{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:23:45Z","timestamp":1790738625936,"version":"4.1.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2014,5,15]],"date-time":"2014-05-15T00:00:00Z","timestamp":1400112000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2014,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Mycobacterium tuberculosis<\/jats:named-content>\n            expresses the 28-kDa protein HupB (Rv2986c) and the Fe\n            <jats:sup>3+<\/jats:sup>\n            -specific high-affinity siderophores mycobactin and carboxymycobactin upon iron limitation. The objective of this study was to understand the functional role of HupB in iron acquisition. A\n            <jats:italic>hupB<\/jats:italic>\n            mutant strain of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. tuberculosis<\/jats:named-content>\n            , subjected to growth in low-iron medium (0.02 \u03bcg Fe ml\n            <jats:sup>\u22121<\/jats:sup>\n            ), showed a marked reduction of both siderophores with low transcript levels of the\n            <jats:italic>mbt<\/jats:italic>\n            genes encoding the MB biosynthetic machinery. Complementation of the mutant strain with\n            <jats:italic>hupB<\/jats:italic>\n            restored siderophore production to levels comparable to that of the wild type. We demonstrated the binding of HupB to the\n            <jats:italic>mbtB<\/jats:italic>\n            promoter by both electrophoretic mobility shift assays and DNA footprinting. The latter revealed the HupB binding site to be a 10-bp AT-rich region. While negative regulation of the\n            <jats:italic>mbt<\/jats:italic>\n            machinery by IdeR is known, this is the first report of positive regulation of the\n            <jats:italic>mbt<\/jats:italic>\n            operon by HupB. Interestingly, the mutant strain failed to survive inside macrophages, suggesting that HupB plays an important role\n            <jats:italic>in vivo<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jb.01483-13","type":"journal-article","created":{"date-parts":[[2014,3,8]],"date-time":"2014-03-08T05:43:34Z","timestamp":1394257414000},"page":"1853-1865","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":68,"title":["Iron-Regulated Protein HupB of Mycobacterium tuberculosis Positively Regulates Siderophore Biosynthesis and Is Essential for Growth in Macrophages"],"prefix":"10.1128","volume":"196","author":[{"given":"Satya Deo","family":"Pandey","sequence":"first","affiliation":[{"name":"Department of Animal Sciences, University of Hyderabad, Hyderabad, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitali","family":"Choudhury","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, University of Hyderabad, Hyderabad, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suhail","family":"Yousuf","sequence":"additional","affiliation":[{"name":"Laboratory of Computational and Functional Genomics, Centre for DNA Fingerprinting and Diagnostics, Hyderabad, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul R.","family":"Wheeler","sequence":"additional","affiliation":[{"name":"Tuberculosis Research Group, Animal Health and Veterinary Laboratories Agency, Belfield, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen V.","family":"Gordon","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and UCD Conway Institute, University College Dublin, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akash","family":"Ranjan","sequence":"additional","affiliation":[{"name":"Laboratory of Computational and Functional Genomics, Centre for DNA Fingerprinting and Diagnostics, Hyderabad, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manjula","family":"Sritharan","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, University of Hyderabad, Hyderabad, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2003.08.012"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1517\/13543784.12.5.841"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009227206890"},{"key":"e_1_3_3_5_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-3-642-65502-9_1","article-title":"The role of iron in bacterial infections, with special consideration of host-tubercle bacillus interaction","volume":"60","author":"Kochan I","year":"1973","unstructured":"KochanI. 1973. The role of iron in bacterial infections, with special consideration of host-tubercle bacillus interaction. Curr. Top. Microbiol. Immunol. 60:1\u201330.","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.54.1.881"},{"key":"e_1_3_3_7_2","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0255-0857(21)02343-4","article-title":"Iron and bacterial virulence","volume":"24","author":"Sritharan M","year":"2006","unstructured":"SritharanM. 2006. Iron and bacterial virulence. Indian J. Med. Microbiol. 24:163\u2013164. http:\/\/www.ijmm.org\/text.asp?2006\/24\/3\/163\/26987.","journal-title":"Indian J. Med. Microbiol."},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.12.6.1242-1251.1975"},{"key":"e_1_3_3_9_2","first-page":"341","volume-title":"Tuberculosis and the tubercle bacillus","author":"Quadri LE","year":"2005","unstructured":"QuadriLERatledgeC. 2005. Iron metabolism in the tubercle bacillus and other mycobacteria, p 341\u2013357. In ColeSTEisenachKDMcMurrayDNJacobsWRJ (ed), Tuberculosis and the tubercle bacillus. 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