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Hence, the cell envelope of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Rhizobium leguminosarum<\/jats:named-content>\n            is critical to cell survival under both free-living and symbiotic conditions. Transposon mutagenesis of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">R. leguminosarum<\/jats:named-content>\n            strain 3841 followed by a screen to isolate mutants with defective cell envelopes led to the identification of a novel conserved operon (RL3499-RL3502) consisting of a putative\n            <jats:italic>moxR<\/jats:italic>\n            -like AAA\n            <jats:sup>+<\/jats:sup>\n            ATPase, a hypothetical protein with a domain of unknown function (designated domain of unknown function 58), and two hypothetical transmembrane proteins. Mutation of genes within this operon resulted in increased sensitivity to membrane-disruptive agents such as detergents, hydrophobic antibiotics, and alkaline pH. On minimal media, the mutants retain their rod shape but are roughly 3 times larger than the wild type. On media containing glycine or peptides such as yeast extract, the mutants form large, distorted spheres and are incapable of sustained growth under these culture conditions. Expression of the operon is maximal during the stationary phase of growth and is reduced in a\n            <jats:italic>chvG<\/jats:italic>\n            mutant, indicating a role for this sensor kinase in regulation of the operon. Our findings provide the first functional insight into these genes of unknown function, suggesting a possible role in cell envelope development in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Rhizobium leguminosarum<\/jats:named-content>\n            . Given the broad conservation of these genes among the\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Alphaproteobacteria<\/jats:named-content>\n            , the results of this study may also provide insight into the physiological role of these genes in other\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Alphaproteobacteria<\/jats:named-content>\n            , including the animal pathogen\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/jb.01456-10","type":"journal-article","created":{"date-parts":[[2011,2,26]],"date-time":"2011-02-26T05:49:03Z","timestamp":1298699343000},"page":"2684-2694","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Mutation of a Broadly Conserved Operon (RL3499-RL3502) from Rhizobium leguminosarum Biovar\n            <i>viciae<\/i>\n            Causes Defects in Cell Morphology and Envelope Integrity"],"prefix":"10.1128","volume":"193","author":[{"given":"Elizabeth M.","family":"Vanderlinde","sequence":"first","affiliation":[{"name":"Department of Biology, University of Regina, 3737 Wascana Parkway, Regina, Saskatchewan, Canada S4S 0A2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samantha A.","family":"Magnus","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Regina, 3737 Wascana Parkway, Regina, Saskatchewan, Canada S4S 0A2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dinah D.","family":"Tambalo","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Calgary, 2500 University Drive, N.W., Calgary, Alberta, Canada T2N 1N4"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan F.","family":"Koval","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada N6A 5C1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher K.","family":"Yost","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Regina, 3737 Wascana Parkway, Regina, Saskatchewan, Canada S4S 0A2"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_3_3_2","doi-asserted-by":"crossref","first-page":"2015","DOI":"10.1271\/bbb.58.2015","article-title":"Hyper-autolysis of the facultative alkaliphile Bacillus sp. 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