{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T14:53:15Z","timestamp":1791471195042,"version":"4.3.4"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2007,2,1]],"date-time":"2007-02-01T00:00:00Z","timestamp":1170288000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The core lipopolysaccharide (LPS) of\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            contains two galacturonic acid (GalA) residues, but only one GalA transferase (WabG) has been identified. Data from chemical and structural analysis of LPS isolated from a\n            <jats:italic>wabO<\/jats:italic>\n            mutant show the absence of the inner core \u03b2-GalA residue linked to\n            <jats:sc>l<\/jats:sc>\n            -\n            <jats:italic>glycero<\/jats:italic>\n            -\n            <jats:sc>d<\/jats:sc>\n            -\n            <jats:italic>manno<\/jats:italic>\n            -heptose III (\n            <jats:sc>l<\/jats:sc>\n            ,\n            <jats:sc>d<\/jats:sc>\n            -Hep III). An in vitro assay demonstrates that the purified WabO is able to catalyze the transfer of GalA from UDP-GalA to the acceptor LPS isolated from the\n            <jats:italic>wabO<\/jats:italic>\n            mutant, but not to LPS isolated from\n            <jats:italic>waaQ<\/jats:italic>\n            mutant (deficient in\n            <jats:sc>l<\/jats:sc>\n            ,\n            <jats:sc>d<\/jats:sc>\n            -Hep III). The absence of this inner core \u03b2-GalA residue results in a decrease in virulence in a capsule-dependent experimental mouse pneumonia model. In addition, this mutation leads to a strong reduction in cell-bound capsule. Interestingly, a K66\n            <jats:italic>Klebsiella<\/jats:italic>\n            strain (natural isolate) without a functional\n            <jats:italic>wabO<\/jats:italic>\n            gene shows reduced levels of cell-bound capsule in comparison to those of other K66 strains. Thus, the WabO enzyme plays an important role in core LPS biosynthesis and determines the level of cell-bound capsule in\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jb.01489-06","type":"journal-article","created":{"date-parts":[[2006,12,2]],"date-time":"2006-12-02T01:35:11Z","timestamp":1165023311000},"page":"1128-1137","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["A Second Galacturonic Acid Transferase Is Required for Core Lipopolysaccharide Biosynthesis and Complete Capsule Association with the Cell Surface in\n            <i>Klebsiella pneumoniae<\/i>"],"prefix":"10.1128","volume":"189","author":[{"given":"Sandra","family":"Fresno","sequence":"first","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Facultad de Biologi\u0301a, Universidad de Barcelona, Diagonal 645, 08071 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Jime\u0301nez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Facultad de Biologi\u0301a, Universidad de Barcelona, Diagonal 645, 08071 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roci\u0301o","family":"Canals","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Facultad de Biologi\u0301a, Universidad de Barcelona, Diagonal 645, 08071 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Merino","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Facultad de Biologi\u0301a, Universidad de Barcelona, Diagonal 645, 08071 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Michela","family":"Corsaro","sequence":"additional","affiliation":[{"name":"Dipartimento di Chimica Organica e Biochimica, Universita\u0300 Federico II di Napoli, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Lanzetta","sequence":"additional","affiliation":[{"name":"Dipartimento di Chimica Organica e Biochimica, Universita\u0300 Federico II di Napoli, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelangelo","family":"Parrilli","sequence":"additional","affiliation":[{"name":"Dipartimento di Chimica Organica e Biochimica, Universita\u0300 Federico II di Napoli, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppina","family":"Pieretti","sequence":"additional","affiliation":[{"name":"Dipartimento di Chimica Organica e Biochimica, Universita\u0300 Federico II di Napoli, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Regue\u0301","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Parasitologi\u0301a Sanitarias, Facultad de Farmacia, Universidad de Barcelona, Av. Joan XXIII s\/n, Barcelona 08028, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan M.","family":"Toma\u0301s","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Facultad de Biologi\u0301a, Universidad de Barcelona, Diagonal 645, 08071 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-6215(84)85242-8"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0923-2508(97)87644-9","volume":"148","year":"1997","unstructured":"Climent, N., S. Ferrer, X. Rubires, S. Merino, J. 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