{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T02:31:12Z","timestamp":1791167472007,"version":"4.1.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2009,7,1]],"date-time":"2009-07-01T00:00:00Z","timestamp":1246406400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Lipopolysaccharide (LPS) is a major constituent of the outer membrane of gram-negative bacteria that serves as a barrier against harmful molecules, including antibiotics. The\n            <jats:italic>waaYZ<\/jats:italic>\n            locus that encodes the LPS core biosynthetic function in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            was found to be induced strongly by superoxide generators but not by H\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>2<\/jats:sub>\n            , ethanol, or heat shock. This induction was dependent on SoxRS, a superoxide and nitric oxide sensing system, through a soxbox in the\n            <jats:italic>waaY<\/jats:italic>\n            promoter that binds SoxS. A \u0394\n            <jats:italic>waaYZ<\/jats:italic>\n            mutant became more sensitive to some superoxide generators, and the activation of SoxR by these drugs became more sensitized in the mutant. Through phenotypic microarray analysis, we found that the mutant became sensitive to a wide variety of chemicals not restricted to oxidizing agents. We found that the mutant is under envelope stress and is altered in LPS composition, as monitored by the level of \u03c3\n            <jats:sup>E<\/jats:sup>\n            activation and changes in the electrophoretic mobility of LPS, respectively.\n            <jats:italic>waaY<\/jats:italic>\n            expression was also regulated by MarA (multiple-antibiotic resistance regulator), which shares a binding site (soxbox) with SoxS, and was induced by salicylate, a nonoxidative compound. These results demonstrate a novel way of protecting gram-negative bacteria against various compounds by modifying LPS, possibly through phosphorylation. Since either oxidant or nonoxidant compounds elicit resistance toward themselves and other toxic drugs, this mechanism could serve as an efficient way for pathogenic bacteria to enhance survival during antibiotic treatment within an oxidant-rich host immune environment.\n          <\/jats:p>","DOI":"10.1128\/jb.01474-08","type":"journal-article","created":{"date-parts":[[2009,4,18]],"date-time":"2009-04-18T01:07:23Z","timestamp":1240016843000},"page":"4441-4450","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["SoxRS-Mediated Lipopolysaccharide Modification Enhances Resistance against Multiple Drugs in\n            <i>Escherichia coli<\/i>"],"prefix":"10.1128","volume":"191","author":[{"given":"Joon-Hee","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Pharmacy, College of Pharmacy, Pusan National University, Busan 609-735, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang-Lok","family":"Lee","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won-Sik","family":"Yeo","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su-Jin","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, College of Pharmacy, Pusan National University, Busan 609-735, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung-Hye","family":"Roe","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1111\/j.1365-2958.2003.03982.x","volume":"52","year":"2004","unstructured":"Alba, B. 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