{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T04:18:36Z","timestamp":1790309916195,"version":"4.1.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2010,6,15]],"date-time":"2010-06-15T00:00:00Z","timestamp":1276560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2010,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The gene encoding resistance to methicillin and other \u03b2-lactam antibiotics in staphylococci,\n            <jats:italic>mecA<\/jats:italic>\n            , is carried on a genomic island, SCC\n            <jats:italic>mec<\/jats:italic>\n            (for staphylococcal cassette chromosome\n            <jats:italic>mec<\/jats:italic>\n            ). The chromosomal excision and integration of types I to IV SCC\n            <jats:italic>mec<\/jats:italic>\n            are catalyzed by the site-specific recombinases CcrA and CcrB, the genes for which are encoded on each element. We sought to identify the relative contributions of CcrA and CcrB in the excision and integration of SCC\n            <jats:italic>mec<\/jats:italic>\n            . Purified CcrB but not CcrA was shown to mediate the gel shift of chromosomal target integration sequences (\n            <jats:italic>attB<\/jats:italic>\n            ) in electrophoretic mobility shift assays. However, preincubation of CcrB-DNA complexes with increasing concentrations of CcrA blocked gel shift. The interaction of CcrB and CcrA was confirmed by\n            <jats:italic>Escherichia coli<\/jats:italic>\n            two-hybrid analysis. SCC\n            <jats:italic>mec<\/jats:italic>\n            excision mediated by plasmid-encoded and inducible\n            <jats:italic>ccrA<\/jats:italic>\n            ,\n            <jats:italic>ccrB<\/jats:italic>\n            , or both genes was assessed by PCR in\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            . CcrB alone could mediate excision but excision was at an alternate\n            <jats:italic>att<\/jats:italic>\n            site (\n            <jats:italic>attR2<\/jats:italic>\n            ) within the right extremity of SCC\n            <jats:italic>mec<\/jats:italic>\n            . In contrast, both CcrB and CcrA were required to mediate excision at the chromosomal\n            <jats:italic>attB<\/jats:italic>\n            site (called\n            <jats:italic>attR<\/jats:italic>\n            when SCC\n            <jats:italic>mec<\/jats:italic>\n            is integrated). Insertion of a plasmid containing the SCC\n            <jats:italic>mec att<\/jats:italic>\n            site (\n            <jats:italic>attS<\/jats:italic>\n            ) into the chromosome required both CcrA and CcrB, but CcrA overexpression lowered integration frequency. Thus, while CcrB binds DNA, interaction between CcrA and CcrB, in a precise ratio, is required for\n            <jats:italic>attB<\/jats:italic>\n            site-specific excision and SCC\n            <jats:italic>mec<\/jats:italic>\n            chromosomal insertion.\n          <\/jats:p>","DOI":"10.1128\/jb.01520-09","type":"journal-article","created":{"date-parts":[[2010,4,10]],"date-time":"2010-04-10T01:09:58Z","timestamp":1270861798000},"page":"3204-3212","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":61,"title":["Roles of CcrA and CcrB in Excision and Integration of Staphylococcal Cassette Chromosome\n            <i>mec<\/i>\n            , a\n            <i>Staphylococcus aureus<\/i>\n            Genomic Island"],"prefix":"10.1128","volume":"192","author":[{"given":"Lei","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Virginia Commonwealth University School of Medicine, McGuire Hall Room 103, 1112 East Clay Street, Richmond, Virginia 23298"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon L.","family":"Archer","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Virginia Commonwealth University School of Medicine, McGuire Hall Room 103, 1112 East Clay Street, Richmond, Virginia 23298"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"2001","DOI":"10.1073\/pnas.81.7.2001","volume":"81","year":"1984","unstructured":"Abdel-Meguid, S. 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