{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T06:44:15Z","timestamp":1787985855238,"version":"build-2784847793"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T00:00:00Z","timestamp":1301616000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Bacterial citrus canker disease, which is caused by\n            <jats:italic>Xanthomonas citri<\/jats:italic>\n            subsp.\n            <jats:italic>citri<\/jats:italic>\n            , is one of the most devastating diseases of citrus plants. In this study, we characterized the role of the two-component regulatory system ColR\/ColS in the pathogenicity of\n            <jats:italic>X. citri<\/jats:italic>\n            subsp.\n            <jats:italic>citri. colS<\/jats:italic>\n            mutants (256A10 and 421E7),\n            <jats:italic>colR<\/jats:italic>\n            mutants (386C6 and 417E10), and a\n            <jats:italic>colR colS<\/jats:italic>\n            double mutant (306DSR) all lost pathogenicity and produced no symptoms on grapefruit leaves inoculated by either pressure infiltration or the spray method. The pathogenicity defect of the\n            <jats:italic>colS<\/jats:italic>\n            ,\n            <jats:italic>colR<\/jats:italic>\n            , and\n            <jats:italic>colR colS<\/jats:italic>\n            mutants could be complemented using the wild-type\n            <jats:italic>colS<\/jats:italic>\n            ,\n            <jats:italic>colR<\/jats:italic>\n            , and\n            <jats:italic>colR colS<\/jats:italic>\n            genes, respectively. Mutation of\n            <jats:italic>colS<\/jats:italic>\n            or\n            <jats:italic>colR<\/jats:italic>\n            significantly reduced\n            <jats:italic>X. citri<\/jats:italic>\n            subsp.\n            <jats:italic>citri<\/jats:italic>\n            growth\n            <jats:italic>in planta<\/jats:italic>\n            . The ColR\/ColS system also played important roles in bacterial biofilm formation in glass tubes and on leaf surfaces, lipopolysaccharide (LPS) production, catalase activity, and tolerance of environmental stress, including phenol, copper, and hydrogen peroxide. Furthermore, quantitative reverse transcription-PCR assays demonstrated that the ColR\/ColS system positively regulated the expression of important virulence genes, including\n            <jats:italic>hrpD<\/jats:italic>\n            6,\n            <jats:italic>hpaF<\/jats:italic>\n            , the O-antigen LPS synthesis gene\n            <jats:italic>rfbC<\/jats:italic>\n            , and the catalase gene\n            <jats:italic>katE<\/jats:italic>\n            . Overall, our data indicate that the two-component regulatory system ColR\/ColS is critical for\n            <jats:italic>X. citri<\/jats:italic>\n            subsp.\n            <jats:italic>citri<\/jats:italic>\n            virulence, growth\n            <jats:italic>in planta<\/jats:italic>\n            , biofilm formation, catalase activity, LPS production, and resistance to environmental stress.\n          <\/jats:p>","DOI":"10.1128\/jb.01415-10","type":"journal-article","created":{"date-parts":[[2011,1,22]],"date-time":"2011-01-22T04:44:31Z","timestamp":1295671471000},"page":"1590-1599","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":62,"title":["The ColR\/ColS Two-Component System Plays Multiple Roles in the Pathogenicity of the Citrus Canker Pathogen\n            <i>Xanthomonas citri<\/i>\n            subsp.\n            <i>citri<\/i>"],"prefix":"10.1128","volume":"193","author":[{"given":"Qing","family":"Yan","sequence":"first","affiliation":[{"name":"Citrus Research and Education Center, Department of Microbiology and Cell Science, University of Florida, 700 Experiment Station Road, Lake Alfred, Florida 33850"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nian","family":"Wang","sequence":"additional","affiliation":[{"name":"Citrus Research and Education Center, Department of Microbiology and Cell Science, University of Florida, 700 Experiment Station Road, Lake Alfred, Florida 33850"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1146\/annurev.py.33.090195.001503","volume":"33","year":"1995","unstructured":"Baker, C., and E. 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