italic>\n            is decreased by 10.0-fold\n            <jats:italic>in vivo<\/jats:italic>\n            in a subcutaneous abscess model.\n            <jats:italic>In vitro<\/jats:italic>\n            ,\n            <jats:italic>abcA<\/jats:italic>\n            expression depends on\n            <jats:italic>rot<\/jats:italic>\n            and\n            <jats:italic>sarZ<\/jats:italic>\n            regulators. Moenomycin A exposure of strain MW2 produced an increase in\n            <jats:italic>abcA<\/jats:italic>\n            transcripts. Relative to MW2, the MIC of moenomycin was decreased 8-fold for MW2\u0394\n            <jats:italic>abcA<\/jats:italic>\n            and increased 10-fold for the MW2\n            <jats:italic>abcA<\/jats:italic>\n            overexpresser, suggesting that moenomycin is a substrate of AbcA.\n          <\/jats:p>","DOI":"10.1128\/jb.01406-13","type":"journal-article","created":{"date-parts":[[2014,2,8]],"date-time":"2014-02-08T07:34:20Z","timestamp":1391844860000},"page":"1532-1539","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["Regulation of Expression of\n            <i>abcA<\/i>\n            and Its Response to Environmental Conditions"],"prefix":"10.1128","volume":"196","author":[{"given":"Regis A.","family":"Villet","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases and Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Que Chi","family":"Truong-Bolduc","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin","family":"Wang","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoe","family":"Estabrooks","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heidi","family":"Medeiros","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David C.","family":"Hooper","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa055356"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1106.040641"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.298.15.1763"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000181727.04501.F3"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2006.04.005"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.64.4.672-693.2000"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2008\/021188-0"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks035"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.033167-0"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00018-10"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.3.1104-1107.2006"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00655-08"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/29.3.245"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.1.161-169.2005"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(96)00541-0"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.7.2395-2405.2005"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(96)82965-9"},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1111\/j.1432-1033.1995.tb20834.x","article-title":"Nucleotide sequence of the lantibiotic Pep5 biosynthetic gene cluster and functional analysis of PepP and PepC. 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